This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Moreau, V.
Right arrow Articles by Génot, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Moreau, V.
Right arrow Articles by Génot, E.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, October 2003, p. 6809-6822, Vol. 23, No. 19
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.19.6809-6822.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Actin Can Reorganize into Podosomes in Aortic Endothelial Cells, a Process Controlled by Cdc42 and RhoA

Violaine Moreau,* Florence Tatin, Christine Varon, and Elisabeth Génot

Institut Européen de Chimie-Biologie, INSERM U441, 33600 Pessac, France

Received 15 January 2003/ Returned for modification 26 February 2003/ Accepted 24 June 2003

Members of the Rho GTPase family play a central role in the orchestration of cytoskeletal rearrangements, which are of prime importance in endothelial cell physiology. To explore their role in this specialized cell type, we used the bacterial toxin cytotoxic necrotizing factor 1 (CNF1) as a Rho GTPase activator. Punctate filamentous actin structures appeared along the ventral plasma membrane of endothelial cells and were identified as the core of podosomes by the distinctive vinculin ring around the F-actin. Rho, Rac, and Cdc42 were all identified as targets of CNF1, but only a constitutively active mutant of Cdc42 could substitute for CNF1 in podosome induction. Accordingly, organization of F-actin in these structures was highly dependent on the main Cdc42 cytoskeletal effector N-Wiskott-Aldrich syndrome protein. Other components of the actin machinery such as Arp2/3 and for the first time WIP also colocalized at these sites. Like CNF1 treatment, sustained Cdc42 activity induced a time-dependent F-actin-vinculin reorganization, prevented cytokinesis, and downregulated Rho activity. Finally, podosomes were also detected on endothelial cells explanted from patients undergoing cardiac surgery. These data provide the first description of podosomes in endothelial cells. The identification of such specialized structures opens up a new field of investigation in terms of endothelium pathophysiology.

* Corresponding author. Mailing address: INSERM U441, Institut Européen de Chimie-Biologie, Avenue du Haut-Lévêque, 33600 Pessac, France. Phone: 33 5 57890101. Fax: 33 5 56368979. E-mail: v.moreau{at}iecb-polytechnique.u-bordeaux.fr.

