This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Aylon, Y.
Right arrow Articles by Kupiec, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aylon, Y.
Right arrow Articles by Kupiec, M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, February 2003, p. 1403-1417, Vol. 23, No. 4
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.4.1403-1417.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular Dissection of Mitotic Recombination in the Yeast Saccharomyces cerevisiae

Yael Aylon, Batia Liefshitz, Gili Bitan-Banin, and Martin Kupiec*

Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv 69978, Israel

Received 28 August 2002/ Returned for modification 10 October 2002/ Accepted 18 November 2002

Recombination plays a central role in the repair of broken chromosomes in all eukaryotes. We carried out a systematic study of mitotic recombination. Using several assays, we established the chronological sequence of events necessary to repair a single double-strand break. Once a chromosome is broken, yeast cells become immediately committed to recombinational repair. Recombination is completed within an hour and exhibits two kinetic gaps. By using this kinetic framework we also characterized the role played by several proteins in the recombinational process. In the absence of Rad52, the broken chromosome ends, both 5' and 3', are rapidly degraded. This is not due to the inability to recombine, since the 3' single-stranded DNA ends are stable in a strain lacking donor sequences. Rad57 is required for two consecutive strand exchange reactions. Surprisingly, we found that the Srs2 helicase also plays an early positive role in the recombination process.

* Corresponding author. Mailing address: Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv 69978, Israel. Phone: 972-3-640-9031. Fax: 972-3-640-9407. E-mail: martin{at}post.tau.ac.il.

