This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pulvermüller, A.
Right arrow Articles by Wolfrum, U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pulvermüller, A.
Right arrow Articles by Wolfrum, U.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, April 2002, p. 2194-2203, Vol. 22, No. 7
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.7.2194-2203.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Calcium-Dependent Assembly of Centrin-G-Protein Complex in Photoreceptor Cells

Alexander Pulvermüller,1* Andreas Gießl,2 Martin Heck,1 Ralf Wottrich,2,{dagger} Angelika Schmitt,2 Oliver Peter Ernst,1 Hui-Woog Choe,1,3 Klaus Peter Hofmann,1 and Uwe Wolfrum2*

Institut für Medizinische Physik und Biophysik, Humboldt-Universität zu Berlin, Universitätsklinikum Charité, 10098 Berlin ,1 Institut für Zoologie, Johannes Gutenberg-Universität Mainz, 55099 Mainz, Germany ,2 Department of Chemistry, Chonbuk National University, Chonju, South Korea3

Received 25 June 2001/ Returned for modification 3 August 2001/ Accepted 17 December 2001

Photoexcitation of rhodopsin activates a heterotrimeric G-protein cascade leading to cyclic GMP hydrolysis in vertebrate photoreceptors. Light-induced exchanges of the visual G-protein transducin between the outer and inner segment of rod photoreceptors occur through the narrow connecting cilium. Here we demonstrate that transducin colocalizes with the Ca2+-binding protein centrin 1 in a specific domain of this cilium. Coimmunoprecipitation, centrifugation, centrin overlay, size exclusion chromatography, and kinetic light-scattering experiments indicate that Ca2+-activated centrin 1 binds with high affinity and specificity to transducin. The assembly of centrin-G-protein complex is mediated by the ß{gamma}-complex. The Ca2+-dependent assembly of a G protein with centrin is a novel aspect of the supply of signaling proteins in sensory cells and a potential link between molecular translocations and signal transduction in general.

* Corresponding author. Mailing address for Alexander Pulvermüller: Institut für Medizinische Physik und Biophysik, Charité, Medizinische Fakultät der Humboldt-Universität zu Berlin, Ziegelstrasse 5-9, D-10117 Berlin, Germany. Phone: 49 (0)30 450 524176. Fax: 49 (0)30 450 524952. E-mail: alexander.pulvermueller{at}charite.de. Mailing address for Uwe Wolfrum: Institut für Zoologie, Abt. 1, Johannes Gutenberg-Universität Mainz, Müllerweg 6, D-55099 Mainz, Germany. Phone: 49 (0)6131 39 25148. Fax: 49 (0)6131 39 23815. E-mail: wolfrum{at}mail.uni-mainz.de.

{dagger} Present address: ITOX, Forschungszentrum Karlsruhe, 76128 Karlsruhe, Germany.

