This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Åkerblad, P.
Right arrow Articles by Sigvardsson, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Åkerblad, P.
Right arrow Articles by Sigvardsson, M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, November 2002, p. 8015-8025, Vol. 22, No. 22
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.22.8015-8025.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Early B-Cell Factor (O/E-1) Is a Promoter of Adipogenesis and Involved in Control of Genes Important for Terminal Adipocyte Differentiation

Peter Åkerblad,1* Ulrika Lind,1 David Liberg,2 Krister Bamberg,1 and Mikael Sigvardsson2

Department of Molecular Biology, AstraZeneca R & D Mölndal, SE-431 83 Mölndal,1 Department for Stem Cell Biology, Lund University, SE-221 84 Lund, Sweden2

Received 6 February 2002/ Returned for modification 14 May 2002/ Accepted 19 August 2002

Olf-1/early B-cell factor (O/E-1) is a transcription factor important for B-lymphocyte and neuronal gene regulation. Here we report that all three known O/E genes (O/E-1, -2, and -3) are expressed in mouse adipose tissue and are upregulated during adipocyte differentiation. Forced expression of O/E-1 in either the preadipocyte cell line 3T3-L1 or mouse embryonic fibroblasts augmented adipogenesis, and constitutive expression of O/E-1 in uncommitted NIH 3T3 fibroblasts led to initiation of adipocyte differentiation. Furthermore, a dominant negative form of O/E-1 partially suppressed 3T3-L1 adipogenesis, indicating that expression from endogenous O/E target genes is required for 3T3-L1 terminal differentiation. Thus, our data point to the importance of O/E target genes for adipocyte differentiation and suggest a novel role for O/E-1 as an initiator and stimulator of adipogenesis.

* Corresponding author. Mailing address: Department of Molecular Biology, AstraZeneca R & D Mölndal, SE-431 83 Mölndal, Sweden. Phone: 46 317 064 493. Fax: 46 317 763 852. E-mail: peter.akerblad{at}astrazeneca.com.

Molecular and Cellular Biology, November 2002, p. 8015-8025, Vol. 22, No. 22
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.22.8015-8025.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Chen, W., Yechoor, V. K., Chang, B. H.-J., Li, M. V., March, K. L., Chan, L. (2009). The Human Lipodystrophy Gene Product Berardinelli-Seip Congenital Lipodystrophy 2/Seipin Plays a Key Role in Adipocyte Differentiation. Endocrinology 150: 4552-4561 [Abstract] [Full Text]  
  • Lorenzo, J., Horowitz, M., Choi, Y. (2008). Osteoimmunology: Interactions of the Bone and Immune System. Endocr. Rev. 29: 403-440 [Abstract] [Full Text]  
  • Xu, Z., Yu, S., Hsu, C.-H., Eguchi, J., Rosen, E. D. (2008). The orphan nuclear receptor chicken ovalbumin upstream promoter-transcription factor II is a critical regulator of adipogenesis. Proc. Natl. Acad. Sci. USA 105: 2421-2426 [Abstract] [Full Text]  
  • Kang, S., Bennett, C. N., Gerin, I., Rapp, L. A., Hankenson, K. D., MacDougald, O. A. (2007). Wnt Signaling Stimulates Osteoblastogenesis of Mesenchymal Precursors by Suppressing CCAAT/Enhancer-binding Protein {alpha} and Peroxisome Proliferator-activated Receptor {gamma}. J. Biol. Chem. 282: 14515-14524 [Abstract] [Full Text]  
  • Lagergren, A., Mansson, R., Zetterblad, J., Smith, E., Basta, B., Bryder, D., Akerblad, P., Sigvardsson, M. (2007). The Cxcl12, Periostin, and Ccl9 Genes Are Direct Targets for Early B-cell Factor in OP-9 Stroma Cells. J. Biol. Chem. 282: 14454-14462 [Abstract] [Full Text]  
  • Jimenez, M. A., Akerblad, P., Sigvardsson, M., Rosen, E. D. (2007). Critical Role for Ebf1 and Ebf2 in the Adipogenic Transcriptional Cascade. Mol. Cell. Biol. 27: 743-757 [Abstract] [Full Text]  
  • Akerblad, P., Mansson, R., Lagergren, A., Westerlund, S., Basta, B., Lind, U., Thelin, A., Gisler, R., Liberg, D., Nelander, S., Bamberg, K., Sigvardsson, M. (2005). Gene expression analysis suggests that EBF-1 and PPAR{gamma}2 induce adipogenesis of NIH-3T3 cells with similar efficiency and kinetics. Physiol. Genomics 23: 206-216 [Abstract] [Full Text]  
  • Smith, E. M. K., Akerblad, P., Kadesch, T., Axelson, H., Sigvardsson, M. (2005). Inhibition of EBF function by active Notch signaling reveals a novel regulatory pathway in early B-cell development. Blood 106: 1995-2001 [Abstract] [Full Text]  
  • Mansson, R., Tsapogas, P., Akerlund, M., Lagergren, A., Gisler, R., Sigvardsson, M. (2004). Pearson Correlation Analysis of Microarray Data Allows for the Identification of Genetic Targets for Early B-cell Factor. J. Biol. Chem. 279: 17905-17913 [Abstract] [Full Text]  
  • Liberg, D., Sigvardsson, M., Akerblad, P. (2002). The EBF/Olf/Collier Family of Transcription Factors: Regulators of Differentiation in Cells Originating from All Three Embryonal Germ Layers. Mol. Cell. Biol. 22: 8389-8397 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»