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Molecular and Cellular Biology, February 2003, p. 1341-1348, Vol. 23, No. 4
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.4.1341-1348.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Snf1 Kinases with Different ß-Subunit Isoforms Play Distinct Roles in Regulating Haploid Invasive Growth

Valmik K. Vyas,1 Sergei Kuchin,2 Cristin D. Berkey,2 and Marian Carlson2,3*

Integrated Program in Cellular, Molecular and Biophysical Studies,1 Departments of Genetics and Development,2 Microbiology, Columbia University, New York, New York 100323

Received 4 October 2002/ Returned for modification 13 November 2002/ Accepted 21 November 2002

The Snf1 protein kinase of Saccharomyces cerevisiae has been shown to have a role in regulating haploid invasive growth in response to glucose depletion. Cells contain three forms of the Snf1 kinase, each with a different ß-subunit isoform, either Gal83, Sip1, or Sip2. We present evidence that different Snf1 kinases play distinct roles in two aspects of invasive growth, namely, adherence to the agar substrate and filamentation. The Snf1-Gal83 form of the kinase is required for adherence, whereas either Snf1-Gal83 or Snf1-Sip2 is sufficient for filamentation. Genetic evidence indicates that Snf1-Gal83 affects adherence by antagonizing Nrg1- and Nrg2-mediated repression of the FLO11 flocculin and adhesin gene. In contrast, the mechanism(s) by which Snf1-Gal83 and Snf1-Sip2 affect filamentation is independent of FLO11. Thus, the Snf1 kinase regulates invasive growth by at least two distinct mechanisms.

* Corresponding author. Mailing address: 701 W. 168th St., HSC922, New York, NY 10032. Phone: (212) 305-6314. Fax: (212) 305-1741. E-mail: mbc1{at}columbia.edu.

Molecular and Cellular Biology, February 2003, p. 1341-1348, Vol. 23, No. 4
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.4.1341-1348.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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