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Molecular and Cellular Biology, June 2002, p. 3832-3841, Vol. 22, No. 11
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.11.3832-3841.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The Acetyltransferase Activity of CBP Is Required for wingless Activation and H4 Acetylation in Drosophila melanogaster

William H. Ludlam,1 Matthew H. Taylor,2 Kirk G. Tanner,3 John M. Denu,3 Richard H. Goodman,4 and Sarah M. Smolik5*

Department of Medicine,1 Department of Biochemistry and Molecular Biology,3 Vollum Institute,4 Department of Cell and Developmental Biology, Oregon Health & Science University, Portland, Oregon 97201,5 Department of Biology, Brigham Young University, Provo, Utah 846042

Received 11 December 2001/ Returned for modification 17 January 2002/ Accepted 31 January 2002

CBP is a critical coactivator of transcription, but little is understood about the importance of its intrinsic acetyltransferase (AT) activity in gene activation in vivo. We show that the intrinsic AT function of CBP in Drosophila melanogaster (dCBP) is necessary to maintain a dCBP overexpression phenotype in the eye, for the in vivo activation of a specific target gene, wingless, and for the global acetylation of histone H4. These findings indicate that a point mutation which alters the intrinsic AT activity of CBP (only one of many CBP functions) has profound effects on CBP-induced gene activation in a physiologically intact transcription system. Furthermore, the effects of CBP AT activity are not limited to a few specific promoters, but rather CBT AT activity may play a role in regulating global histone acetylation throughout the developing organism.

* Corresponding author. Mailing address: Oregon Health & Science University, 3181 S.W. Sam Jackson Park Rd., L-474, Portland, OR 97201. Phone: (503) 494-7192. Fax: (503) 494-4353. E-mail: smoliks{at}ohsu.edu.

Molecular and Cellular Biology, June 2002, p. 3832-3841, Vol. 22, No. 11
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.11.3832-3841.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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