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Molecular and Cellular Biology, September 2002, p. 6417-6429, Vol. 22, No. 18
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.18.6417-6429.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Regulation of the Premiddle and Middle Phases of Expression of the NDT80 Gene during Sporulation of Saccharomyces cerevisiae

Julia Pak1 and Jacqueline Segall1,2*

Department of Molecular and Medical Genetics,1 Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada M5S 1A82

Received 5 April 2002/ Returned for modification 7 June 2002/ Accepted 21 June 2002

The NDT80 gene of Saccharomyces cerevisiae, which encodes a global activator of transcription of middle sporulation-specific genes, is first expressed after the activation of early meiotic genes but prior to activation of middle sporulation-specific genes. Both upstream repression sequence 1 (URS1) and mid-sporulation element (MSE) sites are present in the promoter region of the NDT80 gene; these elements have been shown previously to contribute to the regulation of expression of early and middle sporulation-specific genes, respectively, by mediating repression in growing cells and activation at specific times during sporulation. In this study, we have shown that the overlapping windows of URS1- and MSE-mediated repression and activation are responsible for the distinctive premiddle expression pattern of the NDT80 gene. Our data suggest that a Sum1-associated repression complex bound at the NDT80 MSE sites prevents Ime1 tethered at the NDT80 URS1 sites from activating transcription of the NDT80 gene at the time that Ime1-dependent activation of early URS1-regulated meiotic genes is occurring. We propose that a decrease in the efficiency of Sum1-mediated repression as cells progress through the early events of the sporulation program allows the previously inactive Ime1 tethered at the URS1NDT80 sites to promote a low level of expression of the NDT80 gene. This initial phase of URS1-dependent NDT80 expression is followed by Ndt80-dependent upregulation of its own expression, which requires the MSENDT80 sites and occurs concomitantly with Ndt80-dependent activation of a set of middle MSE-regulated sporulation-specific genes. Mutation of IME2 prevents expression of NDT80 in sporulating cells. We show in this study that NDT80 is expressed and that middle genes are activated in cells of an {Delta}ime2/{Delta}ime2 {Delta}sum1/{Delta}sum1 strain in sporulation medium. This suggests that Ime2 activates expression of NDT80 by eliminating Sum1-mediated repression.

* Corresponding author. Mailing address: Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada M5S 1A8. Phone: (416) 978-4981. Fax: (416) 978-8548. E-mail: j.segall{at}utoronto.ca.

Molecular and Cellular Biology, September 2002, p. 6417-6429, Vol. 22, No. 18
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.18.6417-6429.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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