This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Itoh, R. E.
Right arrow Articles by Matsuda, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Itoh, R. E.
Right arrow Articles by Matsuda, M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 2002, p. 6582-6591, Vol. 22, No. 18
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.18.6582-6591.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Activation of Rac and Cdc42 Video Imaged by Fluorescent Resonance Energy Transfer-Based Single-Molecule Probes in the Membrane of Living Cells

Reina E. Itoh,1 Kazuo Kurokawa,1 Yusuke Ohba,1,2 Hisayoshi Yoshizaki,1,2 Naoki Mochizuki,3 and Michiyuki Matsuda1*

Department of Tumor Virology, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871 ,1 CREST, Japan Science and Technology Cooperation,2 Department of Structural Analysis, National Cardiovascular Research Institute, Osaka 565-8565, Japan3

Received 17 January 2002/ Returned for modification 18 March 2002/ Accepted 20 June 2002

Rho family G proteins, including Rac and Cdc42, regulate a variety of cellular functions such as morphology, motility, and gene expression. We developed fluorescent resonance energy transfer-based probes which monitored the local balance between the activities of guanine nucleotide exchange factors and GTPase-activating proteins for Rac1 and Cdc42 at the membrane. These probes, named Raichu-Rac and Raichu-Cdc42, consisted of a Cdc42- and Rac-binding domain of Pak, Rac1 or Cdc42, a pair of green fluorescent protein mutants, and a CAAX box of Ki-Ras. With these probes, we video imaged the Rac and Cdc42 activities. In motile HT1080 cells, activities of both Rac and Cdc42 gradually increased toward the leading edge and decreased rapidly when cells changed direction. Under a higher magnification, we observed that Rac activity was highest immediately behind the leading edge, whereas Cdc42 activity was most prominent at the tip of the leading edge. Raichu-Rac and Raichu-Cdc42 were also applied to a rapid and simple assay for the analysis of putative guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs) in living cells. Among six putative GEFs and GAPs, we identified KIAA0362/DBS as a GEF for Rac and Cdc42, KIAA1256 as a GEF for Cdc42, KIAA0053 as a GAP for Rac and Cdc42, and KIAA1204 as a GAP for Cdc42. In conclusion, use of these single-molecule probes to determine Rac and Cdc42 activity will accelerate the analysis of the spatiotemporal regulation of Rac and Cdc42 in a living cell.

* Corresponding author. Mailing address: Department of Tumor Virology, Research Institute for Microbial Diseases, Osaka University, Yamadaoka, Suita-shi, Osaka 565-0871, Japan. Phone: 81-6-6879-8316. Fax: 81-6-6879-8314. E-mail: matsudam{at}biken.osaka-u.ac.jp.

Molecular and Cellular Biology, September 2002, p. 6582-6591, Vol. 22, No. 18
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.18.6582-6591.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Yu, J. A., Deakin, N. O., Turner, C. E. (2009). Paxillin-Kinase-Linker Tyrosine Phosphorylation Regulates Directional Cell Migration. Mol. Biol. Cell 20: 4706-4719 [Abstract] [Full Text]  
