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Molecular and Cellular Biology, February 2003, p. 988-1003, Vol. 23, No. 3
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.3.988-1003.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Transcriptional Activities of the Zinc Finger Protein Zac Are Differentially Controlled by DNA Binding

Anke Hoffmann,1 Elisabetta Ciani,1 Joel Boeckardt,2 Florian Holsboer,1 Laurent Journot,2 and Dietmar Spengler1*

Molecular Neuroendocrinology, Max Planck Institute of Psychiatry, D-80804 Munich, Germany,1 UPR 9023 Centre National de la Recherche Scientifique, F-34094 Montpellier Cedex 5, France2

Received 6 May 2002/ Returned for modification 18 June 2002/ Accepted 30 October 2002

Zac encodes a zinc finger protein that promotes apoptosis and cell cycle arrest and is maternally imprinted. Here, we show that Zac contains transactivation and repressor activities and that these transcriptional activities are differentially controlled by DNA binding. Zac transactivation mapped to two distinct domains. One of these contained multiple repeats of the peptide PLE, which behaved as an autonomous activation unit. More importantly, we identified two related high-affinity DNA-binding sites which were differentially bound by seven Zac C2H2 zinc fingers. Zac bound as a monomer through zinc fingers 6 and 7 to the palindromic DNA element to confer transactivation. In contrast, binding as a monomer to one half-site of the repeat element turned Zac into a repressor. Conversely, Zac dimerization at properly spaced direct and reverse repeat elements enabled transactivation, which strictly correlated with DNA-dependent and -independent contacts of key residues within the recognition helix of zinc finger 7. The later ones support specific functional connections between Zac DNA binding and transcriptional-regulatory surfaces. Both classes of DNA elements were identified in a new Zac target gene and confirmed that the zinc fingers communicate with the transactivation function. Together, our data demonstrate a role for Zac as a transcription factor in addition to its role as coactivator for nuclear receptors and p53.

* Corresponding author. Mailing address: Molecular Neuroendocrinology, Max Planck Institute of Psychiatry, Kraepelinstrasse 2-10, D-80804 Munich, Germany. Phone: 49 89 30622 559. Fax: 49 89 30622 605. E-mail: spengler{at}mpipsykl.mpg.de.

Molecular and Cellular Biology, February 2003, p. 988-1003, Vol. 23, No. 3
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.3.988-1003.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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