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Molecular and Cellular Biology, December 2002, p. 8669-8680, Vol. 22, No. 24
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.24.8669-8680.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Ash1 Protein, an Asymmetrically Localized Transcriptional Regulator, Controls Filamentous Growth and Virulence of Candida albicans

Diane O. Inglis and Alexander D. Johnson*

Department of Biochemistry and Biophysics and Department of Microbiology and Immunology, University of California—San Francisco, San Francisco, California 94143

Received 5 September 2001/ Returned for modification 26 October 2001/ Accepted 17 September 2002

In response to a number of distinct environmental conditions, the fungal pathogen Candida albicans undergoes a morphological transition from a round, yeast form to a series of elongated, filamentous forms. This transition is believed to be critical for virulence in a mouse model of disseminated candidiasis. Here we describe the characterization of C. albicans ASH1, a gene that encodes an asymmetrically localized transcriptional regulatory protein involved in this response. We show that C. albicans ash1 mutants are defective in responding to some filament-inducing conditions. We also show that Ash1p is preferentially localized to daughter cell nuclei in the budding-yeast form of C. albicans cell growth and to the hyphal tip cells in growing filaments. Thus, Ash1p "marks" newly formed cells and presumably directs a specialized transcriptional program in these cells. Finally, we show that ASH1 is required for full virulence of C. albicans in a mouse model of disseminated candidiasis.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of California—San Francisco, 513 Parnassus Ave., S-410, San Francisco, CA 94143-0414. Phone: (415) 476-8783. Fax: (415) 476-8201. E-mail: ajohnson{at}cgl.ucsf.edu.

Molecular and Cellular Biology, December 2002, p. 8669-8680, Vol. 22, No. 24
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.24.8669-8680.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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