This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fujioka, S.
Right arrow Articles by Chiao, P. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fujioka, S.
Right arrow Articles by Chiao, P. J.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 2004, p. 7806-7819, Vol. 24, No. 17
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.17.7806-7819.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

NF-{kappa}B and AP-1 Connection: Mechanism of NF-{kappa}B-Dependent Regulation of AP-1 Activity

Shuichi Fujioka,1,2,{dagger} Jiangong Niu,1,3,{dagger} Christian Schmidt,1,2 Guido M. Sclabas,1,4 Bailu Peng,1,2 Tadashi Uwagawa,1 Zhongkui Li,1 Douglas B. Evans,1 James L. Abbruzzese,5 and Paul J. Chiao1,2,3*

Department of Surgical Oncology,1 Department of Molecular and Cellular Oncology,2 Department of Gastrointestinal Medical Oncology, The University of Texas M. D. Anderson Cancer Center,5 The Program in Cancer Biology, The University of Texas Graduate School of Biomedical Sciences at Houston, Houston, Texas,3 Department of Visceral and Transplantation Surgery, Inselspital, University of Bern, Bern, Switzerland4

Received 8 March 2004/ Returned for modification 5 May 2004/ Accepted 17 May 2004

Nuclear factor {kappa}B (NF-{kappa}B) and activator protein 1 (AP-1) transcription factors regulate many important biological and pathological processes. Activation of NF-{kappa}B is regulated by the inducible phosphorylation of NF-{kappa}B inhibitor I{kappa}B by I{kappa}B kinase. In contrast, Fos, a key component of AP-1, is primarily transcriptionally regulated by serum responsive factors (SRFs) and ternary complex factors (TCFs). Despite these different regulatory mechanisms, there is an intriguing possibility that NF-{kappa}B and AP-1 may modulate each other, thus expanding the scope of these two rapidly inducible transcription factors. To determine whether NF-{kappa}B activity is involved in the regulation of fos expression in response to various stimuli, we analyzed activity of AP-1 and expression of fos, fosB, fra-1, fra-2, jun, junB, and junD, as well as AP-1 downstream target gene VEGF, using MDAPanc-28 and MDAPanc-28/I{kappa}B{alpha}M pancreatic tumor cells and wild-type, IKK1–/–, and IKK2–/– murine embryonic fibroblast cells. Our results show that elk-1, a member of TCFs, is one of the NF-{kappa}B downstream target genes. Inhibition of NF-{kappa}B activity greatly decreased expression of elk-1. Consequently, the reduced level of activated Elk-1 protein by extracellular signal-regulated kinase impeded constitutive, serum-, and superoxide-inducible c-fos expression. Thus, our study revealed a distinct and essential role of NF-{kappa}B in participating in the regulation of elk-1, c-fos, and VEGF expression.

* Corresponding author. Mailing address: Department of Molecular & Cellular Oncology and Department of Surgical Oncology, Box 107, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030. Phone: (713) 794-1030. Fax: (713) 794-4830. E-mail: pjchiao{at}mdanderson.org.

{dagger} S.F. and J.N. contributed equally to this study.

