This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Moratz, C.
Right arrow Articles by Kehrl, J. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Moratz, C.
Right arrow Articles by Kehrl, J. H.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, July 2004, p. 5767-5775, Vol. 24, No. 13
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.13.5767-5775.2004

Abnormal B-Cell Responses to Chemokines, Disturbed Plasma Cell Localization, and Distorted Immune Tissue Architecture in Rgs1–/– Mice

Chantal Moratz,1,{dagger} J. Russell Hayman,3,{dagger} Hua Gu,2 and John H. Kehrl1*

Laboratories of Immunoregulation,1 Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892,2 Department of Microbiology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee 376143

Received 22 December 2003/ Returned for modification 9 January 2004/ Accepted 24 March 2004

Normal lymphoid tissue development and function depend upon chemokine-directed cell migration. Since chemokines signal through heterotrimeric G-protein-coupled receptors, RGS proteins, which act as GTPase-activating proteins for G{alpha} subunits, likely fine tune the cellular responses to chemokines. Here we show that Rgs1–/– mice possess B cells that respond excessively and desensitize improperly to the chemokines CXCL12 and CXCL13. Many of the B-cell follicles in the spleens of Rgs1–/– mice have germinal centers even in the absence of immune stimulation. Furthermore, immunization of these mice leads to exaggerated germinal center formation; partial disruption of the normal architecture of the spleen and Peyer's patches; and abnormal trafficking of immunoglobulin-secreting cells. These results reveal the importance of a regulatory mechanism that limits and desensitizes chemokine receptor signaling.

* Corresponding author. Mailing address: Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bldg. 10, Room 11B08, 10 Center Dr., MSC 1876, Bethesda, MD 20892. Phone: (301) 402-4852. Fax: (301) 402-0070. E-mail: jkehrl{at}niaid.nih.gov.

{dagger} C.M. and J.R.H. contributed equally to this work.

Molecular and Cellular Biology, July 2004, p. 5767-5775, Vol. 24, No. 13
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.13.5767-5775.2004




This article has been cited by other articles:

  • Bansal, G., DiVietro, J. A., Kuehn, H. S., Rao, S., Nocka, K. H., Gilfillan, A. M., Druey, K. M. (2008). RGS13 Controls G Protein-Coupled Receptor-Evoked Responses of Human Mast Cells. J. Immunol. 181: 7882-7890 [Abstract] [Full Text]  
  • Tomayko, M. M., Anderson, S. M., Brayton, C. E., Sadanand, S., Steinel, N. C., Behrens, T. W., Shlomchik, M. J. (2008). Systematic Comparison of Gene Expression between Murine Memory and Naive B Cells Demonstrates That Memory B Cells Have Unique Signaling Capabilities. J. Immunol. 181: 27-38 [Abstract] [Full Text]  
  • Feuerer, M., Jiang, W., Holler, P. D., Satpathy, A., Campbell, C., Bogue, M., Mathis, D., Benoist, C. (2007). Enhanced thymic selection of FoxP3+ regulatory T cells in the NOD mouse model of autoimmune diabetes. Proc. Natl. Acad. Sci. USA 104: 18181-18186 [Abstract] [Full Text]  
  • Piovan, E., Tosello, V., Indraccolo, S., Masiero, M., Persano, L., Esposito, G., Zamarchi, R., Ponzoni, M., Chieco-Bianchi, L., Dalla-Favera, R., Amadori, A. (2007). Differential Regulation of Hypoxia-Induced CXCR4 Triggering during B-Cell Development and Lymphomagenesis. Cancer Res. 67: 8605-8614 [Abstract] [Full Text]  
  • Lattin, J., Zidar, D. A., Schroder, K., Kellie, S., Hume, D. A., Sweet, M. J. (2007). G-protein-coupled receptor expression, function, and signaling in macrophages. J. Leukoc. Biol. 82: 16-32 [Abstract] [Full Text]  
  • Hwang, I.-Y., Park, C., Kehrl, J. H. (2007). Impaired Trafficking of Gnai2+/- and Gnai2-/- T Lymphocytes: Implications for T Cell Movement within Lymph Nodes. J. Immunol. 179: 439-448 [Abstract] [Full Text]  
  • Han, J.-I., Huang, N.-N., Kim, D.-U., Kehrl, J. H. (2006). RGS1 and RGS13 mRNA silencing in a human B lymphoma line enhances responsiveness to chemoattractants and impairs desensitization. J. Leukoc. Biol. 79: 1357-1368 [Abstract] [Full Text]  
  • Huang, N.-N., Han, S.-B., Hwang, I.-Y., Kehrl, J. H. (2005). B Cells Productively Engage Soluble Antigen-Pulsed Dendritic Cells: Visualization of Live-Cell Dynamics of B Cell-Dendritic Cell Interactions. J. Immunol. 175: 7125-7134 [Abstract] [Full Text]  
  • Berthebaud, M., Riviere, C., Jarrier, P., Foudi, A., Zhang, Y., Compagno, D., Galy, A., Vainchenker, W., Louache, F. (2005). RGS16 is a negative regulator of SDF-1-CXCR4 signaling in megakaryocytes. Blood 106: 2962-2968 [Abstract] [Full Text]  
  • Le, Y., Honczarenko, M., Glodek, A. M., Ho, D. K., Silberstein, L. E. (2005). CXC Chemokine Ligand 12-Induced Focal Adhesion Kinase Activation and Segregation into Membrane Domains Is Modulated by Regulator of G Protein Signaling 1 in Pro-B Cells. J. Immunol. 174: 2582-2590 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»