This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Livengood, A. J.
Right arrow Articles by Cech, T. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Livengood, A. J.
Right arrow Articles by Cech, T. R.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, April 2002, p. 2366-2374, Vol. 22, No. 7
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.7.2366-2374.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Essential Regions of Saccharomyces cerevisiae Telomerase RNA: Separate Elements for Est1p and Est2p Interaction

April J. Livengood,1 Arthur J. Zaug,2,3 and Thomas R. Cech1,2,3*

Department of MCD Biology,1 Department of Chemistry and Biochemistry,2 Howard Hughes Medical Institute, University of Colorado, Boulder, Colorado 80309-02153

Received 20 September 2001/ Returned for modification 1 November 2001/ Accepted 3 January 2002

The Saccharomyces cerevisiae telomerase RNA subunit is encoded by the TLC1 gene. A selection for viable alleles of TLC1 RNA from a large library of random deletion alleles revealed that less than half (~0.5 kb of the ~1.3-kb RNA) is required for telomerase function in vivo. The main essential region (430 nucleotides), which contains the template for telomeric DNA synthesis, was required for coimmunoprecipitation with Est1p and Est2p. Furthermore, the subregion required for interaction with Est1p, the telomerase recruitment subunit, differed from those required for interaction with Est2p, the reverse transcriptase subunit. Two regions of the RNA distant from the template in the nucleotide sequence were required for Est2p binding, but the template itself was not. Having the RNA secured to the protein away from the template is proposed to facilitate the translocation of the RNA template through the active site. More generally, our results support a role for the telomerase RNA serving as a scaffold for binding key protein subunits.


* Corresponding author. Mailing address: Howard Hughes Medical Institute, 4000 Jones Bridge Rd., Chevy Chase, MD 20815. Phone: (301) 215-8550. Fax: (301) 215-8558. E-mail: CECHT{at}hhmi.org.


Molecular and Cellular Biology, April 2002, p. 2366-2374, Vol. 22, No. 7
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.7.2366-2374.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.




This article has been cited by other articles:

  • DeZwaan, D. C., Freeman, B. C. (2009). The conserved Est1 protein stimulates telomerase DNA extension activity. Proc. Natl. Acad. Sci. USA 106: 17337-17342 [Abstract] [Full Text]  
  • Gunisova, S., Elboher, E., Nosek, J., Gorkovoy, V., Brown, Y., Lucier, J.-F., Laterreur, N., Wellinger, R. J., Tzfati, Y., Tomaska, L. (2009). Identification and comparative analysis of telomerase RNAs from Candida species reveal conservation of functional elements. RNA 15: 546-559 [Abstract] [Full Text]  
  • Franke, J., Gehlen, J., Ehrenhofer-Murray, A. E. (2008). Hypermethylation of yeast telomerase RNA by the snRNA and snoRNA methyltransferase Tgs1. J. Cell Sci. 121: 3553-3560 [Abstract] [Full Text]  
  • Errington, T. M., Fu, D., Wong, J. M. Y., Collins, K. (2008). Disease-Associated Human Telomerase RNA Variants Show Loss of Function for Telomere Synthesis without Dominant-Negative Interference. Mol. Cell. Biol. 28: 6510-6520 [Abstract] [Full Text]  
  • Brown, Y., Abraham, M., Pearl, S., Kabaha, M. M., Elboher, E., Tzfati, Y. (2007). A critical three-way junction is conserved in budding yeast and vertebrate telomerase RNAs. Nucleic Acids Res 35: 6280-6289 [Abstract] [Full Text]  
  • Shefer, K., Brown, Y., Gorkovoy, V., Nussbaum, T., Ulyanov, N. B., Tzfati, Y. (2007). A Triple Helix within a Pseudoknot Is a Conserved and Essential Element of Telomerase RNA. Mol. Cell. Biol. 27: 2130-2143 [Abstract] [Full Text]  
  • Mozdy, A. D., Cech, T. R. (2006). Low abundance of telomerase in yeast: Implications for telomerase haploinsufficiency. RNA 12: 1721-1737 [Abstract] [Full Text]  
  • Yang, C.-P., Chen, Y.-B., Meng, F.-L., Zhou, J.-Q. (2006). Saccharomyces cerevisiae Est3p dimerizes in vitro and dimerization contributes to efficient telomere replication in vivo. Nucleic Acids Res 34: 407-416 [Abstract] [Full Text]  
  • ZAPPULLA, D.C., CECH, T.R. (2006). RNA as a Flexible Scaffold for Proteins: Yeast Telomerase and Beyond. Cold Spring Harb Symp Quant Biol 71: 217-224 [Abstract]  
  • Chen, J.-L., Greider, C. W. (2004). An emerging consensus for telomerase RNA structure. Proc. Natl. Acad. Sci. USA 101: 14683-14684 [Full Text]  
  • Lin, J., Ly, H., Hussain, A., Abraham, M., Pearl, S., Tzfati, Y., Parslow, T. G., Blackburn, E. H. (2004). From the Cover: A universal telomerase RNA core structure includes structured motifs required for binding the telomerase reverse transcriptase protein. Proc. Natl. Acad. Sci. USA 101: 14713-14718 [Abstract] [Full Text]  
  • Chappell, A. S., Lundblad, V. (2004). Structural Elements Required for Association of the Saccharomyces cerevisiae Telomerase RNA with the Est2 Reverse Transcriptase. Mol. Cell. Biol. 24: 7720-7736 [Abstract] [Full Text]  
  • Zappulla, D. C., Cech, T. R. (2004). From The Cover: Yeast telomerase RNA: A flexible scaffold for protein subunits. Proc. Natl. Acad. Sci. USA 101: 10024-10029 [Abstract] [Full Text]  
  • Bryan, T. M., Goodrich, K. J., Cech, T. R. (2003). Tetrahymena Telomerase Is Active as a Monomer. Mol. Biol. Cell 14: 4794-4804 [Abstract] [Full Text]  
  • SETO, A. G., UMANSKY, K., TZFATI, Y., ZAUG, A. J., BLACKBURN, E. H., CECH, T. R. (2003). A template-proximal RNA paired element contributes to Saccharomyces cerevisiae telomerase activity. RNA 9: 1323-1332 [Abstract] [Full Text]  
  • Tzfati, Y., Knight, Z., Roy, J., Blackburn, E. H. (2003). A novel pseudoknot element is essential for the action of a yeast telomerase. Genes Dev. 17: 1779-1788 [Abstract] [Full Text]  
  • Forstemann, K., Zaug, A. J., Cech, T. R., Lingner, J. (2003). Yeast telomerase is specialized for C/A-rich RNA templates. Nucleic Acids Res 31: 1646-1655 [Abstract] [Full Text]  
  • Friedman, K. L., Heit, J. J., Long, D. M., Cech, T. R. (2003). N-terminal Domain of Yeast Telomerase Reverse Transcriptase: Recruitment of Est3p to the Telomerase Complex. Mol. Biol. Cell 14: 1-13 [Abstract] [Full Text]  
  • Seto, A. G., Livengood, A. J., Tzfati, Y., Blackburn, E. H., Cech, T. R. (2002). A bulged stem tethers Est1p to telomerase RNA in budding yeast. Genes Dev. 16: 2800-2812 [Abstract] [Full Text]  
  • Evans, S. K., Lundblad, V. (2002). The Est1 Subunit of Saccharomyces cerevisiae Telomerase Makes Multiple Contributions to Telomere Length Maintenance. Genetics 162: 1101-1115 [Abstract] [Full Text]