  • Meiri, D., Greeve, M. A., Brunet, A., Finan, D., Wells, C. D., LaRose, J., Rottapel, R. (2009). Modulation of Rho Guanine Exchange Factor Lfc Activity by Protein Kinase A-Mediated Phosphorylation. Mol. Cell. Biol. 29: 5963-5973 [Abstract] [Full Text]  
  • Sin, W.-C., Tse, M., Planque, N., Perbal, B., Lampe, P. D., Naus, C. C. (2009). Matricellular Protein CCN3 (NOV) Regulates Actin Cytoskeleton Reorganization. J. Biol. Chem. 284: 29935-29944 [Abstract] [Full Text]  
  • Goldyn, A. M., Rioja, B. A., Spatz, J. P., Ballestrem, C., Kemkemer, R. (2009). Force-induced cell polarisation is linked to RhoA-driven microtubule-independent focal-adhesion sliding. J. Cell Sci. 122: 3644-3651 [Abstract] [Full Text]  
  • Parrini, M. C., Camonis, J., Matsuda, M., de Gunzburg, J. (2009). Dissecting Activation of the PAK1 Kinase at Protrusions in Living Cells. J. Biol. Chem. 284: 24133-24143 [Abstract] [Full Text]  
  • Kawada, K., Upadhyay, G., Ferandon, S., Janarthanan, S., Hall, M., Vilardaga, J.-P., Yajnik, V. (2009). Cell Migration Is Regulated by Platelet-Derived Growth Factor Receptor Endocytosis. Mol. Cell. Biol. 29: 4508-4518 [Abstract] [Full Text]  
  • Elfenbein, A., Rhodes, J. M., Meller, J., Schwartz, M. A., Matsuda, M., Simons, M. (2009). Suppression of RhoG activity is mediated by a syndecan 4-synectin-RhoGDI1 complex and is reversed by PKC{alpha} in a Rac1 activation pathway. JCB 186: 75-83 [Abstract] [Full Text]  
  • Makrogianneli, K., Carlin, L. M., Keppler, M. D., Matthews, D. R., Ofo, E., Coolen, A., Ameer-Beg, S. M., Barber, P. R., Vojnovic, B., Ng, T. (2009). Integrating Receptor Signal Inputs That Influence Small Rho GTPase Activation Dynamics at the Immunological Synapse. Mol. Cell. Biol. 29: 2997-3006 [Abstract] [Full Text]  
  • Deakin, N. O., Bass, M. D., Warwood, S., Schoelermann, J., Mostafavi-Pour, Z., Knight, D., Ballestrem, C., Humphries, M. J. (2009). An integrin-{alpha}4-14-3-3{zeta}-paxillin ternary complex mediates localised Cdc42 activity and accelerates cell migration. J. Cell Sci. 122: 1654-1664 [Abstract] [Full Text]  
  • Na, S., Chowdhury, F., Tay, B., Ouyang, M., Gregor, M., Wang, Y., Wiche, G., Wang, N. (2009). Plectin contributes to mechanical properties of living cells. Am. J. Physiol. Cell Physiol. 296: C868-C877 [Abstract] [Full Text]  
  • Barber, P.R, Ameer-Beg, S.M, Gilbey, J, Carlin, L.M, Keppler, M, Ng, T.C, Vojnovic, B (2009). Multiphoton time-domain fluorescence lifetime imaging microscopy: practical application to protein-protein interactions using global analysis. J R Soc Interface 6: S93-S105 [Abstract] [Full Text]  
  • Stuart, H. C., Jia, Z., Messenberg, A., Joshi, B., Underhill, T. M., Moukhles, H., Nabi, I. R. (2008). Localized Rho GTPase Activation Regulates RNA Dynamics and Compartmentalization in Tumor Cell Protrusions. J. Biol. Chem. 283: 34785-34795 [Abstract] [Full Text]  
  • Bass, M. D., Morgan, M. R., Roach, K. A., Settleman, J., Goryachev, A. B., Humphries, M. J. (2008). p190RhoGAP is the convergence point of adhesion signals from {alpha}5{beta}1 integrin and syndecan-4. JCB 181: 1013-1026 [Abstract] [Full Text]  
