This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kennell, J. A.
Right arrow Articles by MacDougald, O. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kennell, J. A.
Right arrow Articles by MacDougald, O. A.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, August 2003, p. 5366-5375, Vol. 23, No. 15
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.15.5366-5375.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

T-Cell Factor 4N (TCF-4N), a Novel Isoform of Mouse TCF-4, Synergizes with ß-Catenin To Coactivate C/EBP{alpha} and Steroidogenic Factor 1 Transcription Factors

Jennifer A. Kennell,1 Erin E. O'Leary,1 Brian M. Gummow,2 Gary D. Hammer,2,3 and Ormond A. MacDougald1,2*

Program in Cellular and Molecular Biology,1 Departments of Molecular and Integrative Physiology,2 Internal Medicine, Division of Endocrinology and Metabolism, University of Michigan Medical School, Ann Arbor, Michigan 48109-06223

Received 26 March 2003/ Returned for modification 5 May 2003/ Accepted 9 May 2003

We have cloned T-cell factor 4N (TCF-4N), an alternative isoform of TCF-4, from developing pituitary and 3T3-L1 preadipocytes. This protein contains the N-terminal interaction domain for ß-catenin but lacks the DNA binding domain. While TCF-4N inhibited coactivation by ß-catenin of a TCF/lymphoid-enhancing factor (LEF)-dependent promoter, TCF-4N potentiated coactivation by ß-catenin of several non-TCF/LEF-dependent promoters. For example, TCF-4N synergized with ß-catenin to activate the {alpha}-inhibin promoter through functional and physical interactions with the orphan nuclear receptor steroidogenic factor 1 (SF-1). In addition, TCF-4N and ß-catenin synergized with the adipogenic transcription factor CCAAT/enhancer binding protein {alpha} (C/EBP{alpha}) to induce leptin promoter activity. The mechanism by which ß-catenin and TCF-4N coactivated C/EBP{alpha} appeared to involve p300, based upon synergy between these important transcriptional regulators. Consistent with TCF-4N's redirecting the actions of ß-catenin in cells, ectopic expression of TCF-4N in 3T3-L1 preadipocytes partially relieved the block of adipogenesis caused by ß-catenin. Thus, we propose that TCF-4N inhibits coactivation by ß-catenin of TCF/LEF transcription factors and potentiates the coactivation by ß-catenin of other transcription factors, such as SF-1 and C/EBP{alpha}.

* Corresponding author. Mailing address: Department of Physiology, University of Michigan Medical School, 1301 E. Catherine Rd., Ann Arbor, MI 48109-0622. Phone: (734) 647-4880. Fax: (734) 936-8813. E-mail: macdouga{at}umich.edu.

