This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grimson, A.
Right arrow Articles by Anderson, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grimson, A.
Right arrow Articles by Anderson, P.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 2004, p. 7483-7490, Vol. 24, No. 17
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.17.7483-7490.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SMG-1 Is a Phosphatidylinositol Kinase-Related Protein Kinase Required for Nonsense-Mediated mRNA Decay in Caenorhabditis elegans

Andrew Grimson,{dagger} Sean O'Connor,{dagger},{ddagger} Carrie Loushin Newman, and Philip Anderson*

Department of Genetics, University of Wisconsin, Madison, Wisconsin

Received 1 March 2004/ Returned for modification 9 April 2004/ Accepted 1 June 2004

Eukaryotic messenger RNAs containing premature stop codons are selectively and rapidly degraded, a phenomenon termed nonsense-mediated mRNA decay (NMD). Previous studies with both Caenohabditis elegans and mammalian cells indicate that SMG-2/human UPF1, a central regulator of NMD, is phosphorylated in an SMG-1-dependent manner. We report here that smg-1, which is required for NMD in C. elegans, encodes a protein kinase of the phosphatidylinositol kinase superfamily of protein kinases. We identify null alleles of smg-1 and demonstrate that SMG-1 kinase activity is required in vivo for NMD and in vitro for SMG-2 phosphorylation. SMG-1 and SMG-2 coimmunoprecipitate from crude extracts, and this interaction is maintained in smg-3 and smg-4 mutants, both of which are required for SMG-2 phosphorylation in vivo and in vitro. SMG-2 is located diffusely through the cytoplasm, and its location is unaltered in mutants that disrupt the cycle of SMG-2 phosphorylation. We discuss the role of SMG-2 phosphorylation in NMD.

* Corresponding author. Mailing address: Department of Genetics, University of Wisconsin, 445 Henry Mall, Madison, WI 53706. Phone: (608) 263-8429. Fax: (608) 262-2976. E-mail: andersn{at}wisc.edu.

{dagger} A.G. and S.O. contributed equally to this work.

{ddagger} Present address: Department of Blood and Marrow Transplantation, University of Texas, Houston, TX 77030.