  • Nishioka, T., Aoki, K., Hikake, K., Yoshizaki, H., Kiyokawa, E., Matsuda, M. (2008). Rapid Turnover Rate of Phosphoinositides at the Front of Migrating MDCK Cells. Mol. Biol. Cell 19: 4213-4223 [Abstract] [Full Text]  
  • Ouyang, M., Sun, J., Chien, S., Wang, Y. (2008). Determination of hierarchical relationship of Src and Rac at subcellular locations with FRET biosensors. Proc. Natl. Acad. Sci. USA 105: 14353-14358 [Abstract] [Full Text]  
  • Yamazaki, Y., Umeda, K., Wada, M., Nada, S., Okada, M., Tsukita, S., Tsukita, S. (2008). ZO-1- and ZO-2-Dependent Integration of Myosin-2 to Epithelial Zonula Adherens. Mol. Biol. Cell 19: 3801-3811 [Abstract] [Full Text]  
  • Na, S., Wang, N. (2008). Application of Fluorescence Resonance Energy Transfer and Magnetic Twisting Cytometry to Quantify Mechanochemical Signaling Activities in a Living Cell. Sci Signal 1: pl1-pl1 [Abstract] [Full Text]  
  • Itoh, R. E., Kiyokawa, E., Aoki, K., Nishioka, T., Akiyama, T., Matsuda, M. (2008). Phosphorylation and activation of the Rac1 and Cdc42 GEF Asef in A431 cells stimulated by EGF. J. Cell Sci. 121: 2635-2642 [Abstract] [Full Text]  
  • Pouthas, F., Girard, P., Lecaudey, V., Ly, T. B. N., Gilmour, D., Boulin, C., Pepperkok, R., Reynaud, E. G. (2008). In migrating cells, the Golgi complex and the position of the centrosome depend on geometrical constraints of the substratum. J. Cell Sci. 121: 2406-2414 [Abstract] [Full Text]  
  • Nakaya, M., Kitano, M., Matsuda, M., Nagata, S. (2008). Spatiotemporal activation of Rac1 for engulfment of apoptotic cells. Proc. Natl. Acad. Sci. USA 105: 9198-9203 [Abstract] [Full Text]  
  • Aoki, K., Kiyokawa, E., Nakamura, T., Matsuda, M. (2008). Visualization of growth signal transduction cascades in living cells with genetically encoded probes based on Forster resonance energy transfer. Phil Trans R Soc B 363: 2143-2151 [Abstract] [Full Text]  
  • Smith, T. K., Hager, H. A., Francis, R., Kilkenny, D. M., Lo, C. W., Bader, D. M. (2008). Bves directly interacts with GEFT, and controls cell shape and movement through regulation of Rac1/Cdc42 activity. Proc. Natl. Acad. Sci. USA 105: 8298-8303 [Abstract] [Full Text]  
  • Xia, N., Thodeti, C. K., Hunt, T. P., Xu, Q., Ho, M., Whitesides, G. M., Westervelt, R., Ingber, D. E. (2008). Directional control of cell motility through focal adhesion positioning and spatial control of Rac activation. FASEB J. 22: 1649-1659 [Abstract] [Full Text]  
  • Matthews, H. K., Marchant, L., Carmona-Fontaine, C., Kuriyama, S., Larrain, J., Holt, M. R., Parsons, M., Mayor, R. (2008). Directional migration of neural crest cells in vivo is regulated by Syndecan-4/Rac1 and non-canonical Wnt signaling/RhoA. Development 135: 1771-1780 [Abstract] [Full Text]  
  • Perez, T. D., Tamada, M., Sheetz, M. P., Nelson, W. J. (2008). Immediate-Early Signaling Induced by E-cadherin Engagement and Adhesion. J. Biol. Chem. 283: 5014-5022 [Abstract] [Full Text]  
