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Molecular and Cellular Biology, April 2003, p. 3008-3012, Vol. 23, No. 8
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.8.3008-3012.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Deoxynucleotide Triphosphate Binding Mode Conserved in Y Family DNA Polymerases

Robert E. Johnson,1 José Trincao,2 Aneel K. Aggarwal,2 Satya Prakash,1 and Louise Prakash1*

Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston, Galveston, Texas 77555-1061,1 Structural Biology Program, Department of Physiology and Biophysics, Mt. Sinai School of Medicine, New York, New York 100292

Received 10 December 2002/ Returned for modification 20 January 2003/ Accepted 27 January 2003

Although DNA polymerase {eta} (Pol{eta}) and other Y family polymerases differ in sequence and function from classical DNA polymerases, they all share a similar right-handed architecture with the palm, fingers, and thumb domains. Here, we examine the role in Saccharomyces cerevisiae Pol{eta} of three conserved residues, tyrosine 64, arginine 67, and lysine 279, which come into close contact with the triphosphate moiety of the incoming nucleotide, in nucleotide incorporation. We find that mutational alteration of these residues reduces the efficiency of correct nucleotide incorporation very considerably. The high degree of conservation of these residues among the various Y family DNA polymerases suggests that these residues are also crucial for nucleotide incorporation in the other members of the family. Furthermore, we note that tyrosine 64 and arginine 67 are functionally equivalent to the deoxynucleotide triphosphate binding residues arginine 518 and histidine 506 in T7 DNA polymerase, respectively.

* Corresponding author. Mailing address: Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston, 6.104 Blocker Medical Research Building, 11th and Mechanic St., Galveston, TX 77555-1061. Phone: (409) 747-8601. Fax: (409) 747-8608. E-mail: l.prakash{at}utmb.edu.

Molecular and Cellular Biology, April 2003, p. 3008-3012, Vol. 23, No. 8
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.8.3008-3012.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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