Molecular and Cellular Biology, September 2004, p. 7483-7490, Vol. 24, No. 17
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.17.7483-7490.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Dang, Y., Low, W.-K., Xu, J., Gehring, N. H., Dietz, H. C., Romo, D., Liu, J. O. (2009). Inhibition of Nonsense-mediated mRNA Decay by the Natural Product Pateamine A through Eukaryotic Initiation Factor 4AIII. J. Biol. Chem. 284: 23613-23621 [Abstract] [Full Text]  
  • Yamashita, A., Izumi, N., Kashima, I., Ohnishi, T., Saari, B., Katsuhata, Y., Muramatsu, R., Morita, T., Iwamatsu, A., Hachiya, T., Kurata, R., Hirano, H., Anderson, P., Ohno, S. (2009). SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay. Genes Dev. 23: 1091-1105 [Abstract] [Full Text]  
  • Atkins, J. F., Bjork, G. R. (2009). A Gripping Tale of Ribosomal Frameshifting: Extragenic Suppressors of Frameshift Mutations Spotlight P-Site Realignment. Microbiol. Mol. Biol. Rev. 73: 178-210 [Abstract] [Full Text]  
  • Rabbitts, B. M., Ciotti, M. K., Miller, N. E., Kramer, M., Lawrenson, A. L., Levitte, S., Kremer, S., Kwan, E., Weis, A. M., Hermann, G. J. (2008). glo-3, a Novel Caenorhabditis elegans Gene, Is Required for Lysosome-Related Organelle Biogenesis. Genetics 180: 857-871 [Abstract] [Full Text]  
  • Dinkelmann, M. V., Zhang, H., Skop, A. R., White, J. G. (2007). SPD-3 Is Required for Spindle Alignment in Caenorhabditis elegans Embryos and Localizes to Mitochondria. Genetics 177: 1609-1620 [Abstract] [Full Text]  
  • Chang, Y.-F., Chan, W.-K., Imam, J. S., Wilkinson, M. F. (2007). Alternatively Spliced T-cell Receptor Transcripts Are Up-regulated in Response to Disruption of Either Splicing Elements or Reading Frame. J. Biol. Chem. 282: 29738-29747 [Abstract] [Full Text]  
  • Updike, D. L., Mango, S. E. (2007). Genetic Suppressors of Caenorhabditis elegans pha-4/FoxA Identify the Predicted AAA Helicase ruvb-1/RuvB. Genetics 177: 819-833 [Abstract] [Full Text]  
  • Culbertson, M. R. (2007). Navigating Without a Road Map. Genetics 177: 1-7 [Full Text]  
  • Johns, L., Grimson, A., Kuchma, S. L., Newman, C. L., Anderson, P. (2007). Caenorhabditis elegans SMG-2 Selectively Marks mRNAs Containing Premature Translation Termination Codons. Mol. Cell. Biol. 27: 5630-5638 [Abstract] [Full Text]  
  • Isken, O., Maquat, L. E. (2007). Quality control of eukaryotic mRNA: safeguarding cells from abnormal mRNA function. Genes Dev. 21: 1833-3856 [Abstract] [Full Text]  
  • Longman, D., Plasterk, R. H.A., Johnstone, I. L., Caceres, J. F. (2007). Mechanistic insights and identification of two novel factors in the C. elegans NMD pathway. Genes Dev. 21: 1075-1085 [Abstract] [Full Text]  
  • Morita, T., Yamashita, A., Kashima, I., Ogata, K., Ishiura, S., Ohno, S. (2007). Distant N- and C-terminal Domains Are Required for Intrinsic Kinase Activity of SMG-1, a Critical Component of Nonsense-mediated mRNA Decay. J. Biol. Chem. 282: 7799-7808 [Abstract] [Full Text]  
  • Phillips, B. T., Kidd, A. R. III, King, R., Hardin, J., Kimble, J. (2007). Reciprocal asymmetry of SYS-1/beta-catenin and POP-1/TCF controls asymmetric divisions in Caenorhabditis elegans. Proc. Natl. Acad. Sci. USA 104: 3231-3236 [Abstract] [Full Text]  
  • Katic, I., Greenwald, I. (2006). EMB-4: A Predicted ATPase That Facilitates lin-12 Activity in Caenorhabditis elegans. Genetics 174: 1907-1915 [Abstract] [Full Text]  
  • Sundaram, P., Echalier, B., Han, W., Hull, D., Timmons, L. (2006). ATP-binding Cassette Transporters Are Required for Efficient RNA Interference in Caenorhabditis elegans. Mol. Biol. Cell 17: 3678-3688 [Abstract] [Full Text]  
  • Wang, W., Cajigas, I. J., Peltz, S. W., Wilkinson, M. F., Gonzalez, C. I. (2006). Role for Upf2p Phosphorylation in Saccharomyces cerevisiae Nonsense-Mediated mRNA Decay.. Mol. Cell. Biol. 26: 3390-3400 [Abstract] [Full Text]  
  • Wittmann, J., Hol, E. M., Jack, H.-M. (2006). hUPF2 Silencing Identifies Physiologic Substrates of Mammalian Nonsense-Mediated mRNA Decay. Mol. Cell. Biol. 26: 1272-1287 [Abstract] [Full Text]  
  • Ford, A. S., Guan, Q., Neeno-Eckwall, E., Culbertson, M. R. (2006). Ebs1p, a Negative Regulator of Gene Expression Controlled by the Upf Proteins in the Yeast Saccharomyces cerevisiae. Eukaryot Cell 5: 301-312 [Abstract] [Full Text]  
  • Kashima, I., Yamashita, A., Izumi, N., Kataoka, N., Morishita, R., Hoshino, S., Ohno, M., Dreyfuss, G., Ohno, S. (2006). Binding of a novel SMG-1-Upf1-eRF1-eRF3 complex (SURF) to the exon junction complex triggers Upf1 phosphorylation and nonsense-mediated mRNA decay. Genes Dev. 20: 355-367 [Abstract] [Full Text]  
  • Maquat, L. E. (2005). Nonsense-mediated mRNA decay in mammals. J. Cell Sci. 118: 1773-1776 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»