  • Zhang, S., Schafer-Hales, K., Khuri, F. R., Zhou, W., Vertino, P. M., Marcus, A. I. (2008). The Tumor Suppressor LKB1 Regulates Lung Cancer Cell Polarity by Mediating cdc42 Recruitment and Activity. Cancer Res. 68: 740-748 [Abstract] [Full Text]  
  • Babbin, B. A., Jesaitis, A. J., Ivanov, A. I., Kelly, D., Laukoetter, M., Nava, P., Parkos, C. A., Nusrat, A. (2007). Formyl Peptide Receptor-1 Activation Enhances Intestinal Epithelial Cell Restitution through Phosphatidylinositol 3-Kinase-Dependent Activation of Rac1 and Cdc42. J. Immunol. 179: 8112-8121 [Abstract] [Full Text]  
  • Wong, H. L., Pinontoan, R., Hayashi, K., Tabata, R., Yaeno, T., Hasegawa, K., Kojima, C., Yoshioka, H., Iba, K., Kawasaki, T., Shimamoto, K. (2007). Regulation of Rice NADPH Oxidase by Binding of Rac GTPase to Its N-Terminal Extension. Plant Cell 19: 4022-4034 [Abstract] [Full Text]  
  • Prag, S., Parsons, M., Keppler, M. D., Ameer-Beg, S. M., Barber, P., Hunt, J., Beavil, A. J., Calvert, R., Arpin, M., Vojnovic, B., Ng, T. (2007). Activated Ezrin Promotes Cell Migration through Recruitment of the GEF Dbl to Lipid Rafts and Preferential Downstream Activation of Cdc42. Mol. Biol. Cell 18: 2935-2948 [Abstract] [Full Text]  
  • Yamada, S., Nelson, W. J. (2007). Localized zones of Rho and Rac activities drive initiation and expansion of epithelial cell cell adhesion. JCB 178: 517-527 [Abstract] [Full Text]  
  • Aoki, K., Nakamura, T., Inoue, T., Meyer, T., Matsuda, M. (2007). An essential role for the SHIP2-dependent negative feedback loop in neuritogenesis of nerve growth factor-stimulated PC12 cells. JCB 177: 817-827 [Abstract] [Full Text]  
  • Bass, M. D., Roach, K. A., Morgan, M. R., Mostafavi-Pour, Z., Schoen, T., Muramatsu, T., Mayer, U., Ballestrem, C., Spatz, J. P., Humphries, M. J. (2007). Syndecan-4-dependent Rac1 regulation determines directional migration in response to the extracellular matrix. JCB 177: 527-538 [Abstract] [Full Text]  
  • Bourzac, K. M., Botham, C. M., Guillemin, K. (2007). Helicobacter pylori CagA Induces AGS Cell Elongation through a Cell Retraction Defect That Is Independent of Cdc42, Rac1, and Arp2/3. Infect. Immun. 75: 1203-1213 [Abstract] [Full Text]  
  • Murata, T., Ohnishi, H., Okazawa, H., Murata, Y., Kusakari, S., Hayashi, Y., Miyashita, M., Itoh, H., Oldenborg, P.-A., Furuya, N., Matozaki, T. (2006). CD47 Promotes Neuronal Development through Src- and FRG/Vav2-Mediated Activation of Rac and Cdc42. J. Neurosci. 26: 12397-12407 [Abstract] [Full Text]  
  • Shin, E.-Y., Lee, C.-S., Cho, T. G., Kim, Y. G., Song, S., Juhnn, Y.-S., Park, S. C., Manser, E., Kim, E.-G. (2006). betaPak-interacting Exchange Factor-mediated Rac1 Activation Requires smgGDS Guanine Nucleotide Exchange Factor in Basic Fibroblast Growth Factor-induced Neurite Outgrowth. J. Biol. Chem. 281: 35954-35964 [Abstract] [Full Text]  
  • Wang, D., Tan, Y.-c., Kreitzer, G. E., Nakai, Y., Shan, D., Zheng, Y., Huang, X.-Y. (2006). G Proteins G12 and G13 Control the Dynamic Turnover of Growth Factor-induced Dorsal Ruffles. J. Biol. Chem. 281: 32660-32667 [Abstract] [Full Text]  