Molecular and Cellular Biology, September 2004, p. 7806-7819, Vol. 24, No. 17
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.17.7806-7819.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ndlovu, M. N., Van Lint, C., Van Wesemael, K., Callebert, P., Chalbos, D., Haegeman, G., Vanden Berghe, W. (2009). Hyperactivated NF-{kappa}B and AP-1 Transcription Factors Promote Highly Accessible Chromatin and Constitutive Transcription across the Interleukin-6 Gene Promoter in Metastatic Breast Cancer Cells. Mol. Cell. Biol. 29: 5488-5504 [Abstract] [Full Text]  
  • Luna, M. S. A., Hortencio, T. M. A., Ferreira, Z. S., Yamanouye, N. (2009). Sympathetic outflow activates the venom gland of the snake Bothrops jararaca by regulating the activation of transcription factors and the synthesis of venom gland proteins. J. Exp. Biol. 212: 1535-1543 [Abstract] [Full Text]  
  • Shin, S., Asano, T., Yao, Y., Zhang, R., Claret, F.-X., Korc, M., Sabapathy, K., Menter, D. G., Abbruzzese, J. L., Reddy, S. A.G. (2009). Activator Protein-1 Has an Essential Role in Pancreatic Cancer Cells and Is Regulated by a Novel Akt-Mediated Mechanism. Mol Cancer Res 7: 745-754 [Abstract] [Full Text]  
  • Kang, M.-I., Henrich, C. J., Bokesch, H. R., Gustafson, K. R., McMahon, J. B., Baker, A. R., Young, M. R., Colburn, N. H. (2009). A selective small-molecule nuclear factor-{kappa}B inhibitor from a high-throughput cell-based assay for "activator protein-1 hits". Molecular Cancer Therapeutics 8: 571-581 [Abstract] [Full Text]  
  • Kasperczyk, H., Baumann, B., Debatin, K.-M., Fulda, S. (2009). Characterization of sonic hedgehog as a novel NF-{kappa}B target gene that promotes NF-{kappa}B-mediated apoptosis resistance and tumor growth in vivo. FASEB J. 23: 21-33 [Abstract] [Full Text]  
  • Cahill, C. M., Rogers, J. T. (2008). Interleukin (IL) 1{beta} Induction of IL-6 Is Mediated by a Novel Phosphatidylinositol 3-Kinase-dependent AKT/I{kappa}B Kinase {alpha} Pathway Targeting Activator Protein-1. J. Biol. Chem. 283: 25900-25912 [Abstract] [Full Text]  
  • Shyu, Y. J., Suarez, C. D., Hu, C.-D. (2008). Visualization of AP-1 NF-{kappa}B ternary complexes in living cells by using a BiFC-based FRET. Proc. Natl. Acad. Sci. USA 105: 151-156 [Abstract] [Full Text]  
  • Chapoval, S. P., Al-Garawi, A., Lora, J. M., Strickland, I., Ma, B., Lee, P. J., Homer, R. J., Ghosh, S., Coyle, A. J., Elias, J. A. (2007). Inhibition of NF-{kappa}B Activation Reduces the Tissue Effects of Transgenic IL-13. J. Immunol. 179: 7030-7041 [Abstract] [Full Text]  
  • Muddasani, P., Norman, J. C., Ellman, M., van Wijnen, A. J., Im, H.-J. (2007). Basic Fibroblast Growth Factor Activates the MAPK and NF{kappa}B Pathways That Converge on Elk-1 to Control Production of Matrix Metalloproteinase-13 by Human Adult Articular Chondrocytes. J. Biol. Chem. 282: 31409-31421 [Abstract] [Full Text]  
  • Mizukami, Y., Kohgo, Y., Chung, D. C. (2007). Hypoxia Inducible Factor-1 Independent Pathways in Tumor Angiogenesis. Clin. Cancer Res. 13: 5670-5674 [Abstract] [Full Text]  
  • Arab, S., Konstantinov, I. E., Boscarino, C., Cukerman, E., Mori, A., Li, J., Liu, P. P., Redington, A. N., Coles, J. G. (2007). Early gene expression profiles during intraoperative myocardial ischemia-reperfusion in cardiac surgery. J. Thorac. Cardiovasc. Surg. 134: 74-81 [Abstract] [Full Text]  
  • Yamashita, T., Yao, Z., Li, F., Zhang, Q., Badell, I. R., Schwarz, E. M., Takeshita, S., Wagner, E. F., Noda, M., Matsuo, K., Xing, L., Boyce, B. F. (2007). NF-{kappa}B p50 and p52 Regulate Receptor Activator of NF-{kappa}B Ligand (RANKL) and Tumor Necrosis Factor-induced Osteoclast Precursor Differentiation by Activating c-Fos and NFATc1. J. Biol. Chem. 282: 18245-18253 [Abstract] [Full Text]  
  • Fleetwood, A. J., Lawrence, T., Hamilton, J. A., Cook, A. D. (2007). Granulocyte-Macrophage Colony-Stimulating Factor (CSF) and Macrophage CSF-Dependent Macrophage Phenotypes Display Differences in Cytokine Profiles and Transcription Factor Activities: Implications for CSF Blockade in Inflammation. J. Immunol. 178: 5245-5252 [Abstract] [Full Text]  
  • Remoli, M. E., Ragimbeau, J., Giacomini, E., Gafa, V., Severa, M., Lande, R., Pellegrini, S., Coccia, E. M. (2007). NF-{kappa}B is required for STAT-4 expression during dendritic cell maturation. J. Leukoc. Biol. 81: 355-363 [Abstract] [Full Text]  
  • Wang, X., Wang, Q., Ives, K. L., Evers, B. M. (2006). Curcumin Inhibits Neurotensin-Mediated Interleukin-8 Production and Migration of HCT116 Human Colon Cancer Cells.. Clin. Cancer Res. 12: 5346-5355 [Abstract] [Full Text]  
  • Kwon, S. J., Lee, Y. J. (2005). Effect of Low Glutamine/Glucose on Hypoxia-Induced Elevation of Hypoxia-Inducible Factor-1{alpha} in Human Pancreatic Cancer MiaPaCa-2 and Human Prostatic Cancer DU-145 Cells. Clin. Cancer Res. 11: 4694-4700 [Abstract] [Full Text]  
  • Teoh, M. L. T., Turner, P. V., Evans, D. H. (2005). Tumorigenic Poxviruses Up-Regulate Intracellular Superoxide To Inhibit Apoptosis and Promote Cell Proliferation. J. Virol. 79: 5799-5811 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»