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Molecular and Cellular Biology, May 2001, p. 3083-3095, Vol. 21, No. 9
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.9.3083-3095.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Role of NF-Y in In Vivo Regulation of the gamma -Globin Gene

Zhijun Duan, George Stamatoyannopoulos, and Qiliang Li*

Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, Washington 98195

Received 13 September 2000/Returned for modification 25 October 2000/Accepted 9 February 2001

The duplicated CCAAT box is required for gamma  gene expression. We report here that the transcriptional factor NF-Y is recruited to the duplicated CCAAT box in vivo. A mutation of the duplicated CCAAT box that severely disrupts the NF-Y binding also reduces the accessibility level of the gamma  gene promoter, affects the assembly of basal transcriptional machinery, and increases the recruitment of GATA-1 to the locus control region (LCR) and the proximal promoter and the recruitment of transcription cofactor CBP/p300 to the LCR. These findings suggest that recruitment of NF-Y to the duplicated CCAAT box plays a role in the chromatin opening of the gamma  gene promoter as well as in the communication between the gamma  gene promoter and the LCR.

* Corresponding author. Mailing address: Medical Genetics, Box 357720, University of Washington, Seattle, WA 98195. Phone: (206)616-4526. Fax: (206)543-3050. E-mail: LI111640{at}u.washington.edu.

Molecular and Cellular Biology, May 2001, p. 3083-3095, Vol. 21, No. 9
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.9.3083-3095.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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