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 Previous Article

Molecular and Cellular Biology, January 2003, p. 414-423, Vol. 23, No. 1
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.1.414-423.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

A Novel Domain within the DEAD-Box Protein DP103 Is Essential for Transcriptional Repression and Helicase Activity

Xiaomei Yan, Jean-François Mouillet, Qinglin Ou, and Yoel Sadovsky*

Department of Obstetrics and Gynecology and Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110

Received 25 February 2002/ Returned for modification 22 April 2002/ Accepted 26 September 2002

Members of the DEAD-box family of helicases, distinguished by a core characteristic sequence of Asp-Glu-Ala-Asp, are expressed in a wide range of prokaryotes and eukaryotes and exhibit diverse cellular functions, including DNA transcription, recombination and repair, RNA processing, translation, and posttranslational regulation. Although ubiquitous, the function of most DEAD-box proteins is unknown. We and others have recently cloned DP103, which harbors conserved DEAD-box, helicase, and ATPase domains in its N terminus. DP103 (also termed Gemin3 and DDX20) interacts with SF-1, SMN, EBNA2, and EBNA3C in mammalian cells. Here we demonstrate that a discrete domain within the nonconserved C-terminal region of DP103 directly interacts with SF-1. This domain exhibits an autonomous repression function and is necessary and sufficient for repressing the transcriptional activity of SF-1. Furthermore, intact DP103 exhibits helicase activity. Importantly, the C-terminal domain is obligatory but not sufficient for this unwinding activity of DP103. Together, our results support a novel paradigm for transcriptional repression and demonstrate the bifunctional role of the C-terminal domain of DP103.

* Corresponding author. Mailing address: Department of Obstetrics and Gynecology, Washington University School of Medicine, 4566 Scott Ave., Campus Box 8064, St. Louis, MO 63110. Phone: (314) 747-0937. Fax: (314) 747-1256. E-mail: sadovskyy{at}msnotes.wustl.edu.

Molecular and Cellular Biology, January 2003, p. 414-423, Vol. 23, No. 1
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.1.414-423.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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