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Molecular and Cellular Biology, February 2001, p. 1329-1335, Vol. 21, No. 4
Department of
Biochemistry,1 Department of Biomedical
Regulation & Parasitology,2 and
Department of Physiology,4 School of
Medicine, Kobe University, Chuo-ku, Kobe 650-0017, Department
of Anatomy, Shimane Medical University, Izumo
693-8501,3 Department of Neuroanatomy
(D12), Graduate School of Medicine, Osaka University, Osaka
565-0871,5 and PREST (Precursory
Research for Embryonic Science and
Technology)6 and CREST (Core Research
for Evolutional Science and Technology)7 of
Japan Science and Technology Corporation (JST), Chiyoda-ku, Tokyo
102-0081, Japan
Received 14 August 2000/Returned for modification 14 September
2000/Accepted 13 November 2000
Although chromosomal segregation at meiosis I is the critical
process for genetic reassortment and inheritance, little is known about
molecules involved in this process in metazoa. Here we show by
utilizing double-stranded RNA (dsRNA)-mediated genetic interference
that novel protein kinases (Ce-CDS-1 and Ce-CDS-2) related to Cds1
(Chk2) play an essential role in meiotic recombination in
Caenorhabditis elegans. Injection of dsRNA into adult
animals resulted in the inhibition of meiotic crossing over and induced the loss of chiasmata at diakinesis in oocytes of F1
animals. However, electron microscopic analysis revealed that
synaptonemal complex formation in pachytene nuclei of the same
progeny of injected animals appeared to be normal. Thus, Ce-CDS-1 and
Ce-CDS-2 are the first example of Cds1-related kinases that are
required for meiotic recombination in multicellular organisms.
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.4.1329-1335.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Critical Role of Caenorhabditis elegans
Homologs of Cds1 (Chk2)-Related Kinases in Meiotic
Recombination
*
Corresponding author. Mailing address: Department of
Biomedical Regulation & Parasitology, Kobe University, School of
Medicine, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan. Phone:
81-78-382-5560. Fax: 81-78-382-5579. E-mail:
minami{at}kobe-u.ac.jp.
Molecular and Cellular Biology, February 2001, p. 1329-1335, Vol. 21, No. 4
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.4.1329-1335.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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