This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Intano, G. W.
Right arrow Articles by Walter, C. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Intano, G. W.
Right arrow Articles by Walter, C. A.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, April 2002, p. 2410-2418, Vol. 22, No. 7
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.7.2410-2418.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Base Excision Repair Is Limited by Different Proteins in Male Germ Cell Nuclear Extracts Prepared from Young and Old Mice

Gabriel W. Intano,1 C. Alex McMahan,2 John R. McCarrey,3 Ronald B. Walter,4 Allison E. McKenna,5 Yoshihiro Matsumoto,5 Mark A. MacInnes,6 David J. Chen,7 and Christi A. Walter1,8*

Department of Cellular & Structural Biology,1 Department of Pathology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229-3900,2 South Texas Veterans Health Care System, Audie L. Murphy Hospital, San Antonio, Texas 78284,8 Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, Texas 78245,3 Department of Chemistry and Biochemistry, Southwest Texas State University, San Marcos, Texas 78666,4 Department of Radiation Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111,5 Biosciences Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545,6 Life Science Division, Lawrence Berkeley National Laboratory, Berkeley, California 947207

Received 23 July 2001/ Returned for modification 4 October 2001/ Accepted 20 December 2001

The combined observations of elevated DNA repair gene expression, high uracil-DNA glycosylase-initiated base excision repair, and a low spontaneous mutant frequency for a lacI transgene in spermatogenic cells from young mice suggest that base excision repair activity is high in spermatogenic cell types. Notably, the spontaneous mutant frequency of the lacI transgene is greater in spermatogenic cells obtained from old mice, suggesting that germ line DNA repair activity may decline with age. A paternal age effect in spermatogenic cells is recognized for the human population as well. To determine if male germ cell base excision repair activity changes with age, uracil-DNA glycosylase-initiated base excision repair activity was measured in mixed germ cell (i.e., all spermatogenic cell types in adult testis) nuclear extracts prepared from young, middle-aged, and old mice. Base excision repair activity was also assessed in nuclear extracts from premeiotic, meiotic, and postmeiotic spermatogenic cell types obtained from young mice. Mixed germ cell nuclear extracts exhibited an age-related decrease in base excision repair activity that was restored by addition of apurinic/apyrimidinic (AP) endonuclease. Uracil-DNA glycosylase and DNA ligase were determined to be limiting in mixed germ cell nuclear extracts prepared from young animals. Base excision repair activity was only modestly elevated in pachytene spermatocytes and round spermatids relative to other spermatogenic cells. Thus, germ line short-patch base excision repair activity appears to be relatively constant throughout spermatogenesis in young animals, limited by uracil-DNA glycosylase and DNA ligase in young animals, and limited by AP endonuclease in old animals.

* Corresponding author. Mailing address: Department of Cellular and Structural Biology, Mail Code 7762, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78284-3900. Phone: (210) 567-3832. Fax: (210) 567-3803. E-mail: walter{at}uthscsa.edu.

Molecular and Cellular Biology, April 2002, p. 2410-2418, Vol. 22, No. 7
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.7.2410-2418.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Forand, A., Fouchet, P., Lahaye, J.-B., Chicheportiche, A., Habert, R., Bernardino-Sgherri, J. (2009). Similarities and Differences in the In Vivo Response of Mouse Neonatal Gonocytes and Spermatogonia to Genotoxic Stress. Biol. Reprod. 80: 860-873 [Abstract] [Full Text]  
  • Allen, D., Herbert, D. C., McMahan, C. A., Rotrekl, V., Sobol, R. W., Wilson, S. H., Walter, C. A. (2008). Mutagenesis Is Elevated in Male Germ Cells Obtained from DNA Polymerase-beta Heterozygous Mice. Biol. Reprod. 79: 824-831 [Abstract] [Full Text]  
  • Johnston, D. S., Wright, W. W., DiCandeloro, P., Wilson, E., Kopf, G. S., Jelinsky, S. A. (2008). Stage-specific gene expression is a fundamental characteristic of rat spermatogenic cells and Sertoli cells. Proc. Natl. Acad. Sci. USA 105: 8315-8320 [Abstract] [Full Text]  
  • Chang, I.-Y., Jin, M., Yoon, S. P., Youn, C.-K., Yoon, Y., Moon, S.-P., Hyun, J.-W., Jun, J. Y., You, H. J. (2008). Senescence-Dependent MutS{alpha} Dysfunction Attenuates Mismatch Repair. Mol Cancer Res 6: 978-989 [Abstract] [Full Text]  
  • Gorbunova, V., Seluanov, A., Mao, Z., Hine, C. (2007). Changes in DNA repair during aging. Nucleic Acids Res 35: 7466-7474 [Abstract] [Full Text]  
  • Evans, D G R, Maher, E R, Baser, M E (2005). Age related shift in the mutation spectra of germline and somatic NF2 mutations: hypothetical role of DNA repair mechanisms. J. Med. Genet. 42: 630-632 [Abstract] [Full Text]  
  • Xu, G., Spivak, G., Mitchell, D. L., Mori, T., McCarrey, J. R., McMahan, C. A., Walter, R. B., Hanawalt, P. C., Walter, C. A. (2005). Nucleotide Excision Repair Activity Varies Among Murine Spermatogenic Cell Types. Biol. Reprod. 73: 123-130 [Abstract] [Full Text]  
  • Huamani, J., McMahan, C. A., Herbert, D. C., Reddick, R., McCarrey, J. R., MacInnes, M. I., Chen, D. J., Walter, C. A. (2004). Spontaneous Mutagenesis Is Enhanced in Apex Heterozygous Mice. Mol. Cell. Biol. 24: 8145-8153 [Abstract] [Full Text]  
  • Seluanov, A., Mittelman, D., Pereira-Smith, O. M., Wilson, J. H., Gorbunova, V. (2004). DNA end joining becomes less efficient and more error-prone during cellular senescence. Proc. Natl. Acad. Sci. USA 101: 7624-7629 [Abstract] [Full Text]  
  • Intano, G. W., Cho, E. J., McMahan, C. A., Walter, C. A. (2003). Age-related Base Excision Repair Activity in Mouse Brain and Liver Nuclear Extracts. Journals of Gerontology Series A: Biological Sciences and Medical Sciences 58: B205-211 [Abstract] [Full Text]  
  • Walter, C. A., Walter, R. B., McCarrey, J. R. (2003). Germline Genomes--A Biological Fountain of Youth?. Sci Aging Knowl Environ 2003: pe4-4 [Abstract] [Full Text]  
  • Olsen, A.-K., Duale, N., Bjoras, M., Larsen, C. T., Wiger, R., Holme, J. A., Seeberg, E. C., Brunborg, G. (2003). Limited repair of 8-hydroxy-7,8-dihydroguanine residues in human testicular cells. Nucleic Acids Res 31: 1351-1363 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»