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Molecular and Cellular Biology, April 2001, p. 2463-2466, Vol. 21, No. 7
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.7.2463-2466.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Functional Analysis of Mouse C-Terminal Kinesin Motor KifC2

Zhaohuai Yang, Elizabeth A. Roberts, and Lawrence S. B. Goldstein*

Department of Cellular and Molecular Medicine, Howard Hughes Medical Institute, University of California San Diego, La Jolla, California 92093

Received 20 December 2000/Accepted 3 January 2001

Proteins of the kinesin superfamily define a class of microtubule-dependent motors that play crucial roles in cell division and intracellular transport. In the mouse, several kinesin motors have been characterized and are suggested to play roles in axonal and/or dendritic transport. One such kinesin is KifC2. Sequence and secondary structure analysis revealed that KifC2 is a member of the C-terminal motor family. Northern and Western blot analyses indicated that KifC2 is specifically expressed in both the central and peripheral nervous systems. The cellular locations of the KifC2 proteins were found to be mainly in neural cell bodies and dendrites but also in axons. To understand the in vivo function of the KifC2 gene, we used homologous recombination in embryonic stem cells to construct knockout mouse strains for the KifC2 gene. Homozygous KifC2 mutants were viable and reproduced normally, and their development was apparently normal. These results suggest that KifC2 is dispensable for normal neural development and behavior in the mouse.

* Corresponding author. Mailing address: HHMI/CMM Room 334, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0683. Phone: (858) 534-9702. Fax: (858) 534-9701. E-mail: lgoldstein{at}ucsd.edu.

Molecular and Cellular Biology, April 2001, p. 2463-2466, Vol. 21, No. 7
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.7.2463-2466.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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