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Molecular and Cellular Biology, October 2002, p. 6669-6680, Vol. 22, No. 19
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.19.6669-6680.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Isolation and Characterization of New Proliferating Cell Nuclear Antigen (POL30) Mutator Mutants That Are Defective in DNA Mismatch Repair

Patrick J. Lau, Hernan Flores-Rozas, and Richard D. Kolodner*

Ludwig Institute for Cancer Research, Cancer Center, and Department of Medicine, University of California-San Diego School of Medicine, La Jolla, California 92093-0660

Received 28 February 2002/ Returned for modification 31 March 2002/ Accepted 28 June 2002

A number of studies have suggested a role for proliferating cell nuclear antigen (PCNA) in DNA mismatch repair (MMR). However, the majority of mutations in the POL30 gene encoding PCNA that cause MMR defects also cause replication and other repair defects that contribute to the increased mutation rate caused by these mutations. Here, 20 new pol30 mutants were identified and screened for MMR and other defects, resulting in the identification of two mutations, pol30-201 and pol30-204, that appear to cause MMR defects but little if any other defects. The pol30-204 mutation altered an amino acid (C81R) in the monomer-monomer interface region and resulted in a partial general MMR defect and a defect in MSH2-MSH6 binding in vitro. The pol30-201 mutation altered an amino acid (C22Y) located on the surface of the PCNA trimer that slides over the DNA but did not cause a defect in MSH2-MSH6 binding in vitro. The pol30-201 mutation caused an intermediate mutator phenotype. However, the pol30-201 mutation caused almost a complete defect in the repair of AC and GT mispairs and only a small defect in the repair of a "+T" insertion, an effect similar to that caused by an msh6{Delta} mutation, indicating that pol30-201 primarily effects MSH6-dependent MMR. The chromosomal double mutant msh3-FF>AA msh6-FF>AA eliminating the conserved FF residues of the PCNA interacting motif of these proteins caused a small (<10%) defect in MMR but showed synergistic interactions with mutations in POL30, indicating that the FF>AA substitution may not eliminate PCNA interactions in vivo. These results indicate that the interaction between PCNA and MMR proteins is more complex than was previously appreciated.

* Corresponding author. Mailing address: Ludwig Institute for Cancer Research, UCSD School of Medicine-CMME3080, 9500 Gilman Dr., La Jolla, CA 92093-0660. Phone: (858) 534-7804. Fax: (858) 534-7750. E-mail: rkolodner{at}ucsd.edu.

Molecular and Cellular Biology, October 2002, p. 6669-6680, Vol. 22, No. 19
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.19.6669-6680.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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