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Molecular and Cellular Biology, November 2003, p. 8352-8362, Vol. 23, No. 22
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.22.8352-8362.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The Papillomavirus E8{wedge}E2C Protein Represses DNA Replication from Extrachromosomal Origins

Thomas Zobel, Thomas Iftner, and Frank Stubenrauch*

Sektion Experimentelle Virologie, Institut für Medizinische Virologie und Epidemiologie der Viruskrankheiten, Universitätsklinikum Tübingen, D72076 Tübingen, Germany

Received 19 February 2003/ Returned for modification 2 April 2003/ Accepted 11 June 2003

Carcinogenic DNA viruses such as high-risk human papillomaviruses (HPV) and Epstein-Barr-Virus (EBV) replicate during persistent infections as low-copy-number plasmids. EBV DNA replication is restricted by host cell replication licensing mechanisms. In contrast, copy number control of HPV genomes is not under cellular control but involves the viral sequence-specific DNA-binding E2 activator and E8{wedge}E2C repressor proteins. Analysis of HPV31 mutant genomes revealed that residues outside of the DNA-binding/dimerization domain of E8{wedge}E2C limit viral DNA replication, indicating that binding site competition or heterodimerization among E2 and E8{wedge}E2C proteins does not contribute to copy number control. Domain swap experiments demonstrated that the amino-terminal 21 amino acids of E8{wedge}E2C represent a novel, transferable DNA replication repressor domain, whose activity requires conserved lysine and tryptophan residues. Furthermore, E8{wedge}E2C(1-21)-GAL4 fusion proteins inhibited the replication of the plasmid origin of replication of EBV, suggesting that E8{wedge}E2C functions as a general replication repressor of extrachromosomal origins. This finding could be important for the development of novel therapies against persistent DNA tumor virus infections.

* Corresponding author. Mailing address: Sektion Experimentelle Virologie, Institut für Medizinische Virologie und Epidemiologie der Viruskrankheiten, Universitätsklinikum Tübingen, Elfriede-Aulhorn-Str. 6, D72076 Tübingen, Germany. Phone: 49-7071-2981553. Fax: 49-7071-295419. E-mail: frank.stubenrauch{at}med.uni-tuebingen.de.

Molecular and Cellular Biology, November 2003, p. 8352-8362, Vol. 23, No. 22
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.22.8352-8362.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lace, M. J., Anson, J. R., Turek, L. P., Haugen, T. H. (2008). Functional Mapping of the Human Papillomavirus Type 16 E1 Cistron. J. Virol. 82: 10724-10734 [Abstract] [Full Text]  
  • Lace, M. J., Anson, J. R., Thomas, G. S., Turek, L. P., Haugen, T. H. (2008). The E8{wedge}E2 Gene Product of Human Papillomavirus Type 16 Represses Early Transcription and Replication but Is Dispensable for Viral Plasmid Persistence in Keratinocytes. J. Virol. 82: 10841-10853 [Abstract] [Full Text]  
  • Ammermann, I., Bruckner, M., Matthes, F., Iftner, T., Stubenrauch, F. (2008). Inhibition of Transcription and DNA Replication by the Papillomavirus E8^E2C Protein Is Mediated by Interaction with Corepressor Molecules. J. Virol. 82: 5127-5136 [Abstract] [Full Text]  


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