This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Morillon, A.
Right arrow Articles by Lesage, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Morillon, A.
Right arrow Articles by Lesage, P.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, April 2002, p. 2078-2088, Vol. 22, No. 7
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.7.2078-2088.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Differential Effects of Chromatin and Gcn4 on the 50-Fold Range of Expression among Individual Yeast Ty1 Retrotransposons

Antonin Morillon, Lionel Bénard, Mathias Springer, and Pascale Lesage*

UPR 9073 du CNRS, Institut de Biologie Physico-Chimique, F-75005 Paris, France

Received 15 November 2001/ Returned for modification 20 December 2001/ Accepted 7 January 2002

Approximately 30 copies of the Ty1 retrotransposon are present in the genome of Saccharomyces cerevisiae. Previous studies gave insights into the global regulation of Ty1 transcription but provided no information on the behavior of individual genomic elements. This work shows that the expression of 31 individual Ty1 elements in S288C varies over a 50-fold range. Their transcription is repressed by chromatin structures, which are antagonized by the Swi/Snf and SAGA chromatin-modifying complexes in highly expressed Ty1 elements. These elements carry five potential Gcn4 binding sites in their promoter regions that are mostly absent in weakly expressed Ty1 copies. Consistent with this observation, Gcn4 activates the transcription of highly expressed Ty1 elements only. One of the potential Gcn4 binding sites acts as an upstream activating sequence in vivo and interacts with Gcn4 in vitro. Since Gcn4 has been shown to interact with Swi/Snf and SAGA, we predict that Gcn4 activates Ty1 transcription by targeting these complexes to specific Ty1 promoters.

* Corresponding author. Mailing address: UPR 9073 du CNRS, Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, F-75005 Paris, France. Phone: (33) 1 58 41 51 25. Fax: (33) 1 58 41 50 20. E-mail: lesage{at}ibpc.fr.

Molecular and Cellular Biology, April 2002, p. 2078-2088, Vol. 22, No. 7
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.7.2078-2088.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Matsuda, E., Garfinkel, D. J. (2009). Posttranslational interference of Ty1 retrotransposition by antisense RNAs. Proc. Natl. Acad. Sci. USA 106: 15657-15662 [Abstract] [Full Text]  
  • Servant, G., Pennetier, C., Lesage, P. (2008). Remodeling Yeast Gene Transcription by Activating the Ty1 Long Terminal Repeat Retrotransposon under Severe Adenine Deficiency. Mol. Cell. Biol. 28: 5543-5554 [Abstract] [Full Text]  
  • Rutherford, J. C., Chua, G., Hughes, T., Cardenas, M. E., Heitman, J. (2008). A Mep2-dependent Transcriptional Profile Links Permease Function to Gene Expression during Pseudohyphal Growth in Saccharomyces cerevisiae. Mol. Biol. Cell 19: 3028-3039 [Abstract] [Full Text]  
  • Berretta, J., Pinskaya, M., Morillon, A. (2008). A cryptic unstable transcript mediates transcriptional trans-silencing of the Ty1 retrotransposon in S. cerevisiae. Genes Dev. 22: 615-626 [Abstract] [Full Text]  
  • Nyswaner, K. M., Checkley, M. A., Yi, M., Stephens, R. M., Garfinkel, D. J. (2008). Chromatin-Associated Genes Protect the Yeast Genome From Ty1 Insertional Mutagenesis. Genetics 178: 197-214 [Abstract] [Full Text]  
  • Todeschini, A.-L., Morillon, A., Springer, M., Lesage, P. (2005). Severe Adenine Starvation Activates Ty1 Transcription and Retrotransposition in Saccharomyces cerevisiae. Mol. Cell. Biol. 25: 7459-7472 [Abstract] [Full Text]  
  • Gelbart, M. E., Bachman, N., Delrow, J., Boeke, J. D., Tsukiyama, T. (2005). Genome-wide identification of Isw2 chromatin-remodeling targets by localization of a catalytically inactive mutant. Genes Dev. 19: 942-954 [Abstract] [Full Text]  
  • Radford, S. J., Boyle, M. L., Sheely, C. J., Graham, J., Haeusser, D. P., Zimmerman, L., Keeney, J. B. (2004). Increase in Ty1 cDNA Recombination in Yeast sir4 Mutant Strains at High Temperature. Genetics 168: 89-101 [Abstract] [Full Text]  
  • Bachman, N., Eby, Y., Boeke, J. D. (2004). Local Definition of Ty1 Target Preference by Long Terminal Repeats and Clustered tRNA Genes. Genome Res 14: 1232-1247 [Abstract] [Full Text]  
  • Dziak, R., Leishman, D., Radovic, M., Tye, B. K., Yankulov, K. (2003). Evidence for a Role of MCM (Mini-chromosome Maintenance)5 in Transcriptional Repression of Sub-telomeric and Ty-proximal Genes in Saccharomyces cerevisiae. J. Biol. Chem. 278: 27372-27381 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»