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 Previous Article

Molecular and Cellular Biology, June 2002, p. 4433-4438, Vol. 22, No. 12
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.12.4433-4438.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

A Mammal-Specific Exon of WT1 Is Not Required for Development or Fertility

Thomas A. Natoli,1,2 Alice McDonald,1,{dagger} Julia A. Alberta,3 Mary E. Taglienti,1 David E. Housman,3 and Jordan A. Kreidberg1,2*

Department of Medicine, The Children's Hospital,1 Department of Pediatrics, Harvard Medical School, Boston,2 Department of Biology, Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts3

Received 8 November 2001/ Returned for modification 27 December 2001/ Accepted 19 February 2002

The WT1 tumor suppressor gene is a zinc finger-containing transcription factor which is required for development of the kidney and gonads. A mammal-specific alternative splicing event within this gene results in the presence or absence of a 17-amino-acid sequence within the WT1 protein. To determine the function of this sequence in vivo, gene targeting was utilized to specifically eliminate the exon encoding this sequence in mice. Mice lacking WT1 exon 5 develop normally. Adult mice lacking this exon are viable and fertile, and females are capable of lactation.

* Corresponding author. Mailing address: Department of Medicine, The Children's Hospital, 300 Longwood Ave., Boston, MA 02115. Phone: (617) 247-5194. Fax: (617) 232-4315. E-mail: Kreidberg{at}tch.harvard.edu.

{dagger} Present address: Millennium Pharmaceuticals, Cambridge, MA.

Molecular and Cellular Biology, June 2002, p. 4433-4438, Vol. 22, No. 12
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.12.4433-4438.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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