This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Oh, J. Articles by Olson, E. N. Search for Related Content PubMed PubMed Citation Articles by Oh, J. Articles by Olson, E. N.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, October 2004, p. 8519-8528, Vol. 24, No. 19
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.19.8519-8528.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Target Gene-Specific Modulation of Myocardin Activity by GATA Transcription Factors

Jiyeon Oh,^1, Zhigao Wang,^1, Da-Zhi Wang,^1,2, Ching-Ling Lien,¹ Weibing Xing,² and Eric N. Olson^1*

Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas,¹ Carolina Cardiovascular Biology Center, Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, North Carolina²

Received 15 July 2003/ Returned for modification 26 August 2003/ Accepted 16 June 2004

Myocardin is a transcriptional coactivator that regulates cardiac and smooth muscle gene expression by associating with serum response factor. We show that GATA transcription factors can either stimulate or suppress the transcriptional activity of myocardin, depending on the target gene. Modulation of myocardin activity by GATA4 is mediated by the physical interaction of myocardin with the DNA binding domain of GATA4 but does not require binding of GATA4 to DNA. Paradoxically, the transcription activation domain of GATA4 is dispensable for the stimulatory effect of GATA4 on myocardin activity but is required for repression of myocardin activity. The ability of GATA transcription factors to modulate myocardin activity provides a potential mechanism for fine tuning the expression of serum response factor target genes in a gene-specific manner.

J.O., Z.W., and D.-Z.W. contributed equally.

This article has been cited by other articles:

• Wang, J., Li, A., Wang, Z., Feng, X., Olson, E. N., Schwartz, R. J. (2007). Myocardin Sumoylation Transactivates Cardiogenic Genes in Pluripotent 10T1/2 Fibroblasts. Mol. Cell. Biol. 27: 622-632 [Abstract] [Full Text]  
• Herring, B. P., El-Mounayri, O., Gallagher, P. J., Yin, F., Zhou, J. (2006). Regulation of myosin light chain kinase and telokin expression in smooth muscle tissues. Am. J. Physiol. Cell Physiol. 291: C817-C827 [Abstract] [Full Text]  
• Xin, M., Davis, C. A., Molkentin, J. D., Lien, C.-L., Duncan, S. A., Richardson, J. A., Olson, E. N. (2006). A threshold of GATA4 and GATA6 expression is required for cardiovascular development. Proc. Natl. Acad. Sci. USA 103: 11189-11194 [Abstract] [Full Text]  
• Pipes, G.C. T., Creemers, E. E., Olson, E. N. (2006). The myocardin family of transcriptional coactivators: versatile regulators of cell growth, migration, and myogenesis.. Genes Dev. 20: 1545-1556 [Abstract] [Full Text]  
• Yin, F., Hoggatt, A. M., Zhou, J., Herring, B. P. (2006). 130-kDa smooth muscle myosin light chain kinase is transcribed from a CArG-dependent, internal promoter within the mouse mylk gene. Am. J. Physiol. Cell Physiol. 290: C1599-C1609 [Abstract] [Full Text]  
• Konhilas, J. P., Leinwand, L. A. (2006). Partnering Up for Cardiac Hypertrophy. Circ. Res. 98: 985-987 [Full Text]  
• Xing, W., Zhang, T.-C., Cao, D., Wang, Z., Antos, C. L., Li, S., Wang, Y., Olson, E. N., Wang, D.-Z. (2006). Myocardin Induces Cardiomyocyte Hypertrophy. Circ. Res. 98: 1089-1097 [Abstract] [Full Text]  
• Davis, C. A., Haberland, M., Arnold, M. A., Sutherland, L. B., McDonald, O. G., Richardson, J. A., Childs, G., Harris, S., Owens, G. K., Olson, E. N. (2006). PRISM/PRDM6, a Transcriptional Repressor That Promotes the Proliferative Gene Program in Smooth Muscle Cells.. Mol. Cell. Biol. 26: 2626-2636 [Abstract] [Full Text]  
• Wei, J., Gorman, T. E., Liu, X., Ith, B., Tseng, A., Chen, Z., Simon, D. I., Layne, M. D., Yet, S.-F. (2005). Increased Neointima Formation in Cysteine-Rich Protein 2-Deficient Mice in Response to Vascular Injury. Circ. Res. 97: 1323-1331 [Abstract] [Full Text]  
• Zhou, J., Hu, G., Herring, B. P. (2005). Smooth Muscle-Specific Genes Are Differentially Sensitive to Inhibition by Elk-1. Mol. Cell. Biol. 25: 9874-9885 [Abstract] [Full Text]  
• Callis, T. E., Cao, D., Wang, D.-Z. (2005). Bone Morphogenetic Protein Signaling Modulates Myocardin Transactivation of Cardiac Genes. Circ. Res. 97: 992-1000 [Abstract] [Full Text]  
• Qiu, P., Ritchie, R. P., Fu, Z., Cao, D., Cumming, J., Miano, J. M., Wang, D.-Z., Li, H. J., Li, L. (2005). Myocardin Enhances Smad3-Mediated Transforming Growth Factor-{beta}1 Signaling in a CArG Box-Independent Manner: Smad-Binding Element Is an Important cis Element for SM22{alpha} Transcription In Vivo. Circ. Res. 97: 983-991 [Abstract] [Full Text]  
• Streb, J. W., Miano, J. M. (2005). AKAP12{alpha}, an Atypical Serum Response Factor-dependent Target Gene. J. Biol. Chem. 280: 4125-4134 [Abstract] [Full Text]  
• Yin, F., Herring, B. P. (2005). GATA-6 Can Act as a Positive or Negative Regulator of Smooth Muscle-specific Gene Expression. J. Biol. Chem. 280: 4745-4752 [Abstract] [Full Text]