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Molecular and Cellular Biology, February 2001, p. 1370-1383, Vol. 21, No. 4
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.4.1370-1383.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

The PEA3 Subfamily of Ets Transcription Factors Synergizes with beta -Catenin-LEF-1 To Activate Matrilysin Transcription in Intestinal Tumors

Howard C. Crawford,1,* Barbara Fingleton,1 Mark D. Gustavson,1 Natasza Kurpios,2 Rebecca A. Wagenaar,1 John A. Hassell,2 and Lynn M. Matrisian1

Department of Cancer Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-2175,1 and Institute for Molecular Biology and Biotechnology, McMaster University Hamilton, Ontario, Canada L864K12

Received 5 October 2000/Returned for modification 31 October 2000/Accepted 9 November 2000

The matrix metalloproteinase matrilysin (MMP-7) is expressed in the tumor cells of a majority of mouse intestinal and human colonic adenomas. We showed previously that matrilysin is a target gene of beta -catenin-Tcf, the transcription factor complex whose activity is thought to play a crucial role in the initiation of intestinal tumorigenesis. Here we report that overexpression of a stable mutant form of beta -catenin alone was not sufficient to effect expression of luciferase from a matrilysin promoter-luciferase reporter plasmid. However, cotransfection of the reporter with an expression vector encoding the PEA3 Ets transcription factor, or its close relatives ER81 and ERM, increased luciferase expression and rendered the promoter responsive to beta -catenin-LEF-1 as well as to the AP-1 protein c-Jun. Other Ets proteins could not substitute for the PEA3 subfamily. Luciferase activity was induced up to 250-fold when PEA3, c-Jun, beta -catenin, and LEF-1 were coexpressed. This combination of transcription factors was also sufficient to induce expression of the endogenous matrilysin gene. Furthermore, all matrilysin-expressing benign intestinal tumors of the Min mouse expressed a member of the PEA3 subfamily, as did all human colon tumor cell lines examined. These data suggest that the expression of members of the PEA3 subfamily, in conjunction with the accumulation of beta -catenin in these tumors, leads to coordinate upregulation of matrilysin gene transcription, contributing to gastrointestinal tumorigenesis.

* Corresponding author. Mailing address: 1161 21st Ave. South, MCN T2219, Nashville, TN 37232-2175. Phone: (615) 343-3422. Fax: (615) 343-4539. E-mail: howard.crawford{at}mcmail.vanderbilt.edu.

Molecular and Cellular Biology, February 2001, p. 1370-1383, Vol. 21, No. 4
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.4.1370-1383.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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