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Molecular and Cellular Biology, February 2002, p. 946-952, Vol. 22, No. 3
0270-7306/01/$04.00+0     DOI: 10.1128/MCB.22.3.946-952.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Essential Role for the Lymphostromal Plasma Membrane Ly-6 Superfamily Molecule Thymic Shared Antigen 1 in Development of the Embryonic Adrenal Gland

David J. Zammit,^1* Stuart P. Berzins,^1, Jason W. Gill,¹ Elise S. Randle-Barrett,¹ Louise Barnett,² Frank Koentgen,^2, Gavin W. Lambert,³ Richard P. Harvey,^4,5 Richard L. Boyd,¹ and Brendan J. Classon¹

Department of Pathology and Immunology, Monash Medical School,¹ Baker Medical Research Institute, Prahran, Victoria 3181,³ The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050,² Victor Chang Cardiac Research Institute, St. Vincent’s Hospital, Darlinghurst, New South Wales 2010,⁴ Departments of Medicine and Life Sciences, University of New South Wales, Kensington, New South Wales 2052, Australia⁵

Received 30 August 2001/ Accepted 30 October 2001

Thymic shared antigen 1 (TSA-1) is a plasma membrane protein of the Ly-6 superfamily expressed on thymocytes, thymic stromal cells, and other cells of the hematopoietic system. TSA-1 is also expressed in other nonhematopoietic tissues, in particular, embryonic and adult adrenal glands. To address the function of TSA-1, we generated mutant mice in which TSA-1 expression was inactivated by gene targeting. Here we show that deletion of both TSA-1 alleles results in abnormal adrenal gland development and midgestational lethality due to cardiac abnormalities. We also report that TSA-1-deficient adrenal glands have significantly reduced levels of the catecholamines noradrenaline and adrenaline. We conclude that TSA-1 is required for normal embryonic development but that deletion of its expression does not obviously impair lymphoid development.

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* Corresponding author. Mailing address: Department of Pathology and Immunology, Monash Medical School, Prahan, Victoria 3181,Australia. Phone: 61 399030896. Fax: 61 399030731. E-mail: david.zammit{at}med.monash.edu.

Present address: Joslin Diabetes Center, Harvard Medical School, Boston, MA 01760.

Present address: Ozgene, Nedlands Campus, Nedlands, Western Australia 6009, Australia.

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Molecular and Cellular Biology, February 2002, p. 946-952, Vol. 22, No. 3
0022-538X/01/$04.00+0     DOI: 10.1128/MCB.22.3.946-952.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

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