This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bertram, P. G.
Right arrow Articles by Zheng, X. F. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bertram, P. G.
Right arrow Articles by Zheng, X. F. S.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, February 2002, p. 1246-1252, Vol. 22, No. 4
0270-7306/01/$04.00+0     DOI: 10.1128/MCB.22.4.1246-1252.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Convergence of TOR-Nitrogen and Snf1-Glucose Signaling Pathways onto Gln3

Paula G. Bertram, Jae H. Choi, John Carvalho, Ting-Fung Chan, Wandong Ai, and X. F. Steven Zheng*

Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110

Received 28 August 2001/ Returned for modification 25 October 2001/ Accepted 13 November 2001

Carbon and nitrogen are two basic nutrient sources for cellular organisms. They supply precursors for energy metabolism and metabolic biosynthesis. In the yeast Saccharomyces cerevisiae, distinct sensing and signaling pathways have been described that regulate gene expression in response to the quality of carbon and nitrogen sources, respectively. Gln3 is a GATA-type transcription factor of nitrogen catabolite-repressible (NCR) genes. Previous observations indicate that the quality of nitrogen sources controls the phosphorylation and cytoplasmic retention of Gln3 via the target of rapamycin (TOR) protein. In this study, we show that glucose also regulates Gln3 phosphorylation and subcellular localization, which is mediated by Snf1, the yeast homolog of AMP-dependent protein kinase and a cytoplasmic glucose sensor. Our data show that glucose and nitrogen signaling pathways converge onto Gln3, which may be critical for both nutrient sensing and starvation responses.

* Corresponding author. Mailing address: Campus Box 8069, Washington University School of Medicine, 660 South Euclid Ave., St. Louis, MO 63110. Phone: (314) 747-1884. Fax: (314) 747-2797. E-mail: zheng{at}pathology.wustl.edu.

