This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lim, F.-L.
Right arrow Articles by Sharrocks, A. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lim, F.-L.
Right arrow Articles by Sharrocks, A. D.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, January 2003, p. 450-461, Vol. 23, No. 2
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.2.450-461.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Mcm1p-Induced DNA Bending Regulates the Formation of Ternary Transcription Factor Complexes

Fei-Ling Lim,1,2,{dagger} Andrew Hayes,2 Adam G. West,1,{ddagger} Aline Pic-Taylor,1 Zoulfia Darieva,2 Brian A. Morgan,1 Stephen G. Oliver,2 and Andrew D. Sharrocks1,2*

Department of Biochemistry and Genetics, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH,1 School of Biological Sciences, University of Manchester, Manchester M13 9PT, United Kingdom2

Received 20 August 2002/ Returned for modification 30 September 2002/ Accepted 28 October 2002

The yeast MADS-box transcription factor Mcm1p plays an important regulatory role in several diverse cellular processes. In common with a subset of other MADS-box transcription factors, Mcm1p elicits substantial DNA bending. However, the role of protein-induced bending by MADS-box proteins in eukaryotic gene regulation is not understood. Here, we demonstrate an important role for Mcm1p-mediated DNA bending in determining local promoter architecture and permitting the formation of ternary transcription factor complexes. We constructed mutant mcm1 alleles that are defective in protein-induced bending. Defects in nuclear division, cell growth or viability, transcription, and gene expression were observed in these mutants. We identified one likely cause of the cell growth defects as the aberrant formation of the cell cycle-regulatory Fkh2p-Mcm1p complex. Microarray analysis confirmed the importance of Mcm1p-mediated DNA bending in maintaining correct gene expression profiles and revealed defects in Mcm1p-mediated repression of Ty elements and in the expression of the cell cycle-regulated YFR and CHS1 genes. Thus, we discovered an important role for DNA bending by MADS-box proteins in the formation and function of eukaryotic transcription factor complexes.

* Corresponding author. Mailing address: School of Biological Sciences, University of Manchester, 2.205 Stopford Building, Oxford Rd., Manchester M13 9PT, United Kingdom. Phone: 0044 161 275 5979. Fax: 0044 161 275 5082. E-mail: a.d.sharrocks{at}man.ac.uk.

{dagger} Present address: Syngenta, CTL, Alderley Park, Macclesfield, Cheshire SK10 4TJ, United Kingdom.

{ddagger} Present address: Laboratory of Molecular Biology, NIDDK, NIH, Bethesda, MD 20892-0540.

Molecular and Cellular Biology, January 2003, p. 450-461, Vol. 23, No. 2
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.2.450-461.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zaromytidou, A.-I., Miralles, F., Treisman, R. (2006). MAL and Ternary Complex Factor Use Different Mechanisms To Contact a Common Surface on the Serum Response Factor DNA-Binding Domain.. Mol. Cell. Biol. 26: 4134-4148 [Abstract] [Full Text]  
  • Oliver, S. G (2006). From genomes to systems: the path with yeast. Phil Trans R Soc B 361: 477-482 [Abstract] [Full Text]  
  • Sims, A. H., Gent, M. E., Lanthaler, K., Dunn-Coleman, N. S., Oliver, S. G., Robson, G. D. (2005). Transcriptome Analysis of Recombinant Protein Secretion by Aspergillus nidulans and the Unfolded-Protein Response In Vivo. Appl. Environ. Microbiol. 71: 2737-2747 [Abstract] [Full Text]  
  • Pérez-Lago, L., Salas, M., Camacho, A. (2005). A precise DNA bend angle is essential for the function of the phage {phi}29 transcriptional regulator. Nucleic Acids Res 33: 126-134 [Abstract] [Full Text]  
  • Carr, E. A., Mead, J., Vershon, A. K. (2004). {alpha}1-induced DNA bending is required for transcriptional activation by the Mcm1-{alpha}1 complex. Nucleic Acids Res 32: 2298-2305 [Abstract] [Full Text]  
  • Zhang, Y., Dufau, M. L. (2003). Repression of the Luteinizing Hormone Receptor Gene Promoter by Cross Talk among EAR3/COUP-TFI, Sp1/Sp3, and TFIIB. Mol. Cell. Biol. 23: 6958-6972 [Abstract] [Full Text]  
  • Boros, J., Lim, F.-L., Darieva, Z., Pic-Taylor, A., Harman, R., Morgan, B. A., Sharrocks, A. D. (2003). Molecular determinants of the cell-cycle regulated Mcm1p-Fkh2p transcription factor complex. Nucleic Acids Res 31: 2279-2288 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»