Molecular and Cellular Biology, April 2002, p. 2194-2203, Vol. 22, No. 7
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.7.2194-2203.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lobanova, E. S., Finkelstein, S., Song, H., Tsang, S. H., Chen, C.-K., Sokolov, M., Skiba, N. P., Arshavsky, V. Y. (2007). Transducin Translocation in Rods Is Triggered by Saturation of the GTPase-Activating Complex. J. Neurosci. 27: 1151-1160 [Abstract] [Full Text]  
  • Thompson, J. R., Ryan, Z. C., Salisbury, J. L., Kumar, R. (2006). The Structure of the Human Centrin 2-Xeroderma Pigmentosum Group C Protein Complex. J. Biol. Chem. 281: 18746-18752 [Abstract] [Full Text]  
  • Strissel, K. J., Sokolov, M., Trieu, L. H., Arshavsky, V. Y. (2006). Arrestin Translocation Is Induced at a Critical Threshold of Visual Signaling and Is Superstoichiometric to Bleached Rhodopsin. J. Neurosci. 26: 1146-1153 [Abstract] [Full Text]  
  • Steele, E. C. Jr., Chen, X., Iuvone, P. M., MacLeish, P. R. (2005). Imaging of Ca2+ Dynamics Within the Presynaptic Terminals of Salamander Rod Photoreceptors. J. Neurophysiol. 94: 4544-4553 [Abstract] [Full Text]  
  • Peterson, J. J., Orisme, W., Fellows, J., McDowell, J. H., Shelamer, C. L., Dugger, D. R., Smith, W. C. (2005). A Role for Cytoskeletal Elements in the Light-Driven Translocation of Proteins in Rod Photoreceptors. IOVS 46: 3988-3998 [Abstract] [Full Text]  
  • Geimer, S., Melkonian, M. (2005). Centrin Scaffold in Chlamydomonas reinhardtii Revealed by Immunoelectron Microscopy. Eukaryot Cell 4: 1253-1263 [Abstract] [Full Text]  
  • Shu, X., Fry, A.M., Tulloch, B., Manson, F.D.C., Crabb, J.W., Khanna, H., Faragher, A.J., Lennon, A., He, S., Trojan, P., Giessl, A., Wolfrum, U., Vervoort, R., Swaroop, A., Wright, A.F. (2005). RPGR ORF15 isoform co-localizes with RPGRIP1 at centrioles and basal bodies and interacts with nucleophosmin. Hum Mol Genet 14: 1183-1197 [Abstract] [Full Text]  
  • Giessl, A., Pulvermuller, A., Trojan, P., Park, J. H., Choe, H.-W., Ernst, O. P., Hofmann, K. P., Wolfrum, U. (2004). Differential Expression and Interaction with the Visual G-protein Transducin of Centrin Isoforms in Mammalian Photoreceptor Cells. J. Biol. Chem. 279: 51472-51481 [Abstract] [Full Text]  
  • Senin, I. I., Hoppner-Heitmann, D., Polkovnikova, O. O., Churumova, V. A., Tikhomirova, N. K., Philippov, P. P., Koch, K.-W. (2004). Recoverin and Rhodopsin Kinase Activity in Detergent-resistant Membrane Rafts from Rod Outer Segments. J. Biol. Chem. 279: 48647-48653 [Abstract] [Full Text]  
  • Zimmermann, K., Ritter, E., Bartl, F. J., Hofmann, K. P., Heck, M. (2004). Interaction with Transducin Depletes Metarhodopsin III: A REGULATED RETINAL STORAGE IN VISUAL SIGNAL TRANSDUCTION?. J. Biol. Chem. 279: 48112-48119 [Abstract] [Full Text]  
  • Peet, J. A., Bragin, A., Calvert, P. D., Nikonov, S. S., Mani, S., Zhao, X., Besharse, J. C., Pierce, E. A., Knox, B. E., Pugh, E. N. Jr (2004). Quantification of the cytoplasmic spaces of living cells with EGFP reveals arrestin-EGFP to be in disequilibrium in dark adapted rod photoreceptors. J. Cell Sci. 117: 3049-3059 [Abstract] [Full Text]  
  • Sokolov, M., Strissel, K. J., Leskov, I. B., Michaud, N. A., Govardovskii, V. I., Arshavsky, V. Y. (2004). Phosducin Facilitates Light-driven Transducin Translocation in Rod Photoreceptors: EVIDENCE FROM THE PHOSDUCIN KNOCKOUT MOUSE. J. Biol. Chem. 279: 19149-19156 [Abstract] [Full Text]  
  • Arshavsky, V. Y. (2003). Protein Translocation in Photoreceptor Light Adaptation: A Common Theme in Vertebrate and Invertebrate Vision. Sci Signal 2003: pe43-pe43 [Abstract] [Full Text]  
  • Kilmartin, J. V. (2003). Sfi1p has conserved centrin-binding sites and an essential function in budding yeast spindle pole body duplication. JCB 162: 1211-1221 [Abstract] [Full Text]  
  • Gavet, O., Alvarez, C., Gaspar, P., Bornens, M. (2003). Centrin4p, a Novel Mammalian Centrin Specifically Expressed in Ciliated Cells. Mol. Biol. Cell 14: 1818-1834 [Abstract] [Full Text]  
  • Schmidt, M., Giessl, A., Laufs, T., Hankeln, T., Wolfrum, U., Burmester, T. (2003). How Does the Eye Breathe? EVIDENCE FOR NEUROGLOBIN-MEDIATED OXYGEN SUPPLY IN THE MAMMALIAN RETINA. J. Biol. Chem. 278: 1932-1935 [Abstract] [Full Text]  
  • Schroder, K., Pulvermuller, A., Hofmann, K. P. (2002). Arrestin and Its Splice Variant Arr1-370A (p44). MECHANISM AND BIOLOGICAL ROLE OF THEIR INTERACTION WITH RHODOPSIN. J. Biol. Chem. 277: 43987-43996 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»