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Molecular and Cellular Biology, April 2002, p. 2047-2056, Vol. 22, No. 7
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.7.2047-2056.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Ime2, a Meiosis-Specific Kinase in Yeast, Is Required for Destabilization of Its Transcriptional Activator, Ime1

Noga Guttmann-Raviv, Sabine Martin, and Yona Kassir*

Department of Biology, Technion, Haifa, 32000 Israel

Received 29 October 2001/ Returned for modification 30 November 2001/ Accepted 17 December 2001

In the budding yeast Saccharomyces cerevisiae, entry into meiosis and its successful completion depend on two positive regulators, Ime1 and Ime2. Ime1 is a transcriptional activator that is required for transcription of IME2, a serine/threonine protein kinase. We show that in vivo Ime2 associates with Ime1, that in vitro Ime2 phosphorylates Ime1, and that in living cells the stability of Ime1 depends on Ime2. Diploid cells with IME2 deleted show an increase in the level of Ime1, whereas haploid cells overexpressing IME2 show a decrease in the stability of Ime1. Furthermore, the level of Ime1 depends on the kinase activity of Ime2. Using a mutation in one of the ATPase subunits of the proteasome, RPT2, we demonstrate that Ime1, amino acids 270 to 360, is degraded by the 26S proteasome. We also show that Ime2 itself is an extremely unstable protein whose expression in vegetative cultures is toxic. We propose that a negative-feedback loop ensures that the activity of Ime1 will be restricted to a narrow window.

* Corresponding author. Mailing address: Department of Biology, Technion, Haifa, 32000 Israel. Phone: 972-4-8294214. Fax: 972-4-8225153. E-mail: ykassir{at}techunix.technion.ac.il.

Molecular and Cellular Biology, April 2002, p. 2047-2056, Vol. 22, No. 7
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.7.2047-2056.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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