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Molecular and Cellular Biology, March 2003, p. 2083-2095, Vol. 23, No. 6
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.6.2083-2095.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Late Cytoplasmic Maturation of the Small Ribosomal Subunit Requires RIO Proteins in Saccharomyces cerevisiae

Emmanuel Vanrobays, Jean-Paul Gelugne,* Pierre-Emmanuel Gleizes, and Michele Caizergues-Ferrer

Laboratoire de Biologie Moléculaire Eucaryote du CNRS, 31062 Toulouse Cedex, France

Received 1 August 2002/ Returned for modification 13 September 2002/ Accepted 19 December 2002

Numerous nonribosomal trans-acting factors involved in pre-rRNA processing have been characterized, but few of them are specifically required for the last cytoplasmic steps of 18S rRNA maturation. We have recently demonstrated that Rrp10p/Rio1p is such a factor. By BLAST analysis, we identified the product of a previously uncharacterized essential gene, YNL207W/RIO2, called Rio2p, that shares 43% sequence similarity with Rrp10p/Rio1p. Rio2p homologues were identified throughout the Archaea and metazoan species. We show that Rio2p is a cytoplasmic-nuclear protein and that its depletion blocks 18S rRNA production, leading to 20S pre-rRNA accumulation. In situ hybridization reveals that in Rio2p-depleted cells, 20S pre-rRNA localizes in the cytoplasm, demonstrating that its accumulation is not due to an export defect. We also show that both Rio1p and Rio2p accumulate in the nucleus of crm1-1 cells at the nonpermissive temperature. Nuclear as well as cytoplasmic Rio2p and Rio1p cosediment with pre-40S particles. These results strongly suggest that Rio2p and Rrp10p/Rio1p are shuttling proteins which associate with pre-40S particles in the nucleus and they are not necessary for export of the pre-40S complexes but are absolutely required for the cytoplasmic maturation of 20S pre-rRNA at site D, leading to mature 40S ribosomal subunits.

* Corresponding author. Mailing address: LBME du CNRS, 118 route de Narbonne, 31062 Toulouse cedex, France. Phone: 33 5 61 33 59 58. Fax: 33 5 61 33 58 86. E-mail: jpg{at}ibcg.biotoul.fr.

Molecular and Cellular Biology, March 2003, p. 2083-2095, Vol. 23, No. 6
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.6.2083-2095.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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