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Molecular and Cellular Biology, July 2002, p. 4792-4802, Vol. 22, No. 13
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.13.4792-4802.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

A Physical Interaction between Gar1p and Rnt1p Is Required for the Nuclear Import of H/ACA Small Nucleolar RNA-Associated Proteins

Annie Tremblay,1 Bruno Lamontagne,1 Mathieu Catala,1 Yeung Yam,1 Stephanie Larose,1 Liam Good,2 and Sherif Abou Elela1*

Groupe ARN, Département de Microbiologie et d'Infectiologie, Faculté de Médecine, Université de Sherbrooke, Sherbrooke, Québec, Canada J1H 5N4,1 Center for Genomics and Bioinformatics, Karolinska Institutet, 171 77 Stockholm, Sweden2

Received 6 February 2002/ Returned for modification 8 March 2002/ Accepted 21 March 2002

During rRNA biogenesis, multiple RNA and protein substrates are modified and assembled through the coordinated activity of many factors. In Saccharomyces cerevisiae, the double-stranded RNA nuclease Rnt1p and the H/ACA snoRNA pseudouridylase complex participate in the transformation of the nascent pre-rRNA transcript into 35S pre-rRNA. Here we demonstrate the binding of a component of the H/ACA complex (Gar1p) to Rnt1p in vivo and in vitro in the absence of other factors. In vitro, Rnt1p binding to Gar1p is mutually exclusive of its RNA binding and cleavage activities. Mutations in Rnt1p that disrupt Gar1p binding do not inhibit RNA cleavage in vitro but slow RNA processing, prevent nucleolar localization of H/ACA snoRNA-associated proteins, and reduce pre-rRNA pseudouridylation in vivo. These results demonstrate colocalization of various components of the rRNA maturation complex and suggest a mechanism that links rRNA pseudouridylation and cleavage factors.

* Corresponding author. Mailing address: Groupe ARN, Département de Microbiologie et d'Infectiologie, Faculté de Médecine, Université de Sherbrooke, Sherbrooke, Québec, Canada J1H 5N4. Phone: (819) 564-5275. Fax: (819) 564-5392. E-mail: sabou{at}courrier.usherb.ca.

Molecular and Cellular Biology, July 2002, p. 4792-4802, Vol. 22, No. 13
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.13.4792-4802.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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