This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vincent, O.
Right arrow Articles by Peñalva, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vincent, O.
Right arrow Articles by Peñalva, M. A.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, March 2003, p. 1647-1655, Vol. 23, No. 5
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.5.1647-1655.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

YPXL/I Is a Protein Interaction Motif Recognized by Aspergillus PalA and Its Human Homologue, AIP1/Alix

Olivier Vincent,1* Lynne Rainbow,2,{dagger} Joan Tilburn,2 Herbert N. Arst, Jr.,2 and Miguel A. Peñalva1

Departamento de Microbiología Molecular, Centro de Investigaciones Biológicas del CSIC, Madrid 28006, Spain,1 Department of Infectious Diseases, Faculty of Medicine, Imperial College of Science, Technology & Medicine, London W12 0NN, United Kingdom2

Received 4 October 2002/ Returned for modification 21 November 2002/ Accepted 2 December 2002

The zinc finger transcription factor PacC undergoes two-step proteolytic activation in response to alkaline ambient pH. PalA is a component of the fungal ambient pH signal transduction pathway. Its mammalian homologue AIP1/Alix interacts with the apoptosis-linked protein ALG-2. We show that both PalA and AIP1/Alix recognize a protein-protein binding motif that we denote YPXL/I, where Tyr, Pro, and Leu/Ile are crucial for its interactive properties. Two such motifs flanking the signaling protease cleavage site mediate direct binding of PalA to PacC, required for the first and only pH-regulated cleavage of this transcription factor. PalA can bind the "closed" (i.e., wild-type full-length) conformer of PacC, suggesting that PalA binding constitutes the first stage in the two-step proteolytic cascade, recruiting or facilitating access of the signaling protease, presumably PalB. In addition to recognizing YPXL/I motifs, both PalA and AIP1/Alix interact with the Aspergillus class E Vps protein Vps32 homologue, a member of a protein complex involved in the early steps of the multivesicular body pathway, suggesting that this interaction is an additional feature of proteins of the PalA/AIP1/Alix family.

* Corresponding author. Mailing address: Departamento de Microbiologia Molecular, Centro de Investigaciones Biológicas del CSIC, Velázquez 144, Madrid 28006, Spain. Phone: 34 91 5644562 ext. 4357. Fax: 34 91 5627518. E-mail: ovincent{at}cib.csic.es.

{dagger} Present address: Division of Reproductive and Child Health, Medical and Molecular Genetics, The Medical School, Edgbaston, Birmingham B15 2TT, United Kingdom.