Molecular and Cellular Biology, February 2002, p. 1246-1252, Vol. 22, No. 4
0022-538X/01/$04.00+0     DOI: 10.1128/MCB.22.4.1246-1252.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jablonowski, D., Taubert, J.-E., Bar, C., Stark, M. J. R., Schaffrath, R. (2009). Distinct Subsets of Sit4 Holophosphatases Are Required for Inhibition of Saccharomyces cerevisiae Growth by Rapamycin and Zymocin. Eukaryot Cell 8: 1637-1647 [Abstract] [Full Text]  
  • Leverentz, M. K., Campbell, R. N., Connolly, Y., Whetton, A. D., Reece, R. J. (2009). Mutation of a Phosphorylatable Residue in Put3p Affects the Magnitude of Rapamycin-induced PUT1 Activation in a Gat1p-dependent Manner. J. Biol. Chem. 284: 24115-24122 [Abstract] [Full Text]  
  • Wong, K. H., Hynes, M. J., Davis, M. A. (2008). Recent Advances in Nitrogen Regulation: a Comparison between Saccharomyces cerevisiae and Filamentous Fungi. Eukaryot Cell 7: 917-925 [Full Text]  
  • Luzzani, C., Cardillo, S. B., Bermudez Moretti, M., Correa Garcia, S. (2007). New insights into the regulation of the Saccharomyces cerevisiae UGA4 gene: two parallel pathways participate in carbon-regulated transcription. Microbiology 153: 3677-3684 [Abstract] [Full Text]  
  • Mendes-Ferreira, A., del Olmo, M., Garcia-Martinez, J., Jimenez-Marti, E., Mendes-Faia, A., Perez-Ortin, J. E., Leao, C. (2007). Transcriptional Response of Saccharomyces cerevisiae to Different Nitrogen Concentrations during Alcoholic Fermentation. Appl. Environ. Microbiol. 73: 3049-3060 [Abstract] [Full Text]  
  • Orlova, M., Kanter, E., Krakovich, D., Kuchin, S. (2006). Nitrogen Availability and TOR Regulate the Snf1 Protein Kinase in Saccharomyces cerevisiae. Eukaryot Cell 5: 1831-1837 [Abstract] [Full Text]  
  • Garcia-Salcedo, R., Casamayor, A., Ruiz, A., Gonzalez, A., Prista, C., Loureiro-Dias, M. C., Ramos, J., Arino, J. (2006). Heterologous Expression Implicates a GATA Factor in Regulation of Nitrogen Metabolic Genes and Ion Homeostasis in the Halotolerant Yeast Debaryomyces hansenii.. Eukaryot Cell 5: 1388-1398 [Abstract] [Full Text]  
  • Peter, G. J., During, L., Ahmed, A. (2006). Carbon Catabolite Repression Regulates Amino Acid Permeases in Saccharomyces cerevisiae via the TOR Signaling Pathway. J. Biol. Chem. 281: 5546-5552 [Abstract] [Full Text]  
  • Powers, R. W. III, Kaeberlein, M., Caldwell, S. D., Kennedy, B. K., Fields, S. (2006). Extension of chronological life span in yeast by decreased TOR pathway signaling. Genes Dev. 20: 174-184 [Abstract] [Full Text]  
  • Giannattasio, S., Liu, Z., Thornton, J., Butow, R. A. (2005). Retrograde Response to Mitochondrial Dysfunction Is Separable from TOR1/2 Regulation of Retrograde Gene Expression. J. Biol. Chem. 280: 42528-42535 [Abstract] [Full Text]  
  • Todd, R. B., Fraser, J. A., Wong, K. H., Davis, M. A., Hynes, M. J. (2005). Nuclear Accumulation of the GATA Factor AreA in Response to Complete Nitrogen Starvation by Regulation of Nuclear Export. Eukaryot Cell 4: 1646-1653 [Abstract] [Full Text]  
  • Tate, J. J., Rai, R., Cooper, T. G. (2005). Methionine Sulfoximine Treatment and Carbon Starvation Elicit Snf1-independent Phosphorylation of the Transcription Activator Gln3 in Saccharomyces cerevisiae. J. Biol. Chem. 280: 27195-27204 [Abstract] [Full Text]  
  • Shirra, M. K., Rogers, S. E., Alexander, D. E., Arndt, K. M. (2005). The Snf1 Protein Kinase and Sit4 Protein Phosphatase Have Opposing Functions in Regulating TATA-Binding Protein Association With the Saccharomyces cerevisiae INO1 Promoter. Genetics 169: 1957-1972 [Abstract] [Full Text]  
  • Das, M., Bhat, P. J. (2005). Disruption of MRG19 results in altered nitrogen metabolic status and defective pseudohyphal development in Saccharomyces cerevisiae. Microbiology 151: 91-98 [Abstract] [Full Text]  
  • Truckses, D. M., Garrenton, L. S., Thorner, J. (2004). Jekyll and Hyde in the Microbial World. Science 306: 1509-1511 [Abstract] [Full Text]  
  • Gray, J. V., Petsko, G. A., Johnston, G. C., Ringe, D., Singer, R. A., Werner-Washburne, M. (2004). "Sleeping Beauty": Quiescence in Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 68: 187-206 [Abstract] [Full Text]  
  • Cox, K. H., Kulkarni, A., Tate, J. J., Cooper, T. G. (2004). Gln3 Phosphorylation and Intracellular Localization in Nutrient Limitation and Starvation Differ from Those Generated by Rapamycin Inhibition of Tor1/2 in Saccharomyces cerevisiae. J. Biol. Chem. 279: 10270-10278 [Abstract] [Full Text]  
  • Hahn, J.-S., Thiele, D. J. (2004). Activation of the Saccharomyces cerevisiae Heat Shock Transcription Factor Under Glucose Starvation Conditions by Snf1 Protein Kinase. J. Biol. Chem. 279: 5169-5176 [Abstract] [Full Text]  
  • Lee, M., Hwang, J.-T., Lee, H.-J., Jung, S.-N., Kang, I., Chi, S.-G., Kim, S.-S., Ha, J. (2003). AMP-activated Protein Kinase Activity Is Critical for Hypoxia-inducible Factor-1 Transcriptional Activity and Its Target Gene Expression under Hypoxic Conditions in DU145 Cells. J. Biol. Chem. 278: 39653-39661 [Abstract] [Full Text]  
  • Tremblay, J. J., Viger, R. S. (2003). Transcription Factor GATA-4 Is Activated by Phosphorylation of Serine 261 via the cAMP/Protein Kinase A Signaling Pathway in Gonadal Cells. J. Biol. Chem. 278: 22128-22135 [Abstract] [Full Text]  
  • Carvalho, J., Zheng, X. F. S. (2003). Domains of Gln3p Interacting with Karyopherins, Ure2p, and the Target of Rapamycin Protein. J. Biol. Chem. 278: 16878-16886 [Abstract] [Full Text]  
  • Cox, K. H., Tate, J. J., Cooper, T. G. (2002). Cytoplasmic Compartmentation of Gln3 during Nitrogen Catabolite Repression and the Mechanism of Its Nuclear Localization during Carbon Starvation in Saccharomyces cerevisiae. J. Biol. Chem. 277: 37559-37566 [Abstract] [Full Text]  
  • Mayordomo, I., Estruch, F., Sanz, P. (2002). Convergence of the Target of Rapamycin and the Snf1 Protein Kinase Pathways in the Regulation of the Subcellular Localization of Msn2, a Transcriptional Activator of STRE (Stress Response Element)-regulated Genes. J. Biol. Chem. 277: 35650-35656 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»