This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Aho, S.
Right arrow Articles by Klement, J. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aho, S.
Right arrow Articles by Klement, J. F.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, July 2004, p. 6410-6418, Vol. 24, No. 14
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.14.6410-6418.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Periplakin Gene Targeting Reveals a Constituent of the Cornified Cell Envelope Dispensable for Normal Mouse Development

Sirpa Aho,1,{dagger}* Kehua Li,1,{dagger} Young Ryoo,1,{dagger} Clair McGee,1 Akemi Ishida-Yamamoto,2 Jouni Uitto,1 and John F. Klement1

Department of Dermatology and Cutaneous Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania,1 Department of Dermatology, Asahikawa Medical College, Asahikawa 078-8510, Japan2

Received 5 February 2004/ Accepted 9 April 2004

The members of the plakin family of proteins serve as epidermal cytolinkers and components of cell-cell and cell-matrix adhesion complexes, i.e., desmosomes and hemidesmosomes, respectively. Periplakin is a recently characterized member of this family. Human and mouse periplakin genomic loci are conserved, and the proteins are highly homologous, suggesting a role for periplakin in vertebrate physiology. In order to evaluate the functional role of periplakin, we generated periplakin null mice through targeted homologous recombination of mouse embryonic stem cells, followed by development of Ppl–/– mice. Mice homozygous for the targeted allele were born in the expected Mendelian frequency, developed normally, possessed grossly normal epidermis and hair, and were healthy and fertile. The epidermal barrier appeared to develop normally during fetal days E15.5 to E16.5, and the cornified envelope and desmosomes in the newborn mice were ultrastructurally normal. No compensatory increase in the expression of other epithelial proteins was detected in the neonatal mouse epidermis lacking periplakin. Consequently, the primary role of periplakin may not relate to the physiology of the cornified cell envelope in epidermal keratinocytes but may reside in the challenges, which normal laboratory mice do not encounter.

* Corresponding author. Mailing address: Thomas Jefferson University, Department of Dermatology and Cutaneous Biology, BLSB 422, 233 South Tenth St., Philadelphia, PA 19107. Phone: (215) 503-2018. Fax: (215) 503-5788. E-mail: Sirpa.Aho{at}jefferson.edu.

{dagger} S.A., K.L., and Y.R. contributed equally to this work.

Molecular and Cellular Biology, July 2004, p. 6410-6418, Vol. 24, No. 14
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.14.6410-6418.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sevilla, L. M., Nachat, R., Groot, K. R., Klement, J. F., Uitto, J., Djian, P., Maatta, A., Watt, F. M. (2007). Mice deficient in involucrin, envoplakin, and periplakin have a defective epidermal barrier. JCB 179: 1599-1612 [Abstract] [Full Text]  
  • Spazierer, D., Fuchs, P., Reipert, S., Fischer, I., Schmuth, M., Lassmann, H., Wiche, G. (2006). Epiplakin Is Dispensable for Skin Barrier Function and for Integrity of Keratin Network Cytoarchitecture in Simple and Stratified Epithelia. Mol. Cell. Biol. 26: 559-568 [Abstract] [Full Text]  
  • Jang, S.-I., Kalinin, A., Takahashi, K., Marekov, L. N., Steinert, P. M. (2005). Characterization of human epiplakin: RNAi-mediated epiplakin depletion leads to the disruption of keratin and vimentin IF networks. J. Cell Sci. 118: 781-793 [Abstract] [Full Text]  
  • Groot, K. R., Sevilla, L. M., Nishi, K., DiColandrea, T., Watt, F. M. (2004). Kazrin, a novel periplakin-interacting protein associated with desmosomes and the keratinocyte plasma membrane. JCB 166: 653-659 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»