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Molecular and Cellular Biology, July 2003, p. 4859-4869, Vol. 23, No. 14
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.14.4859-4869.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

MAQ1 and 7SK RNA Interact with CDK9/Cyclin T Complexes in a Transcription-Dependent Manner

Annemieke A. Michels,1 Van Trung Nguyen,1 Alessandro Fraldi,2 Valérie Labas,3 Mia Edwards,1 François Bonnet,1 Luigi Lania,2 and Olivier Bensaude1*

UMR 8541 CNRS, Ecole Normale Supérieure, Laboratoire de Régulation de l'Expression Génétique, 75230 Paris Cedex 05,1 UMR 7637 CNRS, Ecole Supérieure de Physique et Chimie Industrielles, 75005 Paris, France,3 Dipartimento di Genetica, Biologia Generale e Molecolare, Università di Napoli "Federico II," 80134 Naples, Italy2

Received 21 February 2003/ Returned for modification 8 April 2003/ Accepted 24 April 2003

Positive transcription elongation factor b (P-TEFb) comprises a cyclin (T1 or T2) and a kinase, cyclin-dependent kinase 9 (CDK9), which phosphorylates the carboxyl-terminal domain of RNA polymerase II. P-TEFb is essential for transcriptional elongation in human cells. A highly specific interaction among cyclin T1, the viral protein Tat, and the transactivation response (TAR) element RNA determines the productive transcription of the human immunodeficiency virus genome. In growing HeLa cells, half of P-TEFb is kinase inactive and binds to the 7SK small nuclear RNA. We now report on a novel protein termed MAQ1 (for ménage à quatre) that is also present in this complex. Since 7SK RNA is required for MAQ1 to associate with P-TEFb, a structural role for 7SK RNA is proposed. Inhibition of transcription results in the release of both MAQ1 and 7SK RNA from P-TEFb. Thus, MAQ1 cooperates with 7SK RNA to form a novel type of CDK inhibitor. According to yeast two-hybrid analysis and immunoprecipitations from extracts of transfected cells, MAQ1 binds directly to the N-terminal cyclin homology region of cyclins T1 and T2. Since Tat also binds to this cyclin T1 N-terminal domain and since the association between 7SK RNA/MAQ1 and P-TEFb competes with the binding of Tat to cyclin T1, we speculate that the TAR RNA/Tat lentivirus system has evolved to subvert the cellular 7SK RNA/MAQ1 system.

* Corresponding author. Mailing address: Ecole Normale Supérieure, Laboratoire de Régulation de l'Expression Génétique, 46 rue d'Ulm, 75230 Paris Cedex 05, France. Phone: (33) 1 4432 3410. Fax: (33) 1 4432 3941. E-mail: bensaude{at}wotan.ens.fr.

Molecular and Cellular Biology, July 2003, p. 4859-4869, Vol. 23, No. 14
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.14.4859-4869.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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