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Molecular and Cellular Biology, March 2002, p. 1545-1554, Vol. 22, No. 5
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.5.1545-1554.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Loss of the Zymogen Granule Protein Syncollin Affects Pancreatic Protein Synthesis and Transport but Not Secretion

Wolfram Antonin,1 Martin Wagner,2,{dagger} Dietmar Riedel,1 Nils Brose,3 and Reinhard Jahn1*

Departments of Neurobiology,1 Molecular Cell Biology, Max Planck Institute for Biophysical Chemistry 37077 Göttingen,2 Department of Molecular Neurobiology, Max Planck Institute for Experimental Medicine, 37075 Göttingen, Germany3

Received 20 September 2001/ Returned for modification 29 October 2001/ Accepted 4 December 2001

Syncollin is a small protein that is abundantly expressed in pancreatic acinar cells and that is tightly associated with the lumenal side of the zymogen granule membrane. To shed light on the hitherto unknown function of syncollin, we have generated syncollin-deficient mice. The mice are viable and show a normal pancreatic morphology as well as normal release kinetics in response to secretagogue stimulation. Although syncollin is highly enriched in zymogen granules, no change was found in the overall protein content and in the levels of chymotrypsin, trypsin, and amylase. However, syncollin-deficient mice reacted to caerulein hyperstimulation with a more severe pancreatitis. Furthermore, the rates of both protein synthesis and intracellular transport of secretory proteins were reduced. We conclude that syncollin plays a role in maturation and/or concentration of zymogens in zymogen granules.

* Corresponding author. Mailing address: Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany. Phone: 49-551 201 1634. Fax: 49-551 201 1639. E-mail: rjahn{at}gwdg.de.

{dagger} Present address: Department of Gastroenterology and Endocrinology, University of Ulm, 89081 Ulm, Germany.

Molecular and Cellular Biology, March 2002, p. 1545-1554, Vol. 22, No. 5
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.5.1545-1554.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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