This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Riedel, D.
Right arrow Articles by Jahn, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Riedel, D.
Right arrow Articles by Jahn, R.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 2002, p. 6487-6497, Vol. 22, No. 18
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.18.6487-6497.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Rab3D Is Not Required for Exocrine Exocytosis but for Maintenance of Normally Sized Secretory Granules

Dietmar Riedel,1 Wolfram Antonin,1 Rafael Fernandez-Chacon,2 Guillermo Alvarez de Toledo,2 Tobias Jo,1 Martin Geppert,3,{dagger} Jack A. Valentijn,4 Karin Valentijn,5 James D. Jamieson,5 Thomas C. Südhof,6 and Reinhard Jahn1*

Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen,1 Department of Molecular Neurobiology, Max Planck Institute for Experimental Medicine, 37073 Göttingen, Germany,3 Department of Medical Physiology and Biophysics, School of Medicine, University of Seville, 41009 Seville, Spain,2 Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510,5 Department of Biochemistry, Cell Biology and Histology, Faculty of Veterinary Medicine, University of Utrecht, 3584 CL Utrecht, The Netherlands,4 Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, Southwestern Medical Center, University of Texas, Dallas, Texas 753906

Received 19 February 2002/ Returned for modification 24 April 2002/ Accepted 12 June 2002

Rab3D, a member of the Rab3 subfamily of the Rab/ypt GTPases, is expressed on zymogen granules in the pancreas as well as on secretory vesicles in mast cells and in the parotid gland. To shed light on the function of Rab3D, we have generated Rab3D-deficient mice. These mice are viable and have no obvious phenotypic changes. Secretion of mast cells is normal as revealed by capacitance patch clamping. Furthermore, enzyme content and overall morphology are unchanged in pancreatic and parotid acinar cells of knockout mice. Both the exocrine pancreas and the parotid gland show normal release kinetics in response to secretagogue stimulation, suggesting that Rab3D is not involved in exocytosis. However, the size of secretory granules in both the exocrine pancreas and the parotid gland is significantly increased, with the volume being doubled. We conclude that Rab3D exerts its function during granule maturation, possibly by preventing homotypic fusion of secretory granules.

* Corresponding author. Mailing address: Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany. Phone: 49-551 201 1634. Fax: 49-551 201 1639. E-mail: rjahn{at}gwdg.de.

{dagger} Present address: Pharmacia Corporation, Kalamazoo, MI 49007.

