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Molecular and Cellular Biology, March 2002, p. 1615-1625, Vol. 22, No. 6
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.6.1615-1625.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Transcription Activator Interactions with Multiple SWI/SNF Subunits

Kristen E. Neely, Ahmed H. Hassan, Christine E. Brown, LeAnn Howe, and Jerry L. Workman*

Howard Hughes Medical Institute and Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802

Received 5 September 2001/ Returned for modification 5 October 2001/ Accepted 4 December 2001

We have previously shown that the yeast SWI/SNF complex stimulates in vitro transcription from chromatin templates in an ATP-dependent manner. SWI/SNF function in this regard requires the presence of an activator with which it can interact directly, linking activator recruitment of SWI/SNF to transcriptional stimulation. In this study, we determine the SWI/SNF subunits that mediate its interaction with activators. Using a photo-cross-linking label transfer strategy, we show that the Snf5, Swi1, and Swi2/Snf2 subunits are contacted by the yeast acidic activators, Gcn4 and Hap4, in the context of the intact native SWI/SNF complex. In addition, we show that the same three subunits can interact individually with acidic activation domains, indicating that each subunit contributes to binding activators. Furthermore, mutations that reduce the activation potential of these activators also diminish its interaction with each of these SWI/SNF subunits. Thus, three distinct subunits of the SWI/SNF complex contribute to its interactions with activation domains.

* Corresponding author. Mailing address: Howard Hughes Medical Institute and Department of Biochemistry and Molecular Biology, 303/306 Althouse Laboratory, The Pennsylvania State University, University Park, PA 16802. Phone: (814) 863-8256. Fax: (814) 863-0099. E-mail: jlw10{at}psu.edu.

Molecular and Cellular Biology, March 2002, p. 1615-1625, Vol. 22, No. 6
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.6.1615-1625.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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