This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rastegar, M.
Right arrow Articles by Featherstone, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rastegar, M.
Right arrow Articles by Featherstone, M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 2004, p. 8090-8103, Vol. 24, No. 18
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.18.8090-8103.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Sequential Histone Modifications at Hoxd4 Regulatory Regions Distinguish Anterior from Posterior Embryonic Compartments

Mojgan Rastegar,1,{dagger} Laila Kobrossy,1,2,{dagger} Erzsebet Nagy Kovacs,1 Isabel Rambaldi,1 and Mark Featherstone1,2,3*

McGill Cancer Centre,1 Department of Biochemistry,2 Department of Oncology, McGill University, Montreal, Quebec, Canada3

Received 16 October 2003/ Returned for modification 22 December 2003/ Accepted 14 June 2004

Hox genes are differentially expressed along the embryonic anteroposterior axis. We used chromatin immunoprecipitation to detect chromatin changes at the Hoxd4 locus during neurogenesis in P19 cells and embryonic day 8.0 (E8.0) and E10.5 mouse embryos. During Hoxd4 induction in both systems, we observed that histone modifications typical of transcriptionally active chromatin occurred first at the 3' neural enhancer and then at the promoter. Moreover, the sequential distribution of histone modifications between E8.0 and E10.5 was consistent with a spreading of open chromatin, starting with the enhancer, followed by successively more 5' intervening sequences, and culminating at the promoter. Neither RNA polymerase II (Pol II) nor CBP associated with the inactive gene. During Hoxd4 induction, CBP and RNA Pol II were recruited first to the enhancer and then to the promoter. Whereas the CBP association was transient, RNA Pol II remained associated with both regulatory regions. Histone modification and transcription factor recruitment occurred in posterior, Hox-expressing embryonic tissues, but never in anterior tissues, where such genes are inactive. Together, our observations demonstrate that the direction of histone modifications at Hoxd4 mirrors colinear gene activation across Hox clusters and that the establishment of anterior and posterior compartments is accompanied by the imposition of distinct chromatin states.

* Corresponding author. Mailing address: McGill Cancer Centre, McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec, Canada H3G 1Y6. Phone: (514) 398-8937. Fax: (514) 398-6769. E-mail: mark.featherstone{at}mcgill.ca.

{dagger} M.R. and L.K. contributed equally to this study.

Molecular and Cellular Biology, September 2004, p. 8090-8103, Vol. 24, No. 18
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.18.8090-8103.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Goh, S.-L., Looi, Y., Shen, H., Fang, J., Bodner, C., Houle, M., Ng, A. C.-H., Screaton, R. A., Featherstone, M. (2009). Transcriptional Activation by MEIS1A in Response to Protein Kinase A Signaling Requires the Transducers of Regulated CREB Family of CREB Co-activators. J. Biol. Chem. 284: 18904-18912 [Abstract] [Full Text]  
  • Taylor, P., Hardin, P. E. (2008). Rhythmic E-Box Binding by CLK-CYC Controls Daily Cycles in per and tim Transcription and Chromatin Modifications. Mol. Cell. Biol. 28: 4642-4652 [Abstract] [Full Text]  
  • Witcher, M., Pettersson, F., Dupere-Richer, D., Padovani, A., Summers-Deluca, L., Baldwin, A. S., Miller, W. H. Jr (2008). Retinoic acid modulates chromatin to potentiate tumor necrosis factor alpha signaling on the DIF2 promoter. Nucleic Acids Res 36: 435-443 [Abstract] [Full Text]  
  • Hamalainen-Laanaya, H. K., Kobie, J. J., Chang, C., Zeng, W.-p. (2007). Temporal and Spatial Changes of Histone 3 K4 Dimethylation at the IFN-{gamma} Gene during Th1 and Th2 Cell Differentiation. J. Immunol. 179: 6410-6415 [Abstract] [Full Text]  
  • Sessa, L., Breiling, A., Lavorgna, G., Silvestri, L., Casari, G., Orlando, V. (2007). Noncoding RNA synthesis and loss of Polycomb group repression accompanies the colinear activation of the human HOXA cluster. RNA 13: 223-239 [Abstract] [Full Text]  
  • Kahn, T. G., Schwartz, Y. B., Dellino, G. I., Pirrotta, V. (2006). Polycomb Complexes and the Propagation of the Methylation Mark at the Drosophila Ubx Gene. J. Biol. Chem. 281: 29064-29075 [Abstract] [Full Text]  
  • Kobrossy, L., Rastegar, M., Featherstone, M. (2006). Interplay between Chromatin and Trans-acting Factors Regulating the Hoxd4 Promoter during Neural Differentiation. J. Biol. Chem. 281: 25926-25939 [Abstract] [Full Text]  
  • Fujimura, Y.-i., Isono, K.-i., Vidal, M., Endoh, M., Kajita, H., Mizutani-Koseki, Y., Takihara, Y., van Lohuizen, M., Otte, A., Jenuwein, T., Deschamps, J., Koseki, H. (2006). Distinct roles of Polycomb group gene products in transcriptionally repressed and active domains of Hoxb8. Development 133: 2371-2381 [Abstract] [Full Text]  
  • Terranova, R., Agherbi, H., Boned, A., Meresse, S., Djabali, M. (2006). Histone and DNA methylation defects at Hox genes in mice expressing a SET domain-truncated form of Mll. Proc. Natl. Acad. Sci. USA 103: 6629-6634 [Abstract] [Full Text]  
  • Isono, K., Nemoto, K., Li, Y., Takada, Y., Suzuki, R., Katsuki, M., Nakagawara, A., Koseki, H. (2006). Overlapping roles for homeodomain-interacting protein kinases hipk1 and hipk2 in the mediation of cell growth in response to morphogenetic and genotoxic signals.. Mol. Cell. Biol. 26: 2758-2771 [Abstract] [Full Text]  
  • Chen, F.-C., Wang, S.-S., Chen, C.-J., Li, W.-H., Chuang, T.-J. (2006). Alternatively and Constitutively Spliced Exons Are Subject to Different Evolutionary Forces. Mol Biol Evol 23: 675-682 [Abstract] [Full Text]  
  • Deschamps, J., van Nes, J. (2005). Developmental regulation of the Hox genes during axial morphogenesis in the mouse. Development 132: 2931-2942 [Abstract] [Full Text]  
  • Szutorisz, H., Canzonetta, C., Georgiou, A., Chow, C.-M., Tora, L., Dillon, N. (2005). Formation of an Active Tissue-Specific Chromatin Domain Initiated by Epigenetic Marking at the Embryonic Stem Cell Stage. Mol. Cell. Biol. 25: 1804-1820 [Abstract] [Full Text]  
  • Martins, R. P., Ostermeier, G. C., Krawetz, S. A. (2004). Nuclear Matrix Interactions at the Human Protamine Domain: A WORKING MODEL OF POTENTIATION. J. Biol. Chem. 279: 51862-51868 [Abstract] [Full Text]  
  • Haller, K., Rambaldi, I., Daniels, E., Featherstone, M. (2004). Subcellular Localization of Multiple PREP2 Isoforms Is Regulated by Actin, Tubulin, and Nuclear Export. J. Biol. Chem. 279: 49384-49394 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»