Molecular and Cellular Biology, September 2002, p. 6487-6497, Vol. 22, No. 18
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.18.6487-6497.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Melicoff, E., Sansores-Garcia, L., Gomez, A., Moreira, D. C., Datta, P., Thakur, P., Petrova, Y., Siddiqi, T., Murthy, J. N., Dickey, B. F., Heidelberger, R., Adachi, R. (2009). Synaptotagmin-2 Controls Regulated Exocytosis but Not Other Secretory Responses of Mast Cells. J. Biol. Chem. 284: 19445-19451 [Abstract] [Full Text]  
  • Williams, J. A., Chen, X., Sabbatini, M. E. (2009). Small G proteins as key regulators of pancreatic digestive enzyme secretion. Am. J. Physiol. Endocrinol. Metab. 296: E405-E414 [Abstract] [Full Text]  
  • Marchelletta, R. R., Jacobs, D. T., Schechter, J. E., Cheney, R. E., Hamm-Alvarez, S. F. (2008). The class V myosin motor, myosin 5c, localizes to mature secretory vesicles and facilitates exocytosis in lacrimal acini. Am. J. Physiol. Cell Physiol. 295: C13-C28 [Abstract] [Full Text]  
  • Evans, E., Zhang, W., Jerdeva, G., Chen, C.-Y., Chen, X., Hamm-Alvarez, S. F., Okamoto, C. T. (2008). Direct interaction between Rab3D and the polymeric immunoglobulin receptor and trafficking through regulated secretory vesicles in lacrimal gland acinar cells. Am. J. Physiol. Cell Physiol. 294: C662-C674 [Abstract] [Full Text]  
  • Valentijn, J. A., van Weeren, L., Ultee, A., Koster, A. J. (2007). Novel localization of Rab3D in rat intestinal goblet cells and Brunner's gland acinar cells suggests a role in early Golgi trafficking. Am. J. Physiol. Gastrointest. Liver Physiol. 293: G165-G177 [Abstract] [Full Text]  
  • Rupnik, M., Kreft, M., Nothias, F., Grilc, S., Bobanovic, L. K., Johannes, L., Kiauta, T., Vernier, P., Darchen, F., Zorec, R. (2007). Distinct role of Rab3A and Rab3B in secretory activity of rat melanotrophs. Am. J. Physiol. Cell Physiol. 292: C98-C105 [Abstract] [Full Text]  
  • Rondaij, M. G., Bierings, R., Kragt, A., van Mourik, J. A., Voorberg, J. (2006). Dynamics and Plasticity of Weibel-Palade Bodies in Endothelial Cells. Arterioscler. Thromb. Vasc. Bio. 26: 1002-1007 [Abstract] [Full Text]  
  • Chen, X., Walker, A. K., Strahler, J. R., Simon, E. S., Tomanicek-Volk, S. L., Nelson, B. B., Hurley, M. C., Ernst, S. A., Williams, J. A., Andrews, P. C. (2006). Organellar Proteomics: Analysis of Pancreatic Zymogen Granule Membranes. Mol. Cell. Proteomics 5: 306-312 [Abstract] [Full Text]  
  • Pavlos, N. J., Xu, J., Riedel, D., Yeoh, J. S. G., Teitelbaum, S. L., Papadimitriou, J. M., Jahn, R., Ross, F. P., Zheng, M. H. (2005). Rab3D Regulates a Novel Vesicular Trafficking Pathway That Is Required for Osteoclastic Bone Resorption. Mol. Cell. Biol. 25: 5253-5269 [Abstract] [Full Text]  
  • Schluter, O. M., Schmitz, F., Jahn, R., Rosenmund, C., Sudhof, T. C. (2004). A Complete Genetic Analysis of Neuronal Rab3 Function. J. Neurosci. 24: 6629-6637 [Abstract] [Full Text]  
  • Matsumoto, M., Miki, T., Shibasaki, T., Kawaguchi, M., Shinozaki, H., Nio, J., Saraya, A., Koseki, H., Miyazaki, M., Iwanaga, T., Seino, S. (2004). Noc2 is essential in normal regulation of exocytosis in endocrine and exocrine cells. Proc. Natl. Acad. Sci. USA 101: 8313-8318 [Abstract] [Full Text]  
  • Imai, A., Yoshie, S., Nashida, T., Shimomura, H., Fukuda, M. (2004). The small GTPase Rab27B regulates amylase release from rat parotid acinar cells. J. Cell Sci. 117: 1945-1953 [Abstract] [Full Text]  
  • van Weeren, L., de Graaff, A. M., Jamieson, J. D., Batenburg, J. J., Valentijn, J. A. (2004). Rab3D and Actin Reveal Distinct Lamellar Body Subpopulations in Alveolar Epithelial Type II Cells. Am. J. Respir. Cell Mol. Bio. 30: 288-295 [Abstract] [Full Text]  
  • Tolmachova, T., Anders, R., Stinchcombe, J., Bossi, G., Griffiths, G. M., Huxley, C., Seabra, M. C. (2004). A General Role for Rab27a in Secretory Cells. Mol. Biol. Cell 15: 332-344 [Abstract] [Full Text]  
  • Chen, X., Ernst, S. A., Williams, J. A. (2003). Dominant Negative Rab3D Mutants Reduce GTP-bound Endogenous Rab3D in Pancreatic Acini. J. Biol. Chem. 278: 50053-50060 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»