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Molecular and Cellular Biology, April 2003, p. 2907-2913, Vol. 23, No. 8
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.8.2907-2913.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Annexin A5 Is Not Essential for Skeletal Development

Bent Brachvogel,1 Jörg Dikschas,1 Helga Moch,1 Heike Welzel,1,2,{dagger} Klaus von der Mark,1 Clementine Hofmann,1,2,{dagger} and Ernst Pöschl1*

Universität Erlangen-Nürnberg, Nikolaus-Fiebiger-Zentrum, Experimentelle Medizin I, Erlangen,1 GSF-Forschungszentrum für Umwelt und Gesundheit, Institut für Säugetiergenetik, Neuherberg, Oberschleissheim-Munich, Germany2

Received 16 September 2002/ Returned for modification 23 October 2002/ Accepted 10 December 2002

Annexins are highly conserved proteins that are characterized by their ability to interact with phospholipids in a calcium-dependent manner. Although diverse functions have been ascribed to annexins based on in vitro analyses, their in vivo functions still remain unclear. The intensively studied annexin A5 has been identified by its effects on blood coagulation, and subsequently, its function as a calcium-specific ion channel was described. In vitro experiments and expression studies suggested a potential role of annexin A5 during calcification processes in vivo, especially in endochondral ossification. To gain insights into the relevance of annexin A5 in this process, we generated an annexin A5-deficient mouse mutant. Mice lacking annexin A5 are viable, are fertile, and reveal no significant alterations in the biochemical parameters characteristic for metabolic or functional defects. Neither the development of skeletal elements nor the in vitro calcification properties of isolated chondrocytes is significantly impaired by the absence of annexin A5. Therefore, annexin A5 is dispensable for the formation and maintenance of skeletal elements in the mouse and may possibly be pointing to a compensatory effect of other members from the annexin family due to their high functional and structural similarity.

* Corresponding author. Mailing address: Friedrich-Alexander Universität Erlangen-Nürnberg, Nikolaus-Fiebiger-Zentrum, Lehrstuhl für Experimentelle Medizin I, Glückstr. 6, 91054 Erlangen, Germany. Phone: 49 9131 85 29102. Fax: 49 9131 85 26341. E-mail: epoeschl{at}molmed.uni-erlangen.de.

{dagger} Present address: Max-Planck-Institut für Psychiatrie, Munich, Germany.

Molecular and Cellular Biology, April 2003, p. 2907-2913, Vol. 23, No. 8
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.8.2907-2913.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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