Molecular and Cellular Biology, March 2003, p. 1647-1655, Vol. 23, No. 5
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.5.1647-1655.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Rodriguez-Galan, O., Galindo, A., Hervas-Aguilar, A., Arst, H. N. Jr., Penalva, M. A. (2009). Physiological Involvement in pH Signaling of Vps24-mediated Recruitment of Aspergillus PalB Cysteine Protease to ESCRT-III. J. Biol. Chem. 284: 4404-4412 [Abstract] [Full Text]  
  • McCullough, J., Fisher, R. D., Whitby, F. G., Sundquist, W. I., Hill, C. P. (2008). ALIX-CHMP4 interactions in the human ESCRT pathway. Proc. Natl. Acad. Sci. USA 105: 7687-7691 [Abstract] [Full Text]  
  • Blanchin-Roland, S., Da Costa, G., Gaillardin, C. (2008). Ambient pH signalling in the yeast Yarrowia lipolytica involves YlRim23p/PalC, which interacts with Snf7p/Vps32p, but does not require the long C terminus of YlRim9p/PalI. Microbiology 154: 1668-1676 [Abstract] [Full Text]  
  • Yorikawa, C., Takaya, E., Osako, Y., Tanaka, R., Terasawa, Y., Hamakubo, T., Mochizuki, Y., Iwanari, H., Kodama, T., Maeda, T., Hitomi, K., Shibata, H., Maki, M. (2008). Human Calpain 7/PalBH Associates with a Subset of ESCRT-III-related Proteins in its N-terminal Region and Partly Localizes to Endocytic Membrane Compartments. J Biochem 143: 731-745 [Abstract] [Full Text]  
  • Calcagno-Pizarelli, A. M., Negrete-Urtasun, S., Denison, S. H., Rudnicka, J. D., Bussink, H.-J., Munera-Huertas, T., Stanton, L., Hervas-Aguilar, A., Espeso, E. A., Tilburn, J., Arst, H. N. Jr., Penalva, M. A. (2007). Establishment of the Ambient pH Signaling Complex in Aspergillus nidulans: PalI Assists Plasma Membrane Localization of PalH. Eukaryot Cell 6: 2365-2375 [Abstract] [Full Text]  
  • Nikko, E., Andre, B. (2007). Split-Ubiquitin Two-Hybrid Assay To Analyze Protein-Protein Interactions at the Endosome: Application to Saccharomyces cerevisiae Bro1 Interacting with ESCRT Complexes, the Doa4 Ubiquitin Hydrolase, and the Rsp5 Ubiquitin Ligase. Eukaryot Cell 6: 1266-1277 [Abstract] [Full Text]  
  • Penas, M. M., Hervas-Aguilar, A., Munera-Huertas, T., Reoyo, E., Penalva, M. A., Arst, H. N. Jr., Tilburn, J. (2007). Further Characterization of the Signaling Proteolysis Step in the Aspergillus nidulans pH Signal Transduction Pathway. Eukaryot Cell 6: 960-970 [Abstract] [Full Text]  
  • Munshi, U. M., Kim, J., Nagashima, K., Hurley, J. H., Freed, E. O. (2007). An Alix Fragment Potently Inhibits HIV-1 Budding: CHARACTERIZATION OF BINDING TO RETROVIRAL YPXL LATE DOMAINS. J. Biol. Chem. 282: 3847-3855 [Abstract] [Full Text]  
  • Langelier, C., von Schwedler, U. K., Fisher, R. D., De Domenico, I., White, P. L., Hill, C. P., Kaplan, J., Ward, D., Sundquist, W. I. (2006). Human ESCRT-II Complex and Its Role in Human Immunodeficiency Virus Type 1 Release. J. Virol. 80: 9465-9480 [Abstract] [Full Text]  
  • Odorizzi, G. (2006). The multiple personalities of Alix.. J. Cell Sci. 119: 3025-3032 [Abstract] [Full Text]  
  • Boysen, J. H., Mitchell, A. P. (2006). Control of Bro1-Domain Protein Rim20 Localization by External pH, ESCRT Machinery, and the Saccharomyces cerevisiae Rim101 Pathway. Mol. Biol. Cell 17: 1344-1353 [Abstract] [Full Text]  
  • Chen, C., Vincent, O., Jin, J., Weisz, O. A., Montelaro, R. C. (2005). Functions of Early (AP-2) and Late (AIP1/ALIX) Endocytic Proteins in Equine Infectious Anemia Virus Budding. J. Biol. Chem. 280: 40474-40480 [Abstract] [Full Text]  
  • Blanchin-Roland, S., Costa, G. D., Gaillardin, C. (2005). ESCRT-I components of the endocytic machinery are required for Rim101-dependent ambient pH regulation in the yeast Yarrowia lipolytica. Microbiology 151: 3627-3637 [Abstract] [Full Text]  
  • Tilburn, J., Sanchez-Ferrero, J. C., Reoyo, E., Arst, H. N. Jr., Penalva, M. A. (2005). Mutational Analysis of the pH Signal Transduction Component PalC of Aspergillus nidulans Supports Distant Similarity to BRO1 Domain Family Members. Genetics 171: 393-401 [Abstract] [Full Text]  
  • Herranz, S., Rodriguez, J. M., Bussink, H.-J., Sanchez-Ferrero, J. C., Arst, H. N. Jr., Penalva, M. A., Vincent, O. (2005). Arrestin-related proteins mediate pH signaling in fungi. Proc. Natl. Acad. Sci. USA 102: 12141-12146 [Abstract] [Full Text]  
  • Ohneda, M., Arioka, M., Kitamoto, K. (2005). Isolation and Characterization of Aspergillus oryzae Vacuolar Protein Sorting Mutants. Appl. Environ. Microbiol. 71: 4856-4861 [Abstract] [Full Text]  
  • Patton, G. S., Morris, S. A., Chung, W., Bieniasz, P. D., McClure, M. O. (2005). Identification of Domains in Gag Important for Prototypic Foamy Virus Egress. J. Virol. 79: 6392-6399 [Abstract] [Full Text]  
  • Schmitt, A. P., Leser, G. P., Morita, E., Sundquist, W. I., Lamb, R. A. (2005). Evidence for a New Viral Late-Domain Core Sequence, FPIV, Necessary for Budding of a Paramyxovirus. J. Virol. 79: 2988-2997 [Abstract] [Full Text]  
  • Kullas, A. L., Li, M., Davis, D. A. (2004). Snf7p, a Component of the ESCRT-III Protein Complex, Is an Upstream Member of the RIM101 Pathway in Candida albicans. Eukaryot Cell 3: 1609-1618 [Abstract] [Full Text]  
  • Xu, W., Smith, F. J. Jr., Subaran, R., Mitchell, A. P. (2004). Multivesicular Body-ESCRT Components Function in pH Response Regulation in Saccharomyces cerevisiae and Candida albicans. Mol. Biol. Cell 15: 5528-5537 [Abstract] [Full Text]  
  • Bache, K. G., Slagsvold, T., Cabezas, A., Rosendal, K. R., Raiborg, C., Stenmark, H. (2004). The Growth-Regulatory Protein HCRP1/hVps37A Is a Subunit of Mammalian ESCRT-I and Mediates Receptor Down-Regulation. Mol. Biol. Cell 15: 4337-4346 [Abstract] [Full Text]  
  • Martin-Serrano, J., Perez-Caballero, D., Bieniasz, P. D. (2004). Context-Dependent Effects of L Domains and Ubiquitination on Viral Budding. J. Virol. 78: 5554-5563 [Abstract] [Full Text]  
  • Shehu-Xhilaga, M., Ablan, S., Demirov, D. G., Chen, C., Montelaro, R. C., Freed, E. O. (2004). Late Domain-Dependent Inhibition of Equine Infectious Anemia Virus Budding. J. Virol. 78: 724-732 [Abstract] [Full Text]  
  • Nikko, E., Marini, A.-M., Andre, B. (2003). Permease Recycling and Ubiquitination Status Reveal a Particular Role for Bro1 in the Multivesicular Body Pathway. J. Biol. Chem. 278: 50732-50743 [Abstract] [Full Text]  
  • Martin-Serrano, J., Yarovoy, A., Perez-Caballero, D., Bieniasz, P. D. (2003). Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins. Proc. Natl. Acad. Sci. USA 100: 12414-12419 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»