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Molecular and Cellular Biology, September 2002, p. 6046-6055, Vol. 22, No. 17
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.17.6046-6055.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Biogenesis of Yeast Telomerase Depends on the Importin Mtr10

Francisco Ferrezuelo,1 Barbara Steiner,2 Martí Aldea,3 and Bruce Futcher1*

Department of Molecular Genetics and Microbiology, State University of New York, Stony Brook, New York 11794-5222,1 Department of Pharmacology, University of Frankfurt, Biocenter Niederursel, Frankfurt, Germany,2 Departament de Ciències Mèdiques Bàsiques, Universitat de Lleida, 25198 Lleida, Spain3

Received 19 February 2002/ Returned for modification 25 March 2002/ Accepted 4 June 2002

Telomerase is a ribonucleoprotein particle (RNP) involved in chromosome end replication, but its biogenesis is poorly understood. The RNA component of yeast telomerase (Tlc1) is synthesized as a polyadenylated precursor and then processed to a mature poly(A)- form. We report here that the karyopherin Mtr10p is required for the normal accumulation of mature Tlc1 and its proper localization to the nucleus. Neither TLC1 transcription nor the stability of poly(A)- Tlc1 is significantly affected in mtr10{Delta} cells. Tlc1 was mostly nuclear in a wild-type background, and this localization was not affected by mutations in other telomerase components. Strikingly, in the absence of Mtr10p, Tlc1 was found dispersed throughout the entire cell. Our results are compatible with two alternative models. First, Mtr10p may import a cytoplasmic complex containing Tlc1 and perhaps other components of telomerase, and shuttling of Tlc1 from the nucleus to the cytoplasm and back may be necessary for the biogenesis of telomerase (the "shuttling" model). Second, Mtr10p may be necessary for the nuclear import of some enzyme needed for the nuclear processing and maturation of Tlc1, and in the absence of this maturation, poly(A)+ Tlc1 is aberrantly exported to the cytoplasm (the "processing enzyme" model).

* Corresponding author. Mailing address: Dept. Mol. Genetics & Microbiology, State University of New York, Stony Brook, NY 11794-5222. Phone: (631) 632-4715. Fax: (631) 632-9797. E-mail: bfutcher{at}ms.cc.sunysb.edu.

Molecular and Cellular Biology, September 2002, p. 6046-6055, Vol. 22, No. 17
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.17.6046-6055.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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