  • Yamana, N., Arakawa, Y., Nishino, T., Kurokawa, K., Tanji, M., Itoh, R. E., Monypenny, J., Ishizaki, T., Bito, H., Nozaki, K., Hashimoto, N., Matsuda, M., Narumiya, S. (2006). The Rho-mDia1 Pathway Regulates Cell Polarity and Focal Adhesion Turnover in Migrating Cells through Mobilizing Apc and c-Src.. Mol. Cell. Biol. 26: 6844-6858 [Abstract] [Full Text]  
  • Sakakibara, A., Horwitz, A. F. (2006). Mechanism of polarized protrusion formation on neuronal precursors migrating in the developing chicken cerebellum. J. Cell Sci. 119: 3583-3592 [Abstract] [Full Text]  
  • Watanabe, T., Tsuda, M., Makino, Y., Ichihara, S., Sawa, H., Minami, A., Mochizuki, N., Nagashima, K., Tanaka, S. (2006). Adaptor Molecule Crk Is Required for Sustained Phosphorylation of Grb2-Associated Binder 1 and Hepatocyte Growth Factor-Induced Cell Motility of Human Synovial Sarcoma Cell Lines. Mol Cancer Res 4: 499-510 [Abstract] [Full Text]  
  • Chenette, E. J., Mitin, N. Y., Der, C. J. (2006). Multiple Sequence Elements Facilitate Chp Rho GTPase Subcellular Location, Membrane Association, and Transforming Activity. Mol. Biol. Cell 17: 3108-3121 [Abstract] [Full Text]  
  • Nishimura, A., Okamoto, M., Sugawara, Y., Mizuno, N., Yamauchi, J., Itoh, H. (2006). Ric-8A potentiates Gq-mediated signal transduction by acting downstream of G protein-coupled receptor in intact cells.. GENES CELLS 11: 487-498 [Abstract] [Full Text]  
  • Nakai, Y., Zheng, Y., MacCollin, M., Ratner, N. (2006). Temporal control of Rac in Schwann cell-axon interaction is disrupted in NF2-mutant schwannoma cells.. J. Neurosci. 26: 3390-3395 [Abstract] [Full Text]  
  • ten Klooster, J. P., Jaffer, Z. M., Chernoff, J., Hordijk, P. L. (2006). Targeting and activation of Rac1 are mediated by the exchange factor {beta}-Pix. JCB 172: 759-769 [Abstract] [Full Text]  
  • Kuo, J.-C., Wang, W.-J., Yao, C.-C., Wu, P.-R., Chen, R.-H. (2006). The tumor suppressor DAPK inhibits cell motility by blocking the integrin-mediated polarity pathway.. JCB 172: 619-631 [Abstract] [Full Text]  
  • Reville, K., Crean, J. K., Vivers, S., Dransfield, I., Godson, C. (2006). Lipoxin A4 Redistributes Myosin IIA and Cdc42 in Macrophages: Implications for Phagocytosis of Apoptotic Leukocytes. J. Immunol. 176: 1878-1888 [Abstract] [Full Text]  
  • Sasuga, Y., Tani, T., Hayashi, M., Yamakawa, H., Ohara, O., Harada, Y. (2006). Development of a microscopic platform for real-time monitoring of biomolecular interactions. Genome Res 16: 132-139 [Abstract] [Full Text]  
  • Kurokawa, K., Matsuda, M. (2005). Localized RhoA Activation as a Requirement for the Induction of Membrane Ruffling. Mol. Biol. Cell 16: 4294-4303 [Abstract] [Full Text]  
  • Pankov, R., Endo, Y., Even-Ram, S., Araki, M., Clark, K., Cukierman, E., Matsumoto, K., Yamada, K. M. (2005). A Rac switch regulates random versus directionally persistent cell migration. JCB 170: 793-802 [Abstract] [Full Text]  
