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Molecular and Cellular Biology, September 2003, p. 6267-6278, Vol. 23, No. 17
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.17.6267-6278.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The Critical cis-Acting Element Required for IMD2 Feedback Regulation by GDP Is a TATA Box Located 202 Nucleotides Upstream of the Transcription Start Site

Mafalda Escobar-Henriques,1,2,{dagger} Bertrand Daignan-Fornier,1 and Martine A. Collart2*

Institut de Biochimie et Génétique Cellulaires, CNRS UMR 5095, F-33077 Bordeaux Cedex, France,1 Département de Biochimie Médicale, CMU, CH-1211 Geneva 4, Switzerland2

Received 9 April 2003/ Accepted 3 June 2003

Guanylic nucleotides are essential cellular players, and the critical enzyme in their tightly regulated synthesis in Saccharomyces cerevisiae is encoded by the IMD2 gene. The transcription of IMD2 is subject to general repression by nutrient limitation through the cis nutrient-sensing element. It is also subject to specific feedback regulation by the end products of the guanylic nucleotide synthesis pathway. The critical cis element for this latter mechanism is the guanine response element (GRE), a TATAATA sequence which is located 202 nucleotides upstream of the transcription initiation site and which functions as the IMD2 TATA box. We show that the GRE functions in conjunction with a 52-nucleotide stretch near the transcription start site. This very unusual promoter structure ensures low, basal expression of IMD2 and the recruitment of TFIID to the GRE in response to guanylic nucleotide limitation.

* Corresponding author. Mailing address: Département de Biochimie Médicale, CMU, 1 Rue Michel Servet, CH-1211 Geneva 4, Switzerland. Phone: 41 22 702 54 76. Fax: 41 22 702 55 02. E-mail: martine.collart{at}medecine.unige.ch.

{dagger} Present address: Institute for Genetics, University of Cologne, D-50674 Cologne, Germany.

Molecular and Cellular Biology, September 2003, p. 6267-6278, Vol. 23, No. 17
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.17.6267-6278.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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