This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miyaji-Yamaguchi, M.
Right arrow Articles by Nagata, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miyaji-Yamaguchi, M.
Right arrow Articles by Nagata, K.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 2003, p. 6672-6684, Vol. 23, No. 18
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.18.6672-6684.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Involvement of Nucleocytoplasmic Shuttling of Yeast Nap1 in Mitotic Progression

Mary Miyaji-Yamaguchi,1,{dagger} Kohsuke Kato,1,2 Ryosuke Nakano,2,{ddagger} Tomohiro Akashi,3 Akihiko Kikuchi,3 and Kyosuke Nagata1*

Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba 305-8575,1 Department of Biological Information, Tokyo Institute of Technology, Yokohama 226-8501,2 Department of Medical Mycology, Nagoya University, Nagoya 466-8550, Japan3

Received 5 May 2003/ Accepted 24 June 2003

Nucleosome assembly protein 1 (Nap1) is widely conserved from yeasts to humans and facilitates nucleosome formation in vitro as a histone chaperone. Nap1 is generally localized in the cytoplasm, except that subcellular localization of Drosophila melanogaster Nap1 is dynamically regulated between the cytoplasm and nucleus during early development. The cytoplasmic localization of Nap1 is seemingly incompatible with the proposed role of Nap1 in nucleosome formation, which should occur in the nucleus. Here, we have examined the roles of a putative nuclear export signal (NES) sequence in yeast Nap1 (yNap1). yNap1 mutants lacking the NES-like sequence were localized predominantly in the nucleus. Deletion of NAP1 in cells harboring a single mitotic cyclin gene is known to cause mitotic delay and temperature-sensitive growth. A wild-type NAP1 complemented these phenotypes while nap1 mutant genes lacking the NES-like sequence or carboxy-terminal region did not. These and other results suggest that yNap1 is a nucleocytoplasmic shuttling protein and that its shuttling is important for yNap1 function during mitotic progression. This study also provides a possible explanation for Nap1's involvement in nucleosome assembly and/or remodeling in the nucleus.

* Corresponding author. Mailing address: Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan. Phone: 81-298-53-3233. Fax: 81-298-53-3134. E-mail: knagata{at}md.tsukuba.ac.jp.

{dagger} Present address: Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa 199-0195, Japan.

{ddagger} Present address: Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Tokyo 194-8533, Japan.

Molecular and Cellular Biology, September 2003, p. 6672-6684, Vol. 23, No. 18
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.18.6672-6684.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Calvert, M. E. K., Keck, K. M., Ptak, C., Shabanowitz, J., Hunt, D. F., Pemberton, L. F. (2008). Phosphorylation by Casein Kinase 2 Regulates Nap1 Localization and Function. Mol. Cell. Biol. 28: 1313-1325 [Abstract] [Full Text]  
  • Zlatanova, J., Seebart, C., Tomschik, M. (2007). Nap1: taking a closer look at a juggler protein of extraordinary skills. FASEB J. 21: 1294-1310 [Abstract] [Full Text]  
  • Naito, T., Momose, F., Kawaguchi, A., Nagata, K. (2007). Involvement of Hsp90 in Assembly and Nuclear Import of Influenza Virus RNA Polymerase Subunits. J. Virol. 81: 1339-1349 [Abstract] [Full Text]  
  • Galichet, A., Gruissem, W. (2006). Developmentally Controlled Farnesylation Modulates AtNAP1;1 Function in Cell Proliferation and Cell Expansion during Arabidopsis Leaf Development. Plant Physiol. 142: 1412-1426 [Abstract] [Full Text]  
  • Friedeberg, C., Scarlett, G., McGeeghan, J., Abu-daya, A., Guille, M., Kneale, G. (2006). Identification of a structural and functional domain in xNAP1 involved in protein-protein interactions. Nucleic Acids Res 34: 4893-4899 [Abstract] [Full Text]  
  • Navadgi, V. M., Chandra, B. R., Mishra, P. C., Sharma, A. (2006). The Two Plasmodium falciparum Nucleosome Assembly Proteins Play Distinct Roles in Histone Transport and Chromatin Assembly. J. Biol. Chem. 281: 16978-16984 [Abstract] [Full Text]  
  • Park, Y.-J., Luger, K. (2006). The structure of nucleosome assembly protein 1. Proc. Natl. Acad. Sci. USA 103: 1248-1253 [Abstract] [Full Text]  
  • Okuwaki, M., Kato, K., Shimahara, H., Tate, S.-i., Nagata, K. (2005). Assembly and Disassembly of Nucleosome Core Particles Containing Histone Variants by Human Nucleosome Assembly Protein I. Mol. Cell. Biol. 25: 10639-10651 [Abstract] [Full Text]  
  • Abu-Daya, A., Steer, W. M., Trollope, A. F., Friedeberg, C. E., Patient, R. K., Thorne, A. W., Guille, M. J. (2005). Zygotic nucleosome assembly protein-like 1 has a specific, non-cell autonomous role in hematopoiesis. Blood 106: 514-520 [Abstract] [Full Text]  
  • Dong, A., Liu, Z., Zhu, Y., Yu, F., Li, Z., Cao, K., Shen, W.-H. (2005). Interacting Proteins and Differences in Nuclear Transport Reveal Specific Functions for the NAP1 Family Proteins in Plants. Plant Physiol. 138: 1446-1456 [Abstract] [Full Text]  
  • Toth, K. F., Mazurkiewicz, J., Rippe, K. (2005). Association States of Nucleosome Assembly Protein 1 and Its Complexes with Histones. J. Biol. Chem. 280: 15690-15699 [Abstract] [Full Text]  
  • Kho, Y., Kim, S. C., Jiang, C., Barma, D., Kwon, S. W., Cheng, J., Jaunbergs, J., Weinbaum, C., Tamanoi, F., Falck, J., Zhao, Y. (2004). A tagging-via-substrate technology for detection and proteomics of farnesylated proteins. Proc. Natl. Acad. Sci. USA 101: 12479-12484 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»