Molecular and Cellular Biology, February 2003, p. 1403-1417, Vol. 23, No. 4
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.4.1403-1417.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Colavito, S., Macris-Kiss, M., Seong, C., Gleeson, O., Greene, E. C., Klein, H. L., Krejci, L., Sung, P. (2009). Functional significance of the Rad51-Srs2 complex in Rad51 presynaptic filament disruption. Nucleic Acids Res 37: 6754-6764 [Abstract] [Full Text]  
  • Blanck, S., Kobbe, D., Hartung, F., Fengler, K., Focke, M., Puchta, H. (2009). A SRS2 homolog from Arabidopsis thaliana disrupts recombinogenic DNA intermediates and facilitates single strand annealing. Nucleic Acids Res 0: gkp753v1-gkp753 [Abstract] [Full Text]  
  • Agmon, N., Pur, S., Liefshitz, B., Kupiec, M. (2009). Analysis of repair mechanism choice during homologous recombination. Nucleic Acids Res 37: 5081-5092 [Abstract] [Full Text]  
  • Fung, C. W., Mozlin, A. M., Symington, L. S. (2009). Suppression of the Double-Strand-Break-Repair Defect of the Saccharomyces cerevisiae rad57 Mutant. Genetics 181: 1195-1206 [Abstract] [Full Text]  
  • Rothenberg, E., Grimme, J. M., Spies, M., Ha, T. (2008). Human Rad52-mediated homology search and annealing occurs by continuous interactions between overlapping nucleoprotein complexes. Proc. Natl. Acad. Sci. USA 105: 20274-20279 [Abstract] [Full Text]  
  • Lyndaker, A. M., Goldfarb, T., Alani, E. (2008). Mutants Defective in Rad1-Rad10-Slx4 Exhibit a Unique Pattern of Viability During Mating-Type Switching in Saccharomyces cerevisiae. Genetics 179: 1807-1821 [Abstract] [Full Text]  
  • Welz-Voegele, C., Jinks-Robertson, S. (2008). Sequence Divergence Impedes Crossover More Than Noncrossover Events During Mitotic Gap Repair in Yeast. Genetics 179: 1251-1262 [Abstract] [Full Text]  
  • Wu, Y., Kantake, N., Sugiyama, T., Kowalczykowski, S. C. (2008). Rad51 Protein Controls Rad52-mediated DNA Annealing. J. Biol. Chem. 283: 14883-14892 [Abstract] [Full Text]  
  • Mozlin, A. M., Fung, C. W., Symington, L. S. (2008). Role of the Saccharomyces cerevisiae Rad51 Paralogs in Sister Chromatid Recombination. Genetics 178: 113-126 [Abstract] [Full Text]  
  • Branzei, D., Foiani, M. (2007). RecQ helicases queuing with Srs2 to disrupt Rad51 filaments and suppress recombination. Genes Dev. 21: 3019-3026 [Full Text]  
  • Chiolo, I., Saponaro, M., Baryshnikova, A., Kim, J.-H., Seo, Y.-S., Liberi, G. (2007). The Human F-Box DNA Helicase FBH1 Faces Saccharomyces cerevisiae Srs2 and Postreplication Repair Pathway Roles. Mol. Cell. Biol. 27: 7439-7450 [Abstract] [Full Text]  
  • Kent, N. A., Chambers, A. L., Downs, J. A. (2007). Dual Chromatin Remodeling Roles for RSC during DNA Double Strand Break Induction and Repair at the Yeast MAT Locus. J. Biol. Chem. 282: 27693-27701 [Abstract] [Full Text]  
  • Wu, Y., Siino, J. S., Sugiyama, T., Kowalczykowski, S. C. (2006). The DNA Binding Preference of RAD52 and RAD59 Proteins: IMPLICATIONS FOR RAD52 AND RAD59 PROTEIN FUNCTION IN HOMOLOGOUS RECOMBINATION. J. Biol. Chem. 281: 40001-40009 [Abstract] [Full Text]  
  • Symington, L. S., Heyer, W.-D. (2006). Some disassembly required: role of DNA translocases in the disruption of recombination intermediates and dead-end complexes. Genes Dev. 20: 2479-2486 [Full Text]  
  • Cheng, C.-H., Lo, Y.-H., Liang, S.-S., Ti, S.-C., Lin, F.-M., Yeh, C.-H., Huang, H.-Y., Wang, T.-F. (2006). SUMO modifications control assembly of synaptonemal complex and polycomplex in meiosis of Saccharomyces cerevisiae. Genes Dev. 20: 2067-2081 [Abstract] [Full Text]  
  • Liberi, G., Maffioletti, G., Lucca, C., Chiolo, I., Baryshnikova, A., Cotta-Ramusino, C., Lopes, M., Pellicioli, A., Haber, J. E., Foiani, M. (2005). Rad51-dependent DNA structures accumulate at damaged replication forks in sgs1 mutants defective in the yeast ortholog of BLM RecQ helicase. Genes Dev. 19: 339-350 [Abstract] [Full Text]  
  • Llorente, B., Symington, L. S. (2004). The Mre11 Nuclease Is Not Required for 5' to 3' Resection at Multiple HO-Induced Double-Strand Breaks. Mol. Cell. Biol. 24: 9682-9694 [Abstract] [Full Text]  
  • Krejci, L., Macris, M., Li, Y., Van Komen, S., Villemain, J., Ellenberger, T., Klein, H., Sung, P. (2004). Role of ATP Hydrolysis in the Antirecombinase Function of Saccharomyces cerevisiae Srs2 Protein. J. Biol. Chem. 279: 23193-23199 [Abstract] [Full Text]  
  • Jazayeri, A., McAinsh, A. D., Jackson, S. P. (2004). Saccharomyces cerevisiae Sin3p facilitates DNA double-strand break repair. Proc. Natl. Acad. Sci. USA 101: 1644-1649 [Abstract] [Full Text]  
  • Storici, F., Durham, C. L., Gordenin, D. A., Resnick, M. A. (2003). Chromosomal site-specific double-strand breaks are efficiently targeted for repair by oligonucleotides in yeast. Proc. Natl. Acad. Sci. USA 100: 14994-14999 [Abstract] [Full Text]  
  • Van Komen, S., Reddy, M. S., Krejci, L., Klein, H., Sung, P. (2003). ATPase and DNA Helicase Activities of the Saccharomyces cerevisiae Anti-recombinase Srs2. J. Biol. Chem. 278: 44331-44337 [Abstract] [Full Text]  
  • Sung, P., Krejci, L., Van Komen, S., Sehorn, M. G. (2003). Rad51 Recombinase and Recombination Mediators. J. Biol. Chem. 278: 42729-42732 [Full Text]  
  • Aylon, Y., Kupiec, M. (2003). The Checkpoint Protein Rad24 of Saccharomyces cerevisiae Is Involved in Processing Double-Strand Break Ends and in Recombination Partner Choice. Mol. Cell. Biol. 23: 6585-6596 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»