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Molecular and Cellular Biology, January 2002, p. 321-331, Vol. 22, No. 1
0270-7306/01/$04.00+0     DOI: 10.1128/MCB.22.1.321-331.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Interaction between P-TEFb and the C-Terminal Domain of RNA Polymerase II Activates Transcriptional Elongation from Sites Upstream or Downstream of Target Genes

Ran Taube, Xin Lin, Dan Irwin, Koh Fujinaga, and B. Matija Peterlin*

Howard Hughes Medical Institute, Departments of Medicine, Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143-0703

Received 18 June 2001/ Returned for modification 23 August 2001/ Accepted 1 October 2001

Transcriptional elongation by RNA polymerase II (RNAPII) is regulated by the positive transcription elongation factor b (P-TEFb). P-TEFb is composed of Cdk9 and C-type cyclin T1 (CycT1), CycT2a, CycT2b, or CycK. The role of the C-terminal region of CycT1 and CycT2 remains unknown. In this report, we demonstrate that these sequences are essential for the activation of transcription by P-TEFb via DNA, i.e., when CycT1 is tethered upstream or downstream of promoters and coding sequences. A histidine-rich stretch, which is conserved between CycT1 and CycT2 in this region, bound the C-terminal domain of RNAPII. This binding was required for the subsequent expression of full-length transcripts from target genes. Thus, P-TEFb could mediate effects of enhancers on the elongation of transcription.

* Corresponding author. Mailing address: Box 0703, N215 Mt. Zion Cancer Center, 2340 Sutter St., San Francisco, CA 94143-0703. Phone: (415) 502-1905. Fax: (415) 502-1901. E-mail: matija{at}itsa.ucsf.edu.

Molecular and Cellular Biology, January 2002, p. 321-331, Vol. 22, No. 1
0022-538X/01/$04.00+0     DOI: 10.1128/MCB.22.1.321-331.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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