This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wong, M. C. V. L.
Right arrow Articles by Murray, J. A. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wong, M. C. V. L.
Right arrow Articles by Murray, J. A. H.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, June 2002, p. 4218-4229, Vol. 22, No. 12
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.12.4218-4229.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

RSC2, Encoding a Component of the RSC Nucleosome Remodeling Complex, Is Essential for 2µm Plasmid Maintenance in Saccharomyces cerevisiae

Michael C. V. L. Wong,,{dagger} Suzanna R. S. Scott-Drew, Matthew J. Hayes,,{ddagger} Philip J. Howard, and James A. H. Murray*

Institute of Biotechnology, University of Cambridge, Cambridge CB2 1QT, United Kingdom

Received 8 October 2001/ Returned for modification 30 November 2001/ Accepted 10 March 2002

The stable maintenance of the 2µm circle plasmid depends on its ability to overcome intrinsic maternal inheritance bias, which in yeast normally results in the failure to transmit DNA molecules efficiently to daughter cells. In addition to the plasmid proteins Rep1 and Rep2 acting on the plasmid DNA locus STB, it is likely that other chromosomally encoded yeast proteins are required. We have isolated mutants of yeast unable to maintain 2µm and found that RSC2 is essential for 2µm to overcome maternal inheritance bias. Rsc2 is part of a multisubunit RSC chromatin remodeling complex, and we show that in the absence of Rsc2 the chromatin structure of the STB region is significantly altered and the Rep1 protein loses its normal localization to subnuclear foci. Rsc1, a closely related homolog of Rsc2 present in an alternative form of the RSC complex, is not required for 2µm maintenance and does not replace the requirement for Rsc2 when overexpressed. This represents the first specific role for Rsc2 that has been related to a change in chromatin structure, as well as the first direct evidence linking chromatin structure to 2µm segregation.

* Corresponding author. Mailing address: Institute of Biotechnology, University of Cambridge, Tennis Court Rd., Cambridge CB2 1QT, United Kingdom. Phone: 01223-334166. Fax: 01223-334167. E-mail: j.murray{at}biotech.cam.ac.uk.

{dagger} Present address: Department of Biotechnology, Faculty of Food Science and Biotechnology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

{ddagger} Present address: Department of Cell Biology, Institute of Ophthalmology, University College London, London EC1V 9EL, United Kingdom.

Molecular and Cellular Biology, June 2002, p. 4218-4229, Vol. 22, No. 12
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.12.4218-4229.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ghosh, S. K., Hajra, S., Jayaram, M. (2007). Faithful segregation of the multicopy yeast plasmid through cohesin-mediated recognition of sisters. Proc. Natl. Acad. Sci. USA 104: 13034-13039 [Abstract] [Full Text]  
  • Hajra, S., Ghosh, S. K., Jayaram, M. (2006). The centromere-specific histone variant Cse4p (CENP-A) is essential for functional chromatin architecture at the yeast 2-{micro}m circle partitioning locus and promotes equal plasmid segregation. JCB 174: 779-790 [Abstract] [Full Text]  
  • Malik, H. S. (2006). A hitchhiker's guide to survival finally makes CENs. JCB 174: 747-749 [Abstract] [Full Text]  
  • Shim, E. Y., Ma, J.-L., Oum, J.-H., Yanez, Y., Lee, S. E. (2005). The Yeast Chromatin Remodeler RSC Complex Facilitates End Joining Repair of DNA Double-Strand Breaks. Mol. Cell. Biol. 25: 3934-3944 [Abstract] [Full Text]  
  • Mehta, S., Yang, X.-M., Jayaram, M., Velmurugan, S. (2005). A Novel Role for the Mitotic Spindle during DNA Segregation in Yeast: Promoting 2{micro}m Plasmid-Cohesin Association. Mol. Cell. Biol. 25: 4283-4298 [Abstract] [Full Text]  
  • Chen, X. L., Reindle, A., Johnson, E. S. (2005). Misregulation of 2{micro}m Circle Copy Number in a SUMO Pathway Mutant. Mol. Cell. Biol. 25: 4311-4320 [Abstract] [Full Text]  
  • Yang, X.-M., Mehta, S., Uzri, D., Jayaram, M., Velmurugan, S. (2004). Mutations in a Partitioning Protein and Altered Chromatin Structure at the Partitioning Locus Prevent Cohesin Recruitment by the Saccharomyces cerevisiae Plasmid and Cause Plasmid Missegregation. Mol. Cell. Biol. 24: 5290-5303 [Abstract] [Full Text]  
  • Hsu, J.-m., Huang, J., Meluh, P. B., Laurent, B. C. (2003). The Yeast RSC Chromatin-Remodeling Complex Is Required for Kinetochore Function in Chromosome Segregation. Mol. Cell. Biol. 23: 3202-3215 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»