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Molecular and Cellular Biology, March 2003, p. 1798-1807, Vol. 23, No. 5
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.5.1798-1807.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Nob1p Is Required for Cleavage of the 3' End of 18S rRNA

Alessandro Fatica,1 Marlene Oeffinger,1 Mensur Dlakic,2 and David Tollervey1*

Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom,1 Department of Biological Chemistry, The University of Michigan Medical School, Ann Arbor, Michigan 48109-06062

Received 7 October 2002/ Returned for modification 26 November 2002/ Accepted 2 December 2002

We report the characterization of a novel factor, Nob1p (Yor056c), which is essential for the synthesis of 40S ribosome subunits. Genetic depletion of Nob1p strongly inhibits the processing of the 20S pre-rRNA to the mature 18S rRNA, leading to the accumulation of high levels of the 20S pre-rRNA together with novel degradation intermediates. 20S processing occurs within a pre-40S particle after its export from the nucleus to the cytoplasm. Consistent with a direct role in this cleavage, Nob1p was shown to be associated with the pre-40S particle and to be present in both the nucleus and the cytoplasm. This suggests that Nob1p accompanies the pre-40S ribosomes during nuclear export. Pre-40S export is not, however, inhibited by depletion of Nob1p.

* Corresponding author. Mailing address: Wellcome Trust Centre for Cell Biology, Swann Building, King's Buildings, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom. Phone: 44 131 650 7092. Fax: 44 131 650 7040. E-mail: d.tollervey{at}ed.ac.uk.

Molecular and Cellular Biology, March 2003, p. 1798-1807, Vol. 23, No. 5
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.5.1798-1807.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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