  • Aoki, K., Nakamura, T., Fujikawa, K., Matsuda, M. (2005). Local Phosphatidylinositol 3,4,5-Trisphosphate Accumulation Recruits Vav2 and Vav3 to Activate Rac1/Cdc42 and Initiate Neurite Outgrowth in Nerve Growth Factor-stimulated PC12 Cells. Mol. Biol. Cell 16: 2207-2217 [Abstract] [Full Text]  
  • Zhang, H., Webb, D. J., Asmussen, H., Niu, S., Horwitz, A. F. (2005). A GIT1/PIX/Rac/PAK Signaling Module Regulates Spine Morphogenesis and Synapse Formation through MLC. J. Neurosci. 25: 3379-3388 [Abstract] [Full Text]  
  • Pratt, S. J., Epple, H., Ward, M., Feng, Y., Braga, V. M., Longmore, G. D. (2005). The LIM protein Ajuba influences p130Cas localization and Rac1 activity during cell migration. JCB 168: 813-824 [Abstract] [Full Text]  
  • Fujita, H., Fukuhara, S., Sakurai, A., Yamagishi, A., Kamioka, Y., Nakaoka, Y., Masuda, M., Mochizuki, N. (2005). Local Activation of Rap1 Contributes to Directional Vascular Endothelial Cell Migration Accompanied by Extension of Microtubules on Which RAPL, a Rap1-associating Molecule, Localizes. J. Biol. Chem. 280: 5022-5031 [Abstract] [Full Text]  
  • Khyrul, W. A. K. M., LaLonde, D. P., Brown, M. C., Levinson, H., Turner, C. E. (2004). The Integrin-linked Kinase Regulates Cell Morphology and Motility in a Rho-associated Kinase-dependent Manner. J. Biol. Chem. 279: 54131-54139 [Abstract] [Full Text]  
  • Sai, J., Fan, G.-H., Wang, D., Richmond, A. (2004). The C-terminal domain LLKIL motif of CXCR2 is required for ligand-mediated polarization of early signals during chemotaxis. J. Cell Sci. 117: 5489-5496 [Abstract] [Full Text]  
  • Kodama, A., Lechler, T., Fuchs, E. (2004). Coordinating cytoskeletal tracks to polarize cellular movements. JCB 167: 203-207 [Abstract] [Full Text]  
  • Yoshizaki, H., Ohba, Y., Parrini, M.-C., Dulyaninova, N. G., Bresnick, A. R., Mochizuki, N., Matsuda, M. (2004). Cell Type-specific Regulation of RhoA Activity during Cytokinesis. J. Biol. Chem. 279: 44756-44762 [Abstract] [Full Text]  
  • Nalbant, P., Hodgson, L., Kraynov, V., Toutchkine, A., Hahn, K. M. (2004). Activation of Endogenous Cdc42 Visualized in Living Cells. Science 305: 1615-1619 [Abstract] [Full Text]  
  • Fukuhara, T., Shimizu, K., Kawakatsu, T., Fukuyama, T., Minami, Y., Honda, T., Hoshino, T., Yamada, T., Ogita, H., Okada, M., Takai, Y. (2004). Activation of Cdc42 by trans interactions of the cell adhesion molecules nectins through c-Src and Cdc42-GEF FRG. JCB 166: 393-405 [Abstract] [Full Text]  
  • Yano, H., Mazaki, Y., Kurokawa, K., Hanks, S. K., Matsuda, M., Sabe, H. (2004). Roles played by a subset of integrin signaling molecules in cadherin-based cell-cell adhesion. JCB 166: 283-295 [Abstract] [Full Text]  
  • Takaya, A., Ohba, Y., Kurokawa, K., Matsuda, M. (2004). RalA Activation at Nascent Lamellipodia of Epidermal Growth Factor-stimulated Cos7 Cells and Migrating Madin-Darby Canine Kidney Cells. Mol. Biol. Cell 15: 2549-2557 [Abstract] [Full Text]  
  • Ikeda, W., Kakunaga, S., Takekuni, K., Shingai, T., Satoh, K., Morimoto, K., Takeuchi, M., Imai, T., Takai, Y. (2004). Nectin-like Molecule-5/Tage4 Enhances Cell Migration in an Integrin-dependent, Nectin-3-independent Manner. J. Biol. Chem. 279: 18015-18025 [Abstract] [Full Text]  