Molecular and Cellular Biology, August 2003, p. 5366-5375, Vol. 23, No. 15
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.15.5366-5375.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hausman, G. J., Dodson, M. V., Ajuwon, K., Azain, M., Barnes, K. M., Guan, L. L., Jiang, Z., Poulos, S. P., Sainz, R. D., Smith, S., Spurlock, M., Novakofski, J., Fernyhough, M. E., Bergen, W. G. (2009). BOARD-INVITED REVIEW: The biology and regulation of preadipocytes and adipocytes in meat animals. J ANIM SCI 87: 1218-1246 [Abstract] [Full Text]  
  • Salisbury, T. B., Binder, A. K., Grammer, J. C., Nilson, J. H. (2009). GnRH-Regulated Expression of Jun and JUN Target Genes in Gonadotropes Requires a Functional Interaction between TCF/LEF Family Members and {beta}-Catenin. Mol. Endocrinol. 23: 402-411 [Abstract] [Full Text]  
  • Park, K. W., Waki, H., Villanueva, C. J., Monticelli, L. A., Hong, C., Kang, S., MacDougald, O. A., Goldrath, A. W., Tontonoz, P. (2008). Inhibitor of DNA Binding 2 Is a Small Molecule-Inducible Modulator of Peroxisome Proliferator-Activated Receptor-{gamma} Expression and Adipocyte Differentiation. Mol. Endocrinol. 22: 2038-2048 [Abstract] [Full Text]  
  • Brennan, C. H, Chittka, A., Barker, S., Vinson, G. P (2008). Eph receptors and zonation in the rat adrenal cortex. J Endocrinol 198: 185-191 [Abstract] [Full Text]  
  • Kang, S., Bennett, C. N., Gerin, I., Rapp, L. A., Hankenson, K. D., MacDougald, O. A. (2007). Wnt Signaling Stimulates Osteoblastogenesis of Mesenchymal Precursors by Suppressing CCAAT/Enhancer-binding Protein {alpha} and Peroxisome Proliferator-activated Receptor {gamma}. J. Biol. Chem. 282: 14515-14524 [Abstract] [Full Text]  
  • Jimenez, M. A., Akerblad, P., Sigvardsson, M., Rosen, E. D. (2007). Critical Role for Ebf1 and Ebf2 in the Adipogenic Transcriptional Cascade. Mol. Cell. Biol. 27: 743-757 [Abstract] [Full Text]  
  • Mellon, S. H., Bair, S. R., Depoix, C., Vigne, J.-L., Hecht, N. B., Brake, P. B. (2007). Translin Coactivates Steroidogenic Factor-1-Stimulated Transcription. Mol. Endocrinol. 21: 89-105 [Abstract] [Full Text]  
  • Brown, A. L., Wilkinson, C. R., Waterman, S. R., Kok, C. H., Salerno, D. G., Diakiw, S. M., Reynolds, B., Scott, H. S., Tsykin, A., Glonek, G. F., Goodall, G. J., Solomon, P. J., Gonda, T. J., D'Andrea, R. J. (2006). Genetic regulators of myelopoiesis and leukemic signaling identified by gene profiling and linear modeling. J. Leukoc. Biol. 80: 433-447 [Abstract] [Full Text]  
  • Xu, W., Fukuyama, T., Ney, P. A., Wang, D., Rehg, J., Boyd, K., van Deursen, J. M. A., Brindle, P. K. (2006). Global transcriptional coactivators CREB-binding protein and p300 are highly essential collectively but not individually in peripheral B cells. Blood 107: 4407-4416 [Abstract] [Full Text]  
  • Kasper, L. H., Fukuyama, T., Biesen, M. A., Boussouar, F., Tong, C., de Pauw, A., Murray, P. J., van Deursen, J. M. A., Brindle, P. K. (2006). Conditional Knockout Mice Reveal Distinct Functions for the Global Transcriptional Coactivators CBP and p300 in T-Cell Development. Mol. Cell. Biol. 26: 789-809 [Abstract] [Full Text]  
  • Kennell, J. A., MacDougald, O. A. (2005). Wnt Signaling Inhibits Adipogenesis through {beta}-Catenin-dependent and -independent Mechanisms. J. Biol. Chem. 280: 24004-24010 [Abstract] [Full Text]  
  • Bennett, C. N., Longo, K. A., Wright, W. S., Suva, L. J., Lane, T. F., Hankenson, K. D., MacDougald, O. A. (2005). Regulation of osteoblastogenesis and bone mass by Wnt10b. Proc. Natl. Acad. Sci. USA 102: 3324-3329 [Abstract] [Full Text]  
  • Kang, S., Bajnok, L., Longo, K. A., Petersen, R. K., Hansen, J. B., Kristiansen, K., MacDougald, O. A. (2005). Effects of Wnt Signaling on Brown Adipocyte Differentiation and Metabolism Mediated by PGC-1{alpha}. Mol. Cell. Biol. 25: 1272-1282 [Abstract] [Full Text]  
  • Longo, K. A., Wright, W. S., Kang, S., Gerin, I., Chiang, S.-H., Lucas, P. C., Opp, M. R., MacDougald, O. A. (2004). Wnt10b Inhibits Development of White and Brown Adipose Tissues. J. Biol. Chem. 279: 35503-35509 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»