This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Landry, J.
Right arrow Articles by Sternglanz, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Landry, J.
Right arrow Articles by Sternglanz, R.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 2003, p. 5972-5978, Vol. 23, No. 17
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.17.5972-5978.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Set2-Catalyzed Methylation of Histone H3 Represses Basal Expression of GAL4 in Saccharomyces cerevisiae

Joseph Landry,1 Ann Sutton,2 Tina Hesman,3 Jinrong Min,4 Rui-Ming Xu,4 Mark Johnston,3 and Rolf Sternglanz2*

Program in Genetics,1 Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York 11794,2 Department of Genetics, Washington University Medical School, St. Louis, Missouri 63110,3 W. M. Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 117244

Received 2 May 2003/ Accepted 15 May 2003

Recent work has shown that histone methylation is an important regulator of transcription. While much is known about the roles of histone methyltransferases (HMTs) in the establishment of heterochromatin, little is known of their roles in the regulation of actively transcribed genes. We describe an in vivo role of the Saccharomyces cerevisiae HMT, Set2. We identified SET2 as a gene necessary for repression of GAL4 basal expression and show that the evolutionarily conserved SACI, SACII, and SET domains of Set2 are necessary for this repression. We confirm that Set2 catalyzes methylation of lysine 36 on the N-terminal tail of histone H3. Conversion of lysine 36 to an unmethylatable arginine causes a decrease in the repression of GAL4 transcription, as does a {Delta}set2 mutation. We further show that lysine 36 of histone H3 at GAL4 is methylated and that this methylation is dependent upon the presence of SET2.

* Corresponding author. Mailing address: Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794-5215. Phone: (631) 632-8565. Fax: (631) 632-8575. E-mail: rolf{at}life.bio.sunysb.edu.

Molecular and Cellular Biology, September 2003, p. 5972-5978, Vol. 23, No. 17
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.17.5972-5978.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Polytarchou, C., Pfau, R., Hatziapostolou, M., Tsichlis, P. N. (2008). The JmjC Domain Histone Demethylase Ndy1 Regulates Redox Homeostasis and Protects Cells from Oxidative Stress. Mol. Cell. Biol. 28: 7451-7464 [Abstract] [Full Text]  
  • Du, H.-N., Fingerman, I. M., Briggs, S. D. (2008). Histone H3 K36 methylation is mediated by a trans-histone methylation pathway involving an interaction between Set2 and histone H4. Genes Dev. 22: 2786-2798 [Abstract] [Full Text]  
  • Youdell, M. L., Kizer, K. O., Kisseleva-Romanova, E., Fuchs, S. M., Duro, E., Strahl, B. D., Mellor, J. (2008). Roles for Ctk1 and Spt6 in Regulating the Different Methylation States of Histone H3 Lysine 36. Mol. Cell. Biol. 28: 4915-4926 [Abstract] [Full Text]  
  • Smith, E. H., Janknecht, R., Maher, L. J. III (2007). Succinate inhibition of {alpha}-ketoglutarate-dependent enzymes in a yeast model of paraganglioma. Hum Mol Genet 16: 3136-3148 [Abstract] [Full Text]  
  • Andersen, E. C., Horvitz, H. R. (2007). Two C. elegans histone methyltransferases repress lin-3 EGF transcription to inhibit vulval development. Development 134: 2991-2999 [Abstract] [Full Text]  
  • Kim, A., Kiefer, C. M., Dean, A. (2007). Distinctive Signatures of Histone Methylation in Transcribed Coding and Noncoding Human {beta}-Globin Sequences. Mol. Cell. Biol. 27: 1271-1279 [Abstract] [Full Text]  
  • Strathdee, G., Sim, A., Soutar, R., Holyoake, T. L., Brown, R. (2007). HOXA5 is targeted by cell-type-specific CpG island methylation in normal cells and during the development of acute myeloid leukaemia. Carcinogenesis 28: 299-309 [Abstract] [Full Text]  
  • Vakoc, C. R., Sachdeva, M. M., Wang, H., Blobel, G. A. (2006). Profile of Histone Lysine Methylation across Transcribed Mammalian Chromatin. Mol. Cell. Biol. 26: 9185-9195 [Abstract] [Full Text]  
  • Workman, J. L. (2006). Nucleosome displacement in transcription. Genes Dev. 20: 2009-2017 [Abstract] [Full Text]  
  • Chu, Y., Sutton, A., Sternglanz, R., Prelich, G. (2006). The Bur1 Cyclin-Dependent Protein Kinase Is Required for the Normal Pattern of Histone Methylation by Set2. Mol. Cell. Biol. 26: 3029-3038 [Abstract] [Full Text]  
  • Rao, B., Shibata, Y., Strahl, B. D., Lieb, J. D. (2005). Dimethylation of Histone H3 at Lysine 36 Demarcates Regulatory and Nonregulatory Chromatin Genome-Wide. Mol. Cell. Biol. 25: 9447-9459 [Abstract] [Full Text]  
  • Adhvaryu, K. K., Morris, S. A., Strahl, B. D., Selker, E. U. (2005). Methylation of Histone H3 Lysine 36 Is Required for Normal Development in Neurospora crassa. Eukaryot Cell 4: 1455-1464 [Abstract] [Full Text]  
  • Bannister, A. J., Schneider, R., Myers, F. A., Thorne, A. W., Crane-Robinson, C., Kouzarides, T. (2005). Spatial Distribution of Di- and Tri-methyl Lysine 36 of Histone H3 at Active Genes. J. Biol. Chem. 280: 17732-17736 [Abstract] [Full Text]  
  • Kizer, K. O., Phatnani, H. P., Shibata, Y., Hall, H., Greenleaf, A. L., Strahl, B. D. (2005). A Novel Domain in Set2 Mediates RNA Polymerase II Interaction and Couples Histone H3 K36 Methylation with Transcript Elongation. Mol. Cell. Biol. 25: 3305-3316 [Abstract] [Full Text]  
  • Lee, D. Y., Teyssier, C., Strahl, B. D., Stallcup, M. R. (2005). Role of Protein Methylation in Regulation of Transcription. Endocr. Rev. 26: 147-170 [Abstract] [Full Text]  
  • Li, Y.-J., Stallcup, M. R., Lai, M. M. C. (2004). Hepatitis Delta Virus Antigen Is Methylated at Arginine Residues, and Methylation Regulates Subcellular Localization and RNA Replication. J. Virol. 78: 13325-13334 [Abstract] [Full Text]  
  • Carvin, C. D., Kladde, M. P. (2004). Effectors of Lysine 4 Methylation of Histone H3 in Saccharomyces cerevisiae Are Negative Regulators of PHO5 and GAL1-10. J. Biol. Chem. 279: 33057-33062 [Abstract] [Full Text]  
  • Cheng, H., He, X., Moore, C. (2004). The Essential WD Repeat Protein Swd2 Has Dual Functions in RNA Polymerase II Transcription Termination and Lysine 4 Methylation of Histone H3. Mol. Cell. Biol. 24: 2932-2943 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»