  • Pertz, O., Hahn, K. M. (2004). Designing biosensors for Rho family proteins -- deciphering the dynamics of Rho family GTPase activation in living cells. J. Cell Sci. 117: 1313-1318 [Abstract] [Full Text]  
  • van Bruggen, R., Anthony, E., Fernandez-Borja, M., Roos, D. (2004). Continuous Translocation of Rac2 and the NADPH Oxidase Component p67phox during Phagocytosis. J. Biol. Chem. 279: 9097-9102 [Abstract] [Full Text]  
  • Korlach, J., Baird, D. W., Heikal, A. A., Gee, K. R., Hoffman, G. R., Webb, W. W. (2004). Spontaneous nucleotide exchange in low molecular weight GTPases by fluorescently labeled {gamma}-phosphate-linked GTP analogs. Proc. Natl. Acad. Sci. USA 101: 2800-2805 [Abstract] [Full Text]  
  • Kurokawa, K., Itoh, R. E., Yoshizaki, H., Nakamura, Y. O. T., Matsuda, M. (2004). Coactivation of Rac1 and Cdc42 at Lamellipodia and Membrane Ruffles Induced by Epidermal Growth Factor. Mol. Biol. Cell 15: 1003-1010 [Abstract] [Full Text]  
  • Hoshino, T., Shimizu, K., Honda, T., Kawakatsu, T., Fukuyama, T., Nakamura, T., Matsuda, M., Takai, Y. (2004). A Novel Role of Nectins in Inhibition of the E-Cadherin-induced Activation of Rac and Formation of Cell-Cell Adherens Junctions. Mol. Biol. Cell 15: 1077-1088 [Abstract] [Full Text]  
  • Matsuoka, H., Nada, S., Okada, M. (2004). Mechanism of Csk-mediated Down-regulation of Src Family Tyrosine Kinases in Epidermal Growth Factor Signaling. J. Biol. Chem. 279: 5975-5983 [Abstract] [Full Text]  
  • Grimsley, C. M., Kinchen, J. M., Tosello-Trampont, A.-C., Brugnera, E., Haney, L. B., Lu, M., Chen, Q., Klingele, D., Hengartner, M. O., Ravichandran, K. S. (2004). Dock180 and ELMO1 Proteins Cooperate to Promote Evolutionarily Conserved Rac-dependent Cell Migration. J. Biol. Chem. 279: 6087-6097 [Abstract] [Full Text]  
  • Aoki, K., Nakamura, T., Matsuda, M. (2004). Spatio-temporal Regulation of Rac1 and Cdc42 Activity during Nerve Growth Factor-induced Neurite Outgrowth in PC12 Cells. J. Biol. Chem. 279: 713-719 [Abstract] [Full Text]  
  • Ridley, A. J., Schwartz, M. A., Burridge, K., Firtel, R. A., Ginsberg, M. H., Borisy, G., Parsons, J. T., Horwitz, A. R. (2003). Cell Migration: Integrating Signals from Front to Back. Science 302: 1704-1709 [Abstract] [Full Text]  
  • Tzima, E., Kiosses, W. B., del Pozo, M. A., Schwartz, M. A. (2003). Localized Cdc42 Activation, Detected Using a Novel Assay, Mediates Microtubule Organizing Center Positioning in Endothelial Cells in Response to Fluid Shear Stress. J. Biol. Chem. 278: 31020-31023 [Abstract] [Full Text]  
  • Yoshizaki, H., Ohba, Y., Kurokawa, K., Itoh, R. E., Nakamura, T., Mochizuki, N., Nagashima, K., Matsuda, M. (2003). Activity of Rho-family GTPases during cell division as visualized with FRET-based probes. JCB 162: 223-232 [Abstract] [Full Text]  
  • Li, L., Guris, D. L., Okura, M., Imamoto, A. (2003). Translocation of CrkL to Focal Adhesions Mediates Integrin-Induced Migration Downstream of Src Family Kinases. Mol. Cell. Biol. 23: 2883-2892 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»