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Molecular and Cellular Biology, August 2003, p. 5606-5613, Vol. 23, No. 16
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.16.5606-5613.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Stem-Loop IV of Tetrahymena Telomerase RNA Stimulates Processivity in trans

Douglas X. Mason,{dagger} Elizabeth Goneska,{ddagger} and Carol W. Greider*

Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

Received 21 February 2003/ Returned for modification 18 April 2003/ Accepted 16 May 2003

Telomerase is a ribonucleoprotein enzyme responsible for the addition of telomeres onto the ends of chromosomes. Short or dysfunctional telomeres can lead to cell growth arrest, apoptosis, and genomic instability. Telomerase uses its RNA subunit to copy a short template region for telomere synthesis. To probe for regions of Tetrahymena telomerase RNA essential for function, we assayed 27 circularly permuted RNA deletions for telomerase in vitro activity and binding to the telomerase reverse transcriptase catalytic protein subunit. We found that stem-loop IV is required for wild-type telomerase activity in vitro and will stimulate processivity when added in trans.

* Corresponding author. Mailing address: Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 617 Hunterian Building, 725 N. Wolfe St., Baltimore, MD 21205. Phone: (410) 614-6506. Fax: (410) 614-2987. E-mail: cgreider{at}jhmi.edu.

{dagger} Present address: Roswell Park Cancer Institute, Buffalo, NY 14263.

{ddagger} Present address: Rockefeller University, New York, N.Y.

Molecular and Cellular Biology, August 2003, p. 5606-5613, Vol. 23, No. 16
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.16.5606-5613.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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