Molecular and Cellular Biology, October 2003, p. 6809-6822, Vol. 23, No. 19
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.19.6809-6822.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Dovas, A., Gevrey, J.-C., Grossi, A., Park, H., Abou-Kheir, W., Cox, D. (2009). Regulation of podosome dynamics by WASp phosphorylation: implication in matrix degradation and chemotaxis in macrophages. J. Cell Sci. 122: 3873-3882 [Abstract] [Full Text]  
  • Klein, I. K., Predescu, D. N., Sharma, T., Knezevic, I., Malik, A. B., Predescu, S. (2009). Intersectin-2L Regulates Caveola Endocytosis Secondary to Cdc42-mediated Actin Polymerization. J. Biol. Chem. 284: 25953-25961 [Abstract] [Full Text]  
  • Wang, J., Taba, Y., Pang, J., Yin, G., Yan, C., Berk, B. C. (2009). GIT1 Mediates VEGF-Induced Podosome Formation in Endothelial Cells: Critical Role for PLC{gamma}. Arterioscler. Thromb. Vasc. Bio. 29: 202-208 [Abstract] [Full Text]  
  • Abouzahr-Rifai, S., Hasmim, M., Boukerche, H., Hamelin, J., Janji, B., Jalil, A., Kieda, C., Mami-Chouaib, F., Bertoglio, J., Chouaib, S. (2008). Resistance of Tumor Cells to Cytolytic T Lymphocytes Involves Rho-GTPases and Focal Adhesion Kinase Activation. J. Biol. Chem. 283: 31665-31672 [Abstract] [Full Text]  
  • Guo, J., Ibaragi, S., Zhu, T., Luo, L.-Y., Hu, G.-F., Huppi, P. S., Chen, C. Y. (2008). Nicotine Promotes Mammary Tumor Migration via a Signaling Cascade Involving Protein Kinase C and cdc42. Cancer Res. 68: 8473-8481 [Abstract] [Full Text]  
  • Dorfleutner, A., Cho, Y., Vincent, D., Cunnick, J., Lin, H., Weed, S. A., Stehlik, C., Flynn, D. C. (2008). Phosphorylation of AFAP-110 affects podosome lifespan in A7r5 cells. J. Cell Sci. 121: 2394-2405 [Abstract] [Full Text]  
  • Guegan, F., Tatin, F., Leste-Lasserre, T., Drutel, G., Genot, E., Moreau, V. (2008). p190B RhoGAP regulates endothelial-cell-associated proteolysis through MT1-MMP and MMP2. J. Cell Sci. 121: 2054-2061 [Abstract] [Full Text]  
  • van Helden, S. F. G., Oud, M. M., Joosten, B., Peterse, N., Figdor, C. G., van Leeuwen, F. N. (2008). PGE2-mediated podosome loss in dendritic cells is dependent on actomyosin contraction downstream of the RhoA-Rho-kinase axis. J. Cell Sci. 121: 1096-1106 [Abstract] [Full Text]  
  • Badowski, C., Pawlak, G., Grichine, A., Chabadel, A., Oddou, C., Jurdic, P., Pfaff, M., Albiges-Rizo, C., Block, M. R. (2008). Paxillin Phosphorylation Controls Invadopodia/Podosomes Spatiotemporal Organization. Mol. Biol. Cell 19: 633-645 [Abstract] [Full Text]  
  • Chabadel, A., Banon-Rodriguez, I., Cluet, D., Rudkin, B. B., Wehrle-Haller, B., Genot, E., Jurdic, P., Anton, I. M., Saltel, F. (2007). CD44 and beta3 Integrin Organize Two Functionally Distinct Actin-based Domains in Osteoclasts. Mol. Biol. Cell 18: 4899-4910 [Abstract] [Full Text]  
  • Habib, A., Shamseddeen, I., Nasrallah, M. S., Antoun, T. A., Nemer, G., Bertoglio, J., Badreddine, R., Badr, K. F. (2007). Modulation of COX-2 expression by statins in human monocytic cells. FASEB J. 21: 1665-1674 [Abstract] [Full Text]  
  • Hoffmann, C., Aktories, K., Schmidt, G. (2007). Change in Substrate Specificity of Cytotoxic Necrotizing Factor Unmasks Proteasome-independent Down-regulation of Constitutively Active RhoA. J. Biol. Chem. 282: 10826-10832 [Abstract] [Full Text]  
  • Morita, T., Mayanagi, T., Yoshio, T., Sobue, K. (2007). Changes in the Balance between Caldesmon Regulated by p21-activated Kinases and the Arp2/3 Complex Govern Podosome Formation. J. Biol. Chem. 282: 8454-8463 [Abstract] [Full Text]  
  • Tsuboi, S. (2007). Requirement for a Complex of Wiskott-Aldrich Syndrome Protein (WASP) with WASP Interacting Protein in Podosome Formation in Macrophages. J. Immunol. 178: 2987-2995 [Abstract] [Full Text]  
  • Goicoechea, S., Arneman, D., Disanza, A., Garcia-Mata, R., Scita, G., Otey, C. A. (2006). Palladin binds to Eps8 and enhances the formation of dorsal ruffles and podosomes in vascular smooth muscle cells. J. Cell Sci. 119: 3316-3324 [Abstract] [Full Text]  
  • Wheeler, A. P., Wells, C. M., Smith, S. D., Vega, F. M., Henderson, R. B., Tybulewicz, V. L., Ridley, A. J. (2006). Rac1 and Rac2 regulate macrophage morphology but are not essential for migration. J. Cell Sci. 119: 2749-2757 [Abstract] [Full Text]  
  • Calle, Y., Carragher, N. O., Thrasher, A. J., Jones, G. E. (2006). Inhibition of calpain stabilises podosomes and impairs dendritic cell motility. J. Cell Sci. 119: 2375-2385 [Abstract] [Full Text]  
  • Varon, C., Tatin, F., Moreau, V., Van Obberghen-Schilling, E., Fernandez-Sauze, S., Reuzeau, E., Kramer, I., Genot, E. (2006). Transforming Growth Factor {beta} Induces Rosettes of Podosomes in Primary Aortic Endothelial Cells.. Mol. Cell. Biol. 26: 3582-3594 [Abstract] [Full Text]  
  • Collin, O., Tracqui, P., Stephanou, A., Usson, Y., Clement-Lacroix, J., Planus, E. (2006). Spatiotemporal dynamics of actin-rich adhesion microdomains: influence of substrate flexibility.. J. Cell Sci. 119: 1914-1925 [Abstract] [Full Text]  
  • Eves, R., Webb, B. A., Zhou, S., Mak, A. S. (2006). Caldesmon is an integral component of podosomes in smooth muscle cells. J. Cell Sci. 119: 1691-1702 [Abstract] [Full Text]  
  • Tatin, F., Varon, C., Genot, E., Moreau, V. (2006). A signalling cascade involving PKC, Src and Cdc42 regulates podosome assembly in cultured endothelial cells in response to phorbol ester. J. Cell Sci. 119: 769-781 [Abstract] [Full Text]  
  • Chaigne-Delalande, B., Deuve, L., Reuzeau, E., Basoni, C., Lafarge, D., Varon, C., Tatin, F., Anies, G., Garand, R., Kramer, I., Genot, E. (2006). RhoGTPases and p53 Are Involved in the Morphological Appearance and Interferon-{alpha} Response of Hairy Cells. Am. J. Pathol. 168: 562-573 [Abstract] [Full Text]  
  • Zhou, S., Webb, B. A., Eves, R., Mak, A. S. (2006). Effects of tyrosine phosphorylation of cortactin on podosome formation in A7r5 vascular smooth muscle cells. Am. J. Physiol. Cell Physiol. 290: C463-C471 [Abstract] [Full Text]  
  • Chellaiah, M. A. (2005). Regulation of Actin Ring Formation by Rho GTPases in Osteoclasts. J. Biol. Chem. 280: 32930-32943 [Abstract] [Full Text]  
  • Destaing, O., Saltel, F., Gilquin, B., Chabadel, A., Khochbin, S., Ory, S., Jurdic, P. (2005). A novel Rho-mDia2-HDAC6 pathway controls podosome patterning through microtubule acetylation in osteoclasts. J. Cell Sci. 118: 2901-2911 [Abstract] [Full Text]  
  • Burgstaller, G., Gimona, M. (2005). Podosome-mediated matrix resorption and cell motility in vascular smooth muscle cells. Am. J. Physiol. Heart Circ. Physiol. 288: H3001-H3005 [Abstract] [Full Text]  
  • Linder, S., Kopp, P. (2005). Podosomes at a glance. J. Cell Sci. 118: 2079-2082 [Full Text]  
  • Yamaguchi, H., Lorenz, M., Kempiak, S., Sarmiento, C., Coniglio, S., Symons, M., Segall, J., Eddy, R., Miki, H., Takenawa, T., Condeelis, J. (2005). Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp2/3 complex pathway and cofilin. JCB 168: 441-452 [Abstract] [Full Text]  
  • Berdeaux, R. L., Diaz, B., Kim, L., Martin, G. S. (2004). Active Rho is localized to podosomes induced by oncogenic Src and is required for their assembly and function. JCB 166: 317-323 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»