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Molecular and Cellular Biology, December 2003, p. 9275-9282, Vol. 23, No. 24
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.24.9275-9282.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Affinity Purification of Specific Chromatin Segments from Chromosomal Loci in Yeast

Joachim Griesenbeck, Hinrich Boeger, J. Seth Strattan, and Roger D. Kornberg*

Department of Structural Biology, Stanford University School of Medicine, Stanford, California 94305

Received 13 June 2003/ Returned for modification 18 August 2003/ Accepted 10 September 2003

Single-copy gene and promoter regions have been excised from yeast chromosomes and have been purified as chromatin by conventional and affinity methods. Promoter regions isolated in transcriptionally repressed and activated states maintain their characteristic chromatin structures. Gel filtration analysis establishes the uniformity of the transcriptionally activated state. Activator proteins interact in the manner anticipated from previous studies in vivo. This work opens the way to the direct study of specific gene regions of eukaryotic chromosomes in diverse functional and structural states.

* Corresponding author. Mailing address: Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305. Phone: (650) 723-6988. Fax: (650) 723-8464. E-mail: kornberg{at}stanford.edu.

Molecular and Cellular Biology, December 2003, p. 9275-9282, Vol. 23, No. 24
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